Starting off my 29th Fresher’s week with a proud supervisor moment. Congratulations to Imogen for successfully defending her PhD thesis. Thanks to Wyatt Yue and Paul James for examining and a special thanks to Dennis the support dog!

Starting off the new academic year with publication a long time in the making: doi.org/10.1016/j.js... The high-resolution analysis of the cargo couldn't have been achieved without us publishing previous raw data and a great tutorial from the team @cryosparc.bsky.social.

Our study on Bacteroides iron piracy is now out in @pnas.org. We show how surface-exposed lipoproteins bind xenosiderophores with different iron-chelating chemistries and propose a mechanism for xenosiderophore import across the outer membrane. Lots of structures (mostly X-ray this time)!

Structural basis of iron piracy by human gut Bacteroides | PNAS

Iron is an essential element that can be growth-limiting in microbial communities, particularly those present within host organisms. To acquire iro...

doi.org

We have a PhD studentship open! (UK only sadly) but if you are or know a student keen to work on some *really* weird mycobacterial enzymes then get in touch! #glycotime Advert below www.findaphd.com/phds/project...

Uncovering a new class of toxins in pathogenic mycobacteria at Newcastle University on FindAPhD.com

PhD Project - Uncovering a new class of toxins in pathogenic mycobacteria at Newcastle University, listed on FindAPhD.com

findaphd.com

What are the genetic and molecular determinants underpinning the repair of bidirectional replication fork collapse in bacteria? PhD position open in my lab in collaboration with the brilliant @heathmurraylab.bsky.social. Please share and contact me if interested: www.findaphd.com/phds/project...

Identifying bacterial DNA repair requirements enabling stable genetic inheritance during bidirectional replication fork collapse at Newcastle University on FindAPhD.com

PhD Project - Identifying bacterial DNA repair requirements enabling stable genetic inheritance during bidirectional replication fork collapse at Newcastle University, listed on FindAPhD.com

findaphd.com

Happy April 25th all who celebrate. Light jacket season is upon us.

a woman is holding a microphone and wearing a sash that says ' rhol ' on it

ALT: a woman is holding a microphone and wearing a sash that says ' rhol ' on it

media.tenor.com

We’ve been sciencing! M abscessus makes a arabinanase toxin to chop up the cell wall of competitor mycobacteria! #glycotime 🦠 thanks to @wellcometrust.bsky.social for the funding that makes this collaborative work possible! Also Sam’s structures are 😍 and Jean-Lou did some lovely biochem

Patrick Moynihan@pmoyniha.bsky.social · 7mo ago

We've been we've been working on this for quite a while now (hopefully published soon). Grateful to @proftracypalmer.bsky.social for concinving me & @lislowe.bsky.social that mycobacteria produce inter-bacterial toxins. Team science, led by @sambenedict5.bsky.social www.biorxiv.org/content/10.6...

www.findaphd.com/phds/project... interested in bacterial antagonism? PhD opportunity in our team, see below. Please repost!

Characterising antibacterial toxins in the food-borne pathogen Listeria monocytogenes at Newcastle University on FindAPhD.com

PhD Project - Characterising antibacterial toxins in the food-borne pathogen Listeria monocytogenes at Newcastle University, listed on FindAPhD.com

findaphd.com

BBSSRC (NEEDL) funded PhD studentship in my lab co-supervised by @thecopperdoctor.bsky.social and @mycobacterium-ncl.bsky.social If you're interested in structural biology and membrane transport processes, check out www.findaphd.com/phds/project... deadline 27th of Feb! Get in touch for more info

Exciting CASE funded PhD opportunity available in the NEEDL PhD programme with me and Thomas Howard: eur03.safelinks.protection.outlook.com?url=https%3A...

Synthetic Biology for Light-Activated Smart Materials. at Newcastle University on FindAPhD.com

PhD Project - Synthetic Biology for Light-Activated Smart Materials. at Newcastle University, listed on FindAPhD.com

eur03.safelinks.protection.outlook.com

We are looking for a postdoc to work on mechanisms of #RNA decay in cancer using #cryoEM with #nanobodies and #minibinders! Please RT

🚨Last chance to apply for two PhD projects in my lab, applying structural biology and single molecule biophysics to investigate gene expression in RNA viruses: www.findaphd.com/phds/?Keywor...

www.nature.com/articles/s41...

AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structure determination - Nature Methods

An analysis of AlphaFold protein structure predictions shows that while in many cases the predictions are highly accurate, there are also many instances where the predicted structures or parts of pred...

nature.com

Please RT. Post-doc opportunity alert! 💥 closing soon 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems. For more details and to apply please see www.imperial.ac.uk/jobs/search-...

Are you looking for female speakers in CryoEM for seminars and conferences? Are you a woman in CryoEM and not yet on the ‚woman in CryoEM‘ list? Find your speakers and add/update name and affiliation! docs.google.com/spreadsheets...

