Tessera

Joram Dongus

@drdongus.bsky.social

Researcher Gene-editing at Wageningen University Creating a ThermoCas9 gene editing toolkit for major monocot and dicot crops

2 Posts48 Follower 50 Folgt

Joram Dongus @drdongus.bsky.social · 4mo ago

New bioRxiv preprint!🌱 We found the SAUERKRAUT transposon to regulate the floral transition in a salt-dependent manner!🧂 A step toward salt-tolerant crops in a changing climate!🌞🥬 🔗 doi.org/10.64898/202... #PlantScience #SaltStress #ClimateChange @pph-wur.bsky.social @christatesterink.bsky.social

Salt stress alters plant development, including the floral transition, but regulation of timing of flowering by salt is poorly understood at the molecular level. To identify genetic loci regulating the floral transition under high soil salinity, we performed a genome-wide association study (GWAS) in Arabidopsis thaliana and identified natural variation at the UGT74E1-UGT74E2-BT3 (UUB) locus that correlates with bolting time specifically in response to salt stress. Genetic analysis revealed BT3 as a novel repressor of the floral transition in control conditions. Similarly, the putative IBA glycosylases UGT74E1 & UGT74E2 delay the floral transition in control conditions. Furthermore, we identified that IBA homeostasis regulators TOB1 and ECH2/IBR10 play a key role in the floral transition, and that ECH2/IBR10 are required for the early flowering phenotype of the ugt74e1/ugt74e2 double mutant, indicating that UGT74E1 & UGT74E2 delay flowering by altering IBA homeostasis. A pangenome analysis of the UUB locus revealed variation in the occurrence of the DNA transposon SAUERKRAUT (SKRT). CRISPR-mediated SKRT deletion in Col-0 affected gene expression both within and outside the UUB locus and caused a salt-dependent delayed floral transition. The delayed bolting phenotype of the skrt-2 mutant also depends on ECH2/IBR10 function, indicating that SKRT accelerates the floral transition by altering IBA homeostasis. Finally, targeted demethylation of SKRT resulted in delayed floral transition under salt stress. Taken together, our data show a role for SKRT and its DNA methylation levels in the salt-dependent bolting time response in Arabidopsis, revealing a novel molecular mechanism to control flowering in adverse conditions.

Nick Desnoyer @nickdesnoyer.bsky.social · 2y ago

In one year, I genetically engineered the boring Arabidopsis into a beautiful ornamental flower 🧬🌹 Here is how and why I gave this model organism a visual upgrade 🧵(1/7)

Resource @resourcewur.bsky.social · 2y ago

Ingezonden brief: ‘Het bestuur moet fouten erkennen’ ‘WUR bracht met de opruiming van de brug geweld terwijl vrede werd geëist’, schrijft onderzoeker Fernando in een ingezonen brief. www.resource-online.nl/index.php/20...

Ingezonden brief: 'Het bestuur moet fouten erkennen' - Resource online

Het bestuur bracht met de opruiming van de brug geweld terwijl vrede werd geëist, schrijft onderzoeker Fernando in een ingezonen brief.

resource-online.nl

Marc Somssich @somssich.bsky.social · 2y ago

I never had a use for this image in any research paper, so eventually I used it in my short history of plant light microscopy, as it is one of very few examples of @henriqueslab.bsky.social & @superresolusian.bsky.social's #SRRF-microscopy applied in plant cells. #PlantScience #PlantMicroscopy 👇

A Short History of Plant Light Microscopy

When the microscope was first introduced to scientists in the 17th century, it started a revolution. Suddenly, a whole new world, invisible to the naked eye, was opened to curious explorers. In respo...

currentprotocols.onlinelibrary.wiley.com

Parvinder Kahlon @parvinderkahlon.bsky.social · 2y ago

New Preprint! Excited to share our method for profiling light elements in plant tissues using XRF. Fast, cost-effective, and no extraction needed—perfect for stress-related studies in plants. Read more: www.biorxiv.org/content/10.1... #PlantScience #XRF #Innovation

Quantitative light element (sodium and potassium) profiling in plant tissues using monochromatic X-ray fluorescence analysis

Accurately determining the elemental composition of plant tissues is essential for physiological studies on plant stress, including salinity tolerance. However, high-throughput routine analysis of lig...

biorxiv.org