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Tosin Q. Orababa

@tosinorababa.bsky.social

Postdoctoral Research Fellow || AMR and Biofilm infections || Ancientbiotics

13 Posts386 Follower912 Folgt
Freya Harrison@friendlymicrobe.bsky.social · last mo.

Low concentrations of tetrasodium EDTA kill P. aeruginosa biofilm in some, but not all, chronic infection models. New paper proposed & designed by @tosinorababa.bsky.social, who led a team of PhD students to get this research done www.microbiologyresearch.org/content/jour... #MicroSky

Low concentrations of tetrasodium EDTA cause significant killing of biofilm-associated Pseudomonas aeruginosa in high-validity models of chronic wound and cystic fibrosis lung infections – but not in ...

Pseudomonas aeruginosa is a pathogen notorious for its antimicrobial resistance and is currently classified as a high-priority pathogen for which new drugs are needed. Tetrasodium EDTA (tEDTA) is one ...

microbiologyresearch.org

Freya Harrison@friendlymicrobe.bsky.social · 2mo ago

Does media matter? RNAseq of S. aureus growing in LB broth vs. synthetic wound fluid shows differential expression of genes involved in efflux, virulence & ion transport. Work led by @tosinorababa.bsky.social: his first manuscript from independent funding! #MicroSky www.biorxiv.org/content/10.6...

Does media matter? Growth environment influence antimicrobial tolerance and expression of virulence and transmembrane ion transport-associated genes in MRSA

Clinically relevant pathogens are often tested for antimicrobial susceptibility using standard laboratory media that poorly reflect the in vivo environments in which they cause infections, leading to ...

biorxiv.org

Tosin Q. Orababa@tosinorababa.bsky.social · 6mo ago

This mechanism of action includes bacterial membrane disruption, nucleic acid biosynthesis inhibition via the inhibition of de novo purine and pyrimidine pathway, quorum sensing inhibition, and inhibition of bacterial adhesion (2/n).

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Tosin Q. Orababa@tosinorababa.bsky.social · 6mo ago

Excited to share our preprint that describes the mechanism of action of an historical infection remedy called Bald’s Eyesalve. In this preprint, we show that Bald’s Eyesalve has multifaceted mechanism of action against both S. aureus and A. baumannii (1/n).

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Freya Harrison@friendlymicrobe.bsky.social · 6mo ago

Remember the medieval remedy we reconstructed years ago? We've now dissected the various ways it attacks and kills bacteria! Preprinted & submitted, led by @tosinorababa.bsky.social & Jess Furner-Pardoe w/ many collaborators #MicroSky #Ancientbiotics www.biorxiv.org/content/10.6...

A cartoon showing the various targets of reconstructed eyesalve against Gram-positive and Gram-negative bacteria. It targets the cell membrane, as well as several intracellular targets including nucleotide biosynthesis. In S. aureus, it also affect quorum sensing.
Tosin Q. Orababa@tosinorababa.bsky.social · 6mo ago

Excited to share our preprint that describes the mechanism of action of an historical infection remedy called Bald’s Eyesalve. In this preprint, we show that Bald’s Eyesalve has multifaceted mechanism of action against both S. aureus and A. baumannii (1/n).

Bild
Freya Harrison@friendlymicrobe.bsky.social · 6mo ago

Remember the medieval remedy we reconstructed years ago? We've now dissected the various ways it attacks and kills bacteria! Preprinted & submitted, led by @tosinorababa.bsky.social & Jess Furner-Pardoe w/ many collaborators #MicroSky #Ancientbiotics www.biorxiv.org/content/10.6...

A cartoon showing the various targets of reconstructed eyesalve against Gram-positive and Gram-negative bacteria. It targets the cell membrane, as well as several intracellular targets including nucleotide biosynthesis. In S. aureus, it also affect quorum sensing.
Freya Harrison@friendlymicrobe.bsky.social · 6mo ago

Why should microbiologists be interested in historical infection remedies, and how can we best investigate them for antimicrobial discovery? Thoroughly enjoyed co-writing this Microbiology Primer with @tosinorababa.bsky.social www.microbiologyresearch.org/content/jour... #MicroSky #Ancientbiotics

Microbial Primer: Ancientbiotics – making modern antimicrobials from historical infection remedies

The modern antibiotic era began in the early twentieth century, but humans have long used materials from the natural world to attempt to treat the symptoms of infection. In this primer, we will discus...

microbiologyresearch.org

Tosin Q. Orababa@tosinorababa.bsky.social · 7mo ago

pubmed.ncbi.nlm.nih.gov/37117030/ Does anyone here have access to the full text of this paper🙏? I have tried emailing the authors but didn’t get any response (more than a month now). Thank you in advance

Construction of a Staphylococcus aureus Gene-Deletion Allelic-Exchange Plasmid by Gibson Assembly and Recovery in Escherichia coli - PubMed

