Filip Tomaska
@filip-tomaska.bsky.social
from igluesniffing3 and whalocamping to large FOV diesel2p optomanip. PhD candidate at dyns.ucsb.edu a very nice ride so far '21-now slslab.org at @ucsb.bsky.social '19-'21 Schreiter/Podgorski lab @hhmijanelia.bsky.social
Lighting up your screen: a larval zebrafish's cellular activity. Developed by researchers at our Janelia Research Campus, WHaloCaMP is a biosensor that detects calcium signals — a key part of cellular communication — & offers a window into how cells & organs work together. 🎥 Helen Farrants, HHMI JRC
#kurtosis_summer >>> prime days
Spencer LaVere Smith@spencerlaveresmith.bsky.social · 3mo agoTry out @filip-tomaska.bsky.social 's apps to examine your data and optimize your experiments. Imaging parameter trade-offs: ftomaska.github.io/phase-diagram/ Kurtosis / SNR / gain / photons: github.com/ftomaska/kur... (8/8)
a friendly reminder to enjoy #kurtosis_summer
Spencer LaVere Smith@spencerlaveresmith.bsky.social · 3mo agoA quick check is to examine the kurtosis of traces, at least for L2/3 mouse neurons. This can help you exclude low fidelity cells (i.e. regions of interest / ROIs). @filip-tomaska.bsky.social wrote an app for checking your traces and estimating your photons/s/cell github.com/ftomaska/kur... (5/8)
link.springer.com/article/10.1... #islands_of_positive_kurtosis #self_identified_nepo #nepo_&_proud
The positive side of hunger - EMBO Reports
EMBO Reports - A reflection on how scarcity may shape intellectual discipline and ingenuity in research, and whether today’s easy access to resources may sometimes undermine their thoughtful...
link.springer.com
your L2/3 pyramids have www.youtube.com/watch?v=nBv5... (expectations) #kurtosis_summer
Spencer LaVere Smith@spencerlaveresmith.bsky.social · 3mo agoWe can compute the number of photons/s/neuron, and this tells us how sensitively we can detect small events (changes in fluorescence). @filip-tomaska.bsky.social built a nice app for exploring how imaging parameters affect event detection ftomaska.github.io/phase-diagram/ (3/8)
Hey everyone! Do you remember this post from your fav blogger? labrigger.com/blog/2025/11... Not super easy to use - I know. Well, spring break has sprung here at ucsb and we decided to bring that activation energy down a notch. I guess welcome to my first bsky thread? letsgo (1/5)
Snappy looking calcium imaging videos – Labrigger
labrigger.com
big FOV energy
Spencer LaVere Smith@spencerlaveresmith.bsky.social · 8mo agoTwo-photon calcium imaging at 24,000 lines/s, with the resonant axis spanning 4x what other systems can do. Inertia-free. Diffraction-limited. No tradeoffs. Che-Hang Yu developed a 4x angle multiplier for laser scanning. His paper is out today: opg.optica.org/optica/fullt... 1/n #fluorescenceFriday
iGluSnFR4 is now available @natmethods.nature.com www.nature.com/articles/s41... Glutamate indicators with increased sensitivity and tailored deactivation rates 🧪
Glutamate indicators with increased sensitivity and tailored deactivation rates - Nature Methods
iGluSnFR4f and iGluSnFR4s are the latest generation of genetically encoded glutamate sensors. They are advantageous for detecting rapid dynamics and large population activity, respectively, as demonst...
nature.com
📢 Join us, the Haberkern lab, @uni-wuerzburg.de for a postdoc studying neural circuit mechanisms of navigation. You’ll spearheading neurophysiology experiments on our brand new 2P! ⏳ Apply by 28th February 2026 Details: www.haberkernlab.de/docs/ENPostd... #neuroscience #academicjobs #postdoc
🚨iGluSnFR4 is finally out!🚨🧪 We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️ www.biorxiv.org/content/10.1... 🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX #Neuroscience
Maximal support but only self generated starter packs count go.bsky.app/3vKYJLF
Lucina Uddin@lucinauddin.bsky.social · 2y agoProposal to use 'how many starter packs a researcher is included in' as the new h-index.
Just a piece of cortex I saw under the microscope today 🤓🔬 Cell types: neurons 🔵, astrocytes 🟡, microglia 🟣. Observe how astrocytes are covering two long blood vessels, crossing all the gray matter and almost reaching white matter. The brain is so pretty!
This place sort of feels like the science Twitter I used to like. Maybe even better? Fingers crossed. Wish I had a fresh preprint to share, but here's the next best thing: a fresh osprey with some blue sky background.
