If you are interested in resolving the structure of isolated proteins with ~1 nm resolution using Expansion Localization Microscopy with spontaneously blinking dyes (JF635b), then take a look here: ▶️ pubs.acs.org/cbihbp/artic...
Single-Step Expansion SMLM Enables Molecular-Resolution Imaging in Cells and Isolated Proteins
Abstract. Expansion microscopy (ExM) enables nanoscale fluorescence imaging on standard microscopes, but its combination with single-molecule localization
pubs.acs.org