Delighted that our story on chromatin architecture inside human cells is now published — the last chapter of my PhD. It was a pleasure working with @sergiocruzleon.bsky.social, @johannesbetz.bsky.social, and everyone involved. #teamtomo www.nature.com/articles/s41...

Molecular architecture of heterochromatin at the nuclear periphery of primary human cells - Nature Communications

The 3D organisation of chromatin inside a human cell is still not fully resolved. Here the authors reveal that in situ cryo-ET resolves individual nucleosomes and predicts DNA linkers, thus showing th...

nature.com

Max Planck Institute of Biophysics@mpibp.bsky.social · 3w ago

How do 2 meters of DNA fit inside a nucleus you can barely see? 🧬 Our new study mapped individual nucleosomes inside human cells.

What is the best way to distribute the electron dose across your tilt series in cryo ET? Thanks to Maarten Tuijtel, we finally know --> head over to eLife for the publication! doi.org/10.7554/eLif...

Max Planck Institute of Biophysics@mpibp.bsky.social · last mo.

1/3: We are excited to share a new study by Maarten Tuijtel from @becklab.bsky.social, published in eLife. 🔬 🎉 The work addresses a key question in cryo-electron tomography: how to best distribute the limited electron dose across a tilt series while balancing angular sampling and image quality. 💥

📢 (1/2) Excited to share the first preprint from my PostDoc! We developed UbSeRP to map co-translational ubiquitination in a translatome-wide, revealing thousands of quality control sites, and how they reshape with aging. www.biorxiv.org/content/10.6... #proteostasis #ribosomes #preprint

Ubiquitin selective ribosome profiling reveals systematic principles of co-translational quality control

Protein biogenesis is a stress- and error-sensitive process that can lead to nascent protein misfolding and aggregation, challenging cellular proteostasis. Co-translational ubiquitination (CTU) is a c...

biorxiv.org

Komm’ am 12. Juni auf die Night of Science✨! Erlebe, wie aus Stickstoff leckeres Eis🍦wird und welche Aufgaben verschiedene Proteine in unserem Körper übernehmen! Erfahre in Vorträgen und bei einer Elektronenmikroskopie-Tour 🔬 mehr über unsere Forschung! Infos: 👉 www.biophys.mpg.de/de/night-of-...

Sub-cellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs doi.org/10.1038/s415...

Out now in Nature Communications! We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky www.nature.com/articles/s41...

I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...

Spang Lab@spanglab.bsky.social · 4mo ago

What if you could cut out sub cellular structures and measure the proteome? SPEx: Compartment-Resolved Proteomics via Expansion Microscopy–Guided Microdissection www.biorxiv.org/content/10.6... awesome work by Curdin Franziscus, @imcf-lex.bsky.social, @biehli.bsky.social, Alexander Schmidt!

Updated architectural model of the NPC from our lab, starred by bsky-less Agnieszka Obarska-Kosińska. Using cryo ET, XL-MS and modeling, we identify novel nucleoporins, among those the mRNA export complex TREX-2! Check out the preprint on biorxiv #TeamMassSpec #TeamTomo

bioRxivpreprint@biorxivpreprint.bsky.social · 5mo ago

How the TREX-2 complex associates with the nuclear pore https://www.biorxiv.org/content/10.64898/2026.02.20.707084v1

Mammalian cells form hibernating disomes akin to those in bacterial cells - but connected via ribosomal RNA www.science.org/doi/10.1126/... co-first with: @andschwarz.bsky.social with: @lea-dietrich.bsky.social, @sgiando.bsky.social, @erin-schuman.bsky.social and many more 🧵 0/6 #MolBio 🧪

Ready to TANGO? 💃 In our new publication, Beata Turoňová & Markus Schreiber (@ms314.bsky.social, @becklab.bsky.social) present TANGO: the first cryoET framework to analyze the relation between particle positions and orientations 🪩 All in a single user-friendly package! #CryoET #OpenScience