Proud and excited to see my first, first-author paper out on @pnas.org! Huge thank you to Jon @jmarlesw.bsky.social, Luning and my colleagues for their amazing guidance throughout. www.pnas.org/doi/10.1073/...

Structure and encapsulation of carbonic anhydrase within the α-carboxysome | PNAS

Carboxysomes in cyanobacteria and certain proteobacteria enable efficient CO2 fixation by encapsulating ribulose-1,5-bisphosphate carboxylase/oxyge...

pnas.org

Jon Marles-Wright@jmarlesw.bsky.social · 11mo ago

Pleased to see this collaboration with a great PhD student Pei Cing Ng, from Prof. Luning Liu's lab at Liverpool University published. Structure of the H. neapolitanus carbonic anhydrase and localisation in a mini-carboxysome: www.pnas.org/doi/epdf/10....

Assembly, architecture and functional roles of microbial surface layers Review article published in @natrevmicro.nature.com with @bupbuse.bsky.social, Andriko von Kügelgen and @vikramalva.bsky.social. S-layers are everywhere! www.nature.com/articles/s41...

Assembly, architecture and functional roles of microbial surface layers - Nature Reviews Microbiology

Surface layers (S-layers) are ubiquitous protein assemblies that coat prokaryotic cells, with their functional roles increasingly coming into focus. In this Review, Isbilir and colleagues discuss rece...

nature.com

Pleased to see this collaboration with a great PhD student Pei Cing Ng, from Prof. Luning Liu's lab at Liverpool University published. Structure of the H. neapolitanus carbonic anhydrase and localisation in a mini-carboxysome: www.pnas.org/doi/epdf/10....

I am excited to share our new preprint on our structural and biochemical analyses of human methionine synthase (MTR). This was the hard work of Douglas Ferreira who carried out this research as a significant part of his PhD. #CryoEM www.biorxiv.org/content/10.1...

Structural insights into cobalamin loading and reactivation of human methionine synthase

Human methionine synthase (MTR) is an essential enzyme of one carbon metabolism. Consisting of a catalytic N-half and a cobalamin binding C-half, MTR utilises this intricate organometallic cofactor in...

biorxiv.org

Afternoon starts with former #Salgadolab student @adamcrawshaw.bsky.social getting the audience engaged on #microcrystals and @diamondlightsource.bsky.social VMXm beamline.

Another exciting day in Newcastle for structural biology! The CCP4 Structural Biology Meeting 2025 is here!

A great overview of #cryoEM in Newcastle from @jmarlesw.bsky.social at #NorthernEye opening @nusbf.bsky.social

Woo. The big day is finally here. @jmarlesw.bsky.social kicking off the Northern Eye Opening Symposium

Please join us in the North East on the 5th of November as we celebrate with a one day symposium for the launch of our BBSRC funded 100 keV Tundra TEM aka the Northern Eye: nusbf.ncl.ac.uk/ccpem-tfs-ne... The symposium has been kindly sponsored by @thermofishersci.bsky.social & @ccpem.bsky.social

Interested in alpha-oxoamine synthases and their use in biotech? Here's a nice methods paper on the production and characterisation of an engineered T. maritima variant from colleagues in the @campopianogroup.bsky.social: www.sciencedirect.com/science/arti...

Characterisation and engineering of thermostable PLP-dependent alpha-oxoamine synthases (AOSs); versatile C-C bond forming biocatalysts

The toolbox of C-C bond forming biocatalysts is forever expanding as demand increases for new and sustainable routes to organic molecules. One class o…

sciencedirect.com

The Quinn group are interested in stress-sensing and signalling mechanisms in human pathogenic fungi and their importance in virulence. They are also interested anti-fungal mechanisms mediated by the bacterial type-vi secretion system. 👉 @nclfungalgroup.bsky.social www.ncl.ac.uk/medical-scie...

The Palmer group are interested in the mechanisms bacteria use to compete with one another during colonisation. Her group are focused on the type vii secretion system and the toxins it exports. Follow @proftracypalmer.bsky.social for more insights. www.ncl.ac.uk/medical-scie...

Next up, @nickj-dent-res.bsky.social His group is interested in all aspects of oral biofilms and how they contribute to oral health and disease. They look at adhesion and colonisation of surfaces, intermicrobial interactions and the role of the biofilm matrix. blogs.ncl.ac.uk/nickjakubovi...

Next introducing Dr Alessandra Dantas. The Dantas group is interested in stress responses, antifungal resistance and in understanding the molecular mechanisms driving fungal adaptation to environmental stressors. @alesdantas.bsky.social www.ncl.ac.uk/dental/about...

Last introduction for the today.. Dr Kesha Josts @kjosts.bsky.social The Josts lab are working on the mechanisms of iron transport in bacterial pathogens, with a focus on Pseudomonas aeruginosa. www.ncl.ac.uk/cbcb/staff/p...