Tessera
Folgen

Thomas B. Poulsen

@tbpoulsen.bsky.social

Professor at Aarhus University. Total synthesis of natural products. Covalent chemical biology. Co-founder: Mmimetika Biosciences and Kripthonite Therapeutics

16 Posts1312 Follower534 Folgt
Michael Willis@rhpdcu.bsky.social · last yr.

Great to see Ivan and Charlie's work on the synthesis and reactivity of vinyl sulfonimidamides out in @chemicalscience.rsc.org Here they show that reactivity either above or below that of the corresponding acrylamide can be achieved. @oxfordchemistry.bsky.social pubs.rsc.org/en/content/a...

Synthesis and functionalization of vinyl sulfonimidamides and their potential as electrophilic warheads

Covalent inhibitor design is dominated by the use of electrophilic acrylamide warheads. One limitation of acrylamides is that there are limited opportunities to modify their electrophilicity, and henc...

pubs.rsc.org

Thomas B. Poulsen@tbpoulsen.bsky.social · 2y ago

Here is a very interesting new paper from the Baker and Chudasama groups at University College London. A clever example of leveraging reversible disulfide rebridging chemistry to achieve selective lysine modification (via proximity) of antibody-fragments. pubs.rsc.org/en/content/a...

Formation of mono- and dual-labelled antibody fragment conjugates via reversible site-selective disulfide modification and proximity induced lysine reactivity

Many protein bioconjugation strategies focus on the modification of lysine residues owing to the nucleophilicity of their amine side-chain, the generally high abundance of lysine residues on a protein...

pubs.rsc.org

Eddy Pasquier 🧪🇫🇷🇦🇺@eddpasquier.bsky.social · 2y ago

Now that’s a good #ChemSky thread! Can’t wait to read the final version of this preprint in some top notch journal. Congrats to all involved. Beautiful work 👌🏻

Esben Svenningsen@esbensv.bsky.social · 2y ago

In my first #bsky post, I am very happy and proud to share the new(ish) preprint of my post doc project on N-terminal cysteine (NCys) chemoprotomics using the oxSTEF probes! Preprint at 👉 www.doi.org/10.26434/chemrxiv-2024-4w549 Skeeturial (??) below ⬇️ #ChemSky #ChemBio

Graphical abstract of scientific paper. Chemical structures of oxSTEF probes and N-terminal cysteine, showing their reaction followed by a schematic of a proteomics experiment
Verhelst Lab@verhelstlab.bsky.social · 2y ago

This looks like an interesting application of the oxSTEF probes developed in the Poulsen lab before. Good example of developing interesting chemistry/chemical biology and then asking the right biological questions.

Esben Svenningsen@esbensv.bsky.social · 2y ago

In my first #bsky post, I am very happy and proud to share the new(ish) preprint of my post doc project on N-terminal cysteine (NCys) chemoprotomics using the oxSTEF probes! Preprint at 👉 www.doi.org/10.26434/chemrxiv-2024-4w549 Skeeturial (??) below ⬇️ #ChemSky #ChemBio

Graphical abstract of scientific paper. Chemical structures of oxSTEF probes and N-terminal cysteine, showing their reaction followed by a schematic of a proteomics experiment
Stephan Hacker@stephanhacker2.bsky.social · 2y ago

Interesting ChemRxiv preprint by the group of @tbpoulsen.bsky.social with first author @esbensv.bsky.social. They used OxSTEF probes to study proteins with N-terminal cysteines in the proteome and used the method to specifically investigate difference in normoxia and hypoxia. doi.org/10.26434/che...

Chemoproteomic mapping of the N-terminal cysteinome

The oxidation status of N-terminal cysteines directly dictates protein stability via arginylation and proteasomal degradation. However, only a handful of proteins have been shown to be regulated via t...

doi.org

Stephan Hacker@stephanhacker2.bsky.social · 2y ago

Great to see them apply the proteome-wide profiling method for electrophile reactivity and selectivity that we developed together with @nesvilab.bsky.social in Fragpipe in this exciting system. chemrxiv.org/engage/chemr...

Profiling the proteome-wide selectivity of diverse electrophiles

Targeted covalent inhibitors are powerful entities in drug discovery, but their application has so far mainly been limited to addressing cysteine residues. The development of cysteine-directed covalen...

chemrxiv.org

Thomas B. Poulsen@tbpoulsen.bsky.social · 2y ago

Interested in covalent probes? Check out our most recent pre-print on chemoproteomic profiling of N-terminal cysteines. Nice overview/summary from lead author @esbensv.bsky.social

Esben Svenningsen@esbensv.bsky.social · 2y ago

In my first #bsky post, I am very happy and proud to share the new(ish) preprint of my post doc project on N-terminal cysteine (NCys) chemoprotomics using the oxSTEF probes! Preprint at 👉 www.doi.org/10.26434/chemrxiv-2024-4w549 Skeeturial (??) below ⬇️ #ChemSky #ChemBio

Graphical abstract of scientific paper. Chemical structures of oxSTEF probes and N-terminal cysteine, showing their reaction followed by a schematic of a proteomics experiment
Axel Pahl@apahl.bsky.social · 3y ago

Dear colleagues, my temporary contract here at the MPI is ending this year, and for reasons of german work regulations (Wissenschaftszeitvertragsgesetz, a german word of typical length ;-) ), it can not be extended. So I am looking for new opportunities, preferably in Germany.