Now online! Metabolically regulated proteasome supramolecular organization in situ

Metabolically regulated proteasome supramolecular organization in situ

Structural steps along the assembly of proteasome storage granules—membraneless organelles that form in response to metabolic shifts in yeast—are visualized inside cells by cryo-electron tomography. Inactive 26S proteasomes oligomerize into trimers, which assemble into paracrystalline arrays that serve as reservoirs of fully assembled proteasomes under conditions of low energy, ready for reactivation when glucose is restored.

dlvr.it

LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery. Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure. 🔗 doi.org/10.64898/202... #CryoET

This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell! This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍 [Maybe long thread ahead]

Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii

Using the latest advances in instrumentation and computational workflows, Kelley et al. present a large-scale annotated cryo-electron tomography dataset of the model green alga, Chlamydomonas reinhard...

cell.com

Molecular Cell @cp-molcell.bsky.social · 7mo ago

The latest issue is now online www.cell.com/molecular-ce...

Take advantage of this fantastic opportunity to become part of the SCALE community! Several professorships and group leader positions are available at Goethe University and FIAS. We are looking for professors in the fields of molecular microbiology, cellular biochemistry, and molecular biochemistry.

SCALE - SubCellular Architecture of LifE@scale-cluster-ffm.bsky.social · 8mo ago

⏰Call for Applications - Join Our Team and Help Shape Our Cluster @goetheuni.bsky.social and the Cluster of Excellence SCALE - Subcellular Architecture of Life are seeking 3 highly motivated scientists to join a vibrant and innovative research community on Campus Riedberg. Share with your network!

It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

bioRxivpreprint@biorxivpreprint.bsky.social · 8mo ago

The vault associates with membranes in situ https://www.biorxiv.org/content/10.64898/2025.12.12.693869v1

Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...

❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...

cryoTIGER: deep-learning based tilt interpolation generator for enhanced reconstruction in cryo electron tomography - Communications Biology

Deep learning-based interpolation using cryoTIGER enhances angular sampling in cryoET, improving 3D reconstructions, particle localization, and structural recovery without increasing electron dose.

nature.com

great lipid probes promising exciting studies for (membrane) cell bio, such fascinating work!

Nature@nature.com · 12mo ago

Nature research paper: Quantitative imaging of lipid transport in mammalian cells go.nature.com/4mmSygd

The 3-decade-old competition that enabled the emergence and evaluate of AlphaFold has run out of NIH funding. The program will be terminated in weeks. This is terminating success. www.science.org/content/arti...

🎉 We’re absolutely thrilled: SCALE has been selected as a DFG Excellence Cluster! A huge thank you to our incredible team—this would not have been possible without your dedication and talent. 🙌 @dfg.de #ClustersOfExcellence #SCALEcluster 🧵

Beautiful work by JP, Sergio, Johannes and colleagues!

Jan Philipp Kreysing@jpkreysing.bsky.social · last yr.

New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇

Excited to share our latest work on nuclear pore complex scaffold integrity and its perturbation. Now out in Nature Cell Biology: rdcu.be/eg4i9 Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳

Nuclear pores safeguard the integrity of the nuclear envelope

Nature Cell Biology - Taniguchi et al. structurally analyse nuclear pore complex architecture in situ during differentiation, which is associated with mechanical constraints on the nuclear...

rdcu.be

Another method from our lab on biorxiv! Automated classification and removal of flawed tilts in cryo ET data

bioRxivpreprint@biorxivpreprint.bsky.social · last yr.

Automated Removal of Corrupted Tilts in Cryo-Electron Tomography https://www.biorxiv.org/content/10.1101/2025.03.13.642992v1

Check out @deglushk.bsky.social 's method to measure membrane thickness in cryo electron tomograms!

bioRxivpreprint@biorxivpreprint.bsky.social · last yr.

In situ evidence for systematic membrane thickness variation across cellular organelles https://www.biorxiv.org/content/10.1101/2025.03.13.642912v1