I am so full of cool ideas for the channel and the lab! Thank you #OSPARK organizers @digiresacademy.bsky.social and participants! There were two amazing days of learning and interacting for a common goal: promote #openscience and #FAIR principles.

Does anyone located in Europe can share with us the Bacillus subtilis strains 168 or the B. Subtilis WB600 and their expression vectors? 🧫 🙏 If you do, please send me a message so I can contact you for the details. Thank you! 🧪 #microbiology #protein_expression

My favorite tool #Opencloning.org has just released a feature for assembling golden gate cloning fragments. I have not used it **yet**, but I've used the In-Fusion tool, and that ones has worked amazingly. Give it a try! I am sure @manu-lera.bsky.social will be happy to hear from your experience.

I got a plasmid map from another lab as *.dna file 🧪. The SnapGene program can open it. I do not want to install their "free" viewer in my linux system. The solution: OpenCloning opencloning.org . With it, I loaded the .dna file and downloaded the sequence plus a nice map. Thanks OpenCloning team 🤩

Finally 🥳. Our resource paper under Open Science and FAIR data principles 🧪. I never thought that a simple resource paper will take more than 1 1/2 years to get reviewed. But now it is finally out bmcresnotes.biomedcentral.com/articles/10....

Toxin data quality: a critical examination of bacterial exotoxins and animal toxins - BMC Research Notes

Objective Existing toxins datasets include a mixture of proteins and toxin peptides. In this study we present two curated datasets of toxic proteins free of associated proteins: bacterial exotoxins an...

bmcresnotes.biomedcentral.com

🧪Our first paper with Machine Learning under Open Science principles and FAIR data is out! rdcu.be/ezXFx

You have analized your data with many steps. Do you want to make the analysis reproducible for others and your future-you? If you are in the LMU Munich, you can come to our workshop on *May 6th*. We will show you how to create workflows in R. Www.pretix.osc.lmu.de/lmu-osim/hig... 🧪

Reproducible analysis of high-throughput data with R

Tue, May 6th, 2025

pretix.osc.lmu.de

More of this, please. Welcome to Bluesky, finally crawling out of the Upside Down? No Demogorgons here, the forecast is clear, the science sharper, and the community healthier. www.reuters.com/world/europe...

EU medicines agency quits X, moves to Bluesky

The European Medicines Agency (EMA) said on Monday it would no longer post on X and would use rival Bluesky instead, becoming the latest organisation to quit a social media platform that some have criticised for its content.

reuters.com

« Assessing the feasibility and impact of clinical trial trustworthiness checks via an application to Cochrane Reviews: Stage 2 of the INSPECT-SR project » by a gang of researchers led by @jackwilkinson.bsky.social

Assessing the feasibility and impact of clinical trial trustworthiness checks via an application to Cochrane Reviews: Stage 2 of the INSPECT-SR project

Background The aim of the INSPECT-SR project is to develop a tool to identify problematic RCTs in systematic reviews. In Stage 1 of the project, a list of potential trustworthiness checks was created....

medrxiv.org

Iron is essential for growth of Tetrahymena in axenic media (prey-free)🧪. Last time we made our stock solution was three years ago. How do we prepare it and store it? The details are now published in @protocolsio.bsky.social under www.protocols.io/view/fecl3-s... and just in time for a new lot 🥼🫡

FeCl3 solution for NEFF Media

For axenic growth of Tetrahymena thermophila in the lab, NEFF media requires a final concentration of 33 µM (see Tetrahymena Stock Center (RRID:SCR_008362)). To guarantee that...

protocols.io

Buffer is ready. Hmm. can I sterilize it in the autoclave? When the answer is no, the next best option is to do a sterile filtration. It's not a big deal, but it's easy to do it wrong. Here is our protocol! 🧪 Sterile filtration of liquid solutions  www.protocols.io/view/sterile...

Nothing more annoying than preparing buffers, but extremely necessary if we want to mate ciliates. Here is our protocol for the 1 M Tris-HCl pH 7.5 for Tetrahymena mating (Stock solution) www.protocols.io/view/1-m-tri.... We hope it will be helpful for the community! 🧪 🤲🏻🥼