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Johana Misas Villamil

@jomivi.bsky.social

Scientist, mother of two, love vacations and proteases 🇨🇴

35 Posts184 Follower128 Folgt
Dan Garisto@dangaristo.bsky.social · 5d ago

In May it was big news when 100+ suspicious images were found in Thermo Fisher's catalog. Now, that number has expanded dramatically to 18,000+, thanks to some arduous searching by @reeserichardson.bsky.social. My reporting on the massive trove and its implications for antibody reliability:

More than 18,000 questionable images found in antibody catalogues of 15 companies

Science sleuth’s findings raise questions for researchers working to improve the reliability of commercial antibodies.

nature.com

Jack Rhodes@jackrhodes.bsky.social · 2w ago

Diverse phytocytokines derived from conserved subtilase scaffolds co-evolved with clustered receptors to confer defence responses in legumes www.nature.com/articles/s41...

Diverse phytocytokines derived from conserved subtilase scaffolds co-evolved with clustered receptors to confer defence responses in legumes - Nature Plants

This study identifies SubPEP variants in soybean that confer tissue-specific immunity via GmSubPEP Receptors. Their precursor genes and receptor genes are colocalized in genomic clusters, revealing th...

nature.com

Lorenz Oberkofler@oberkoflerlorenz.bsky.social · 3w ago

Super happy to finally share our preprint with the community! 🎉 Fungal extracellular vesicles deliver RNA cargo into host cells - but how do they get through the plant cell wall? This question puzzled us and the field for years… 🧵 1/6

Maurice König@maukoe.bsky.social · 3w ago

Very happy to share our new paper in Plant Physiology! 🌽 We uncover a 2-step mechanism controlling maize phytocytokine Zip1: ZmMC9 processes PROZIP1 intracellularly, enabling export of bioactive Ct-PROZIP1, while apoplastic proteases attenuate the signal. doi.org/10.1093/plph...

Schematic model of the two-step processing and regulation of the maize phytocytokine PROZIP1/Zip1. Intracellularly, PROZIP1 associates with the ER and is cleaved by the Ca²⁺-dependent type II metacaspase ZmMC9, generating the C-terminal fragment Ct-PROZIP1. This bioactive fragment is exported to the apoplast through a Golgi-independent route, where it activates immune signaling. In a second step, apoplastic proteases, including the papain-like cysteine proteases CP1 and CP2, further process and degrade Ct-PROZIP1 and Zip1, thereby attenuating and clearing the signal.
Ryohei Thomas Nakano (Hokudai)@luckystrike1984.bsky.social · last mo.

So excited to share our new study about nicotine-mediated tobacco root-microbiota interactions, spearheaded by Tomohisa! Nicotine is a neurotoxin for insects, but for Arthrobacter, it works as a nutritional source that provides a competitive advantage. doi.org/10.1186/s401...

Horizontal acquisition of nicotine catabolism gene cluster enhances Arthrobacter fitness within tobacco root microbiota - Microbiome

Background Plant roots are hotspots for interactions with soil microbes, where a characteristic bacterial community structure is formed. Plant specialized metabolites often play pivotal roles in this assembly process. However, the molecular basis underlying root microbiota responses to these bioactive compounds, and how such metabolic interactions shape the assembly of host-specific root microbiota, remain largely unknown. Nicotine is a toxic alkaloid predominantly produced by the genus Nicotiana, and the genus Arthrobacter is known as one of the nicotine-degrading bacteria in the tobacco root microbiota. In this study, we used the tobacco–Arthrobacter interaction system as a model and integrated comparative genomics and experimental genetic manipulation assays to uncover the role of bacterial catabolism capacity for host specialized metabolites in shaping host-specific root microbiota. Results Nicotine catabolism genes are uniquely found in the Arthrobacter strains derived from nicotine-containing environments, and this restricted gene distribution is driven by a plasmid-mediated horizontal gene transfer. To assess the ecological consequences of this genomic adaptation in Arthrobacter fitness in tobacco roots, we characterized the nicotine utilization ability of Arthrobacter and conducted adaptation assays under in planta conditions using genetically manipulated Arthrobacter strains and tobacco mutants impaired in nicotine catabolism and biosynthesis, respectively. Nicotine improves Arthrobacter colonization of tobacco roots through a catabolism-dependent mechanism. Bacterial community analysis using a synthetic community approach further demonstrated that this metabolic adaptation enhances Arthrobacter fitness within tobacco root microbiota. Conclusions Our findings illustrated that bacterial catabolic capacity toward host-derived plant specialized metabolites is key for successful root colonization. This metabolic adaptation is driven by plasmid-mediated horizontal gene transfer and ultimately shapes the structure of the root microbiota community. Video Abstract

doi.org

Tonni Andersen@tonnigrubeandersen.bsky.social · 2mo ago

Time for another preprint from the lab: www.biorxiv.org/content/10.6... Led by @yuanyuanzhang.bsky.social and @defengshen.bsky.social. We found some surprising secrets that connect and uncouple root barrier formation with systemic nitrogen signaling in shoots. see the thread below for more details

A tunable receptor separates root barrier formation from nutrient signaling through ligand-perception states

In roots, the endodermis controls nutrient entry by forming a barrier known as the Casparian strip. Yet how barrier-associated processes interface with systemic signaling remains unclear. Here, we sho...

biorxiv.org

Pedro Beltrao@pedrobeltrao.bsky.social · 2mo ago

My department @eth-dbiol.bsky.social at ETH Zurich is hiring for a tenured professor in mol cell bio and biochemistry (associate/full). The position is within the Institute of Biochemistry (www.bc.biol.ethz.ch) and it is fairly open within their scope of research. www.nature.com/naturecareer...

Professor of Molecular Cell Biology and Biochemistry - Zurich, Switzerland job with ETH Zurich | 12861386

The Department of Biology at ETH Zurich invites applications for the above-mentioned tenured professorship at the Institute of Biochemistry

nature.com

Plant Science Spotlight@plant-sci.bsky.social · 2mo ago

🍅🧬Cold induced peptides SlRGF9 and SlRGF10 protect tomato pollen. Upregulation prevents cold induced yield loss by up to 52 percent. The pathway is conserved in rice, recovering 18 percent of grain yield loss. A core cold resilience axis for crop protection #plantsci www.nature.com/articles/s41...

Cold-induced peptide signalling secures pollen resilience and crop yield - Nature

A small-peptide signalling axis involving RGF family members controls resilience to cold stress in tomatoes and rice; modulating this pathway prevents cold-induced yield losses.

nature.com

Lin-Jie Shu@linjieshu.bsky.social · 3mo ago

The first fatty acid-binding structure in a “G-type lectin domain” among plant PRRs has been verified! A new LORE paper is out from Ranf lab at TUM in Germany and University of Fribourg in Switzerland and @antonelladipizio.bsky.social at TUM : doi.org/10.64898/202... Here are some cool highlights:

The plant immune receptor LORE binds agonistic and antagonistic 3-hydroxy fatty acid ligands via a dynamic loop in its G-type lectin domain

The Arabidopsis thaliana S-domain receptor kinase LORE senses bacterial medium-chain 3-hydroxy fatty acids (mc-3-OH-FAs) as microbe-associated molecular patterns to activate pattern-triggered immunity...

doi.org

The Sainsbury Laboratory@thesainsburylab.bsky.social · 3mo ago

TSL Synbio just deposited 192 highly requested plasmids on @addgene.bsky.social! 🧪 Popular by demand: Our deposits include MoClo Plant promoters, plant terminators, C-terminal and N-terminal tags. Find them here: www.addgene.org/kits/youles-...

Addgene: Plant MoClo Tags, Promoters, Terminators, and Acceptors

A MoClo collection containing a selection of N-Terminal and C-Terminal tag modules, common plant promoters and terminators, and acceptor vectors.

addgene.org