AMPK phosphorylation proceeds through hierarchical proteoform cascades revealed by integrated mass spectrometry | Science Advances www.science.org/doi/10.1126/.... Congrats to my Marie Curie postdoc fellow, Boris, and all co-authors! 👏 @science.org #kinase #AMPK @proteoforms.bsky.social

AMPK phosphorylation proceeds through hierarchical proteoform cascades revealed by integrated mass spectrometry

Protein kinases integrate cellular signals through complex phosphorylation cascades, yet resolving how chemical perturbations trigger and modulate these cascades in therapeutic targets remains a major...

science.org

Announcing the new leadership team for the Early Career Researcher Committee, providing education and outreach for top-down proteomics. We also thank the previous team for their outstanding efforts in growing the community. Details here > ctdp.org/about-the-co... #proteomics #ASMS2026 #proteoforms

I will be presenting at Consortium for Top-Down Proteomics’s Proteoform Thursday Webinar Series. The catenin phospho-code work laid the foundation for my future research group. Free register link below!

Consortium for Top-Down Proteomics@proteoforms.bsky.social · 7mo ago

Join us March 26 for Proteoform Thursday: Jeffrey Huang presents - "A Catenin Phospho-Code for Adherens Junction Organization" us06web.zoom.us/meeting/regi... #proteomics #massspec

I am thrilled to share that I will be joining @tamuchemistry.bsky.social as a tenure-track Assistant Professor this summer. My research group will advance top-down mass spectrometry and proteomics to study the proteoform biology of cell–cell adhesion and phosphorylation in disease.

Join us January 22 for Proteoform Thursday: Pei Su of UC Riverside presents "From Tissues to Single Cells: Direct Proteoform Profiling Using Orbitrap-Based Single Molecule Mass Spectrometry" #proteomics #proteoform #massspec us06web.zoom.us/meeting/regi...

Wonderful native individual ion mass spectrometry work!

BioMassSpec@realbiomassspec.bsky.social · 10mo ago

Dissecting Heterogeneous Populations of Protein-Complex Samples Using Direct Mass Technology #AC pubs.acs.org/doi/10.1021/...

It was a great pleasure attending my first #HUPO world congress in Toronto last week and sharing our catenin phospho-code story! It was inspiring meeting with peers and pioneers in the field, which reminded me how special the proteomics community is!

THRILLED to share our new publication in @angewandtechemie.bsky.social from #CLP & @nucdb.bsky.social "Intact Mass Profiling Reveals Phospho-Proteoforms of the Catenins (85–110 kDa) Regulated by Actomyosin Contractility” using TDMS to study LARGE catenin proteoforms. (1/7) doi.org/10.1002/anie...

Intact Mass Profiling Reveals Phospho‐Proteoforms of the Catenins (85–110 kDa) Regulated by Actomyosin Contractility

This study advances top-down individual ion mass spectrometry (I2MS) to profile intact phospho-proteoforms of β- and α-catenins (85–110 kDa) within cadherin–catenin complexes. By modulating actomyosi....

doi.org

Heading to #HUPO2025! I’ll talk about our catenin phospho-code story and how top-down MS reveals how adhesion proteins respond to actomyosin force! Thanks for the invite, #AFFINISEP! Full story on bioRxiv 👉https://doi.org/10.1101/2025.09.06.674621

Neil Kelleher: The Human Proteoform Project Could Transform Medicine theanalyticalscienti... --- #proteomics #prot-article

🚨 New in @naturemethods.bsky.social #CHIMERYS has already transformed #proteomics since 2022, powering DDA, DIA & PRM analysis in a single workflow. Wanna know how CHIMERYS works? 👉 www.nature.com/articles/s41... #TeamMassSpec #NatureMethods #ASMS2025 🧪🔬 1/7

High-performance proteomics at any chromatographic flow rate These data follow the expected trends as though taken from a textbook ! They illustrate clearly the trade-offs between high and low flow rates.

Sigh…

Jeremy Berg@jeremymberg.bsky.social · last yr.

STAT story about freezing all payments to Columbia, Harvard, Brown, Northwestern, Cornell, and Cornell-Weill Medicine. www.statnews.com/2025/04/18/n...

🧪Phuong Le, Jeanne Quinn, @jfhorner.bsky.social @carajgottardi.bsky.social & co show that the adhesion protein α-catenin has a key modification that allows dividing cells to stay better connected to their neighbours, helping the tissue stick together during mechanical stress. doi.org/10.1242/bio....

The mass spectrometry research community at Chicago is thriving! Many thanks to the Chicago Mass Spec Interest Group for the invitation to share my catenin proteoform story in the inaugural event. It was a pleasure to meet with researchers at the beautiful CZbiohub space!

Looking forward to sharing my work at the Chicago Mass Spec Interest Group’s inaugural meeting!

It was a very fruitful #USHUPO2025 ! I was beyond thrilled to share our advances in catenin proteoform profiling and functional studies that will reveal the phospho-code in cell-cell adhesions. Many thanks to the conference organizers and participants!

Next steps for targeted protein degradation: Cell Chemical Biology www.cell.com/cell-chemica... #TPD #PROTACs

Next steps for targeted protein degradation

Modalities and applications of targeted protein degradation (TPD) have rapidly expanded the therapeutic possibilities of proximity induced pharmacology. Here, Krone et al. spotlight three focal points...

cell.com

Looking forward to attending the #USHUPO conference in Philly this weekend and sharing some early results in catenin proteoform profiling and our vision to build a story behind acto-myosin contractility regulation in cell-cell adhesion. Please join my talk next Wednesday and say hi!

New manuscript from the lab to establish a Top Down MS platform using electron activated dissociation on the Sciex ZenoTOF 7600 is out at the Journal of Proteome Research! pubs.acs.org/doi/full/10....

Establishing a Top-Down Proteomics Platform on a Time-of-Flight Instrument with Electron-Activated Dissociation

Top-down proteomics is the study of intact proteins and their post-translational modifications with mass spectrometry. Historically, this field is more challenging than its bottom-up counterpart because the species are much bigger and have a larger number of possible combinations of sequences and modifications; thus, there is a great need for technological development. With improvements in instrumentation and a multiplicity of fragmentation modes available, top-down proteomics is quickly gaining in popularity with renewed attention on increasing confidence in identification and quantification. Here, we systematically evaluated the Sciex ZenoTOF 7600 system for top-down proteomics, applying standards in the field to validate the platform and further experimenting with its capabilities in electron-activated dissociation and post-translational modification site localization. The instrument demonstrated robustness in standard proteins for platform QC, as aided by zeno trapping. We were also able to apply this to histone post-translational modifications, achieving high sequence coverage that allowed PTM’s site localization across protein sequences with optimized EAD fragmentation. We demonstrated the ability to analyze proteins spanning the mass range and included analysis of glycosylated proteins. This is a reference point for future top-down proteomics experiments to be conducted on the ZenoTOF 7600 system.

pubs.acs.org

Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers #NatProtoc www.nature.com/articles/s41...

Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers - Nature Protocols

Orbitrap-based individual ion mass spectrometry enables charge detection mass spectrometry application on a broadly accessible mass spectrometry platform, enabling the analysis of complex mixtures tha...

nature.com

It was a tremendous pleasure to visit my alma mater National Taiwan University and give a talk on top-down proteomics and proteoform biology at the Institute of Biomedical Sciences earlier this week.

Advancements in Global Phosphoproteomics Profiling: Overcoming Challenges in Sensitivity and Quantification analyticalsciencejou... --- #proteomics #prot-paper

Emerging opportunities for intact and native protein analysis using chemical proteomics www.sciencedirect.co... --- #proteomics #prot-paper

I love it when a place plays the Nutcracker during the holiday season, not All I Want For Christmas Is You on repeat 😝

Multi-Reflecting TOF MS for Analyzing Proteins #IJMS www.sciencedirect.com/science/arti...

Multi-Reflecting TOF MS for Analyzing Proteins

This paper presents detailed results of previously reported protein studies conducted using a prototype multi-reflecting time-of-flight mass spectrome…

sciencedirect.com

Intact Mass Proteomics Using a Proteoform Atlas #JProteomeRes pubs.acs.org/doi/10.1021/...

Intact Mass Proteomics Using a Proteoform Atlas

Top-down proteomics, the characterization of intact proteoforms by tandem mass spectrometry, is the principal method for proteoform characterization in complex samples. Top-down proteomics relies on p...

pubs.acs.org

Very happy to share my third major work this year (and first on 🦋)! This is a co-first work with the Fitzgerald group at Duke where they perform the SPROX protein folding stability assay and I figure out how to decribe the results using top-down proteomics! doi.org/10.1021/acs....

Top-Down Stability of Proteins from Rates of Oxidation (TD-SPROX) Approach for Measuring Proteoform-Specific Folding Stability

The crucial roles of proteoforms in biological processes and disease mechanisms have been increasingly recognized. However, the rate at which new proteoforms are being discovered using top-down proteomics has far outpaced the rate at which the functional significance of different proteoforms can be determined. Because of the close connection between protein folding and protein function, protein folding stability measurements on proteoforms have the potential to identify functionally significant proteoforms of a given protein. While a number of mass spectrometry-based proteomics methods for making protein folding stability measurements on the proteomic scale have been reported over the past decade, none have been interfaced with top-down proteomics. Described here is a top-down (TD) stability of proteins from the rates of oxidation (SPROX) approach for making proteoform specific folding stability measurements. This approach is validated using a mixture of three model proteins with well-characterized protein folding behavior by conventional SPROX as well as other more conventional biophysical techniques. The method is also used to evaluate the relative folding stabilities of the <30 kDa protein fraction isolated from an MCF-7 cell lysate. The relative folding stabilities of 150 proteoforms from 83 proteins were successfully characterized in the cell lysate analysis using the TD-SPROX approach.

doi.org

Is “proteomics approach” the same thing as “proteomic approach”? I was under the impression that you should always add the s at the end of -omics, even as an adjective because it’s part of the word to show that we’re measuring thousands of targets at once. But I see people using proteomic without s.

Going home from the lab be like 🥶. First snow is Chicago!