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Baayla Boon

@baaylab.bsky.social

7 Posts93 Follower46 Folgt
Danielle Beckman@daniellebeckman.bsky.social · 12mo ago

What can you do to improve your microscopy images? I often receive questions regarding how to take better images, so let’s start with the basics: improve your sample preparation! No microscope can make a miracle if your samples don’t look great! Things I don’t do anymore: (1/5)

Replace paraffin embedding for frozen tissue sectioning: The process of fixation, dehydration, and infiltration with paraffin can damage or destroy some crucial antigens. Avoid working with mounted slide and use free-floating sections: Allows antibodies and other reagents to penetrate the tissue from all sides,  especially when combined with overnight incubation in a shaker. Thin vs. thick sections : What do you want to analyze? Thicker sections will allow more complex 3D reconstructions and blood vessel analysis. DAB vs. immunofluorescence (IF): IF will produce less background and allows studying co-localization of multiple targets
Baayla Boon@baaylab.bsky.social · 2y ago

By going beyond ‘Atypical’ / ‘AD’ @jeffandthebrain.bsky.social got me excited about interesting unexplored avenues. And! A whole lot of relevant papers I still need to read. Check the recording @istaart.bsky.social available in ~3 weeks if you missed it!

Jeff Phillips@jeffandthebrain.bsky.social · 2y ago

Starting shortly--please join us to review key research from 2024 on atypical Alzheimer's disease, selective vulnerability, anti-amyloid therapies, and other topics of interest!