We present a protocol for the generation of a gene-deletion allelic-exchange plasmid and its recovery in <i>Escherichia coli</i> for the purpose of constructing an in-frame gene deletion in <i>Staphylococcus aureus</i> Here, we present detailed methodologies for (i) the primer design (using the <i>S</i> …

pubmed.ncbi.nlm.nih.gov

Tosin Q. Orababa@tosinorababa.bsky.social · 7mo ago

pubmed.ncbi.nlm.nih.gov/37117030/ Does anyone here have access to the full text of this paper🙏? I have tried emailing the authors but didn’t get any response (more than a month now). Thank you in advance

Construction of a Staphylococcus aureus Gene-Deletion Allelic-Exchange Plasmid by Gibson Assembly and Recovery in Escherichia coli - PubMed

We present a protocol for the generation of a gene-deletion allelic-exchange plasmid and its recovery in <i>Escherichia coli</i> for the purpose of constructing an in-frame gene deletion in <i>Staphylococcus aureus</i> Here, we present detailed methodologies for (i) the primer design (using the <i>S</i> …

pubmed.ncbi.nlm.nih.gov

Tosin Q. Orababa@tosinorababa.bsky.social · 8mo ago

Excited to have been selected as an Infection Science Awardee for 2025 by the Microbiology Society. Looking forward to presenting my research at this year’s Federation of Infection Science Conference in Bournemouth.

Microbiology Society@microbiologysociety.org · 8mo ago

🎉 Congratulations to Oluwatosin Orababa @tosinorababa.bsky.social, one of the winners of our 2025 Infection Science Award! 🦠👏 Get to know them in our new interview: microb.io/3XFyCui #InfectionScience #Microbiology #ResearchSpotlight

Photo of Oluwatosin Orababa - Finalist of the 2025 Infection Science Award
Tosin Q. Orababa@tosinorababa.bsky.social · 8mo ago

Excited to have been selected as an Infection Science Awardee for 2025 by the Microbiology Society. Looking forward to presenting my research at this year’s Federation of Infection Science Conference in Bournemouth.

Microbiology Society@microbiologysociety.org · 8mo ago

🎉 Congratulations to Oluwatosin Orababa @tosinorababa.bsky.social, one of the winners of our 2025 Infection Science Award! 🦠👏 Get to know them in our new interview: microb.io/3XFyCui #InfectionScience #Microbiology #ResearchSpotlight

Photo of Oluwatosin Orababa - Finalist of the 2025 Infection Science Award
Freya Harrison@friendlymicrobe.bsky.social · 9mo ago

Low doses of tEDTA kill P. aeruginosa in models of chronic wound and CF lung #biofilm, but not in a model of endotracheal tube colonisation: preprint from a project led by @tosinorababa.bsky.social and implemented by a crack team of PhD students! #MicroSky www.biorxiv.org/content/10.1...

Low concentrations of tetrasodium EDTA cause significant killing of biofilm-associated P. aeruginosa in high validity models of chronic wound and CF lung infections but not in a model of endotracheal tube colonisation

Pseudomonas aeruginosa is a pathogen notorious for its antimicrobial resistance and is currently classified as a high-priority pathogen for which new drugs are needed. Tetrasodium EDTA (tEDTA) is one of the new antimicrobial compounds that have been shown to have good antibacterial and antibiofilm efficacy against P. aeruginosa. Due to the diversity and highly drug-tolerant nature of P. aeruginosa biofilms in different infection environments, it is important to carry out pre-clinical testing of new antibiofilm agents against this pathogen in media and models that accurately mimic diverse infection environments. In this study, we used different high validity media and biofilm models that mimic chronic wounds, endotracheal tubes, and cystic fibrosis lung infections to assess the efficacy of tEDTA against P. aeruginosa biofilms. We report that different infection environments influence the susceptibility of both planktonic and biofilm forms of P. aeruginosa to tEDTA. The highest tolerance to tEDTA was observed in the media and biofilm model that mimics the endotracheal tube environment. In conclusion, we show that although different infection environments influence the efficacy of tEDTA against P. aeruginosa biofilms, it has good potential for use as an alternative antimicrobial in treating P. aeruginosa-associated biofilm infections. ### Competing Interest Statement The authors have declared no competing interest.

biorxiv.org

@aaroncurtismicro.bsky.social · last yr.

Delighted to share our latest publication from @medmycology.bsky.social adapting the EVPL model to study A. fumigatus virulence, made possible through collaboration with @friendlymicrobe.bsky.social. Many thanks to @researchireland.ie for funding this work www.tandfonline.com/doi/full/10....

Proteomic characterization of Aspergillus fumigatus – host interactions using the ex-vivo pig lung (EVPL) model

Aspergillus fumigatus is an opportunistic fungal pathogen of the human airway that can cause a variety of chronic infections, typically in the context of pre-existing lung damage. The interaction o...

tandfonline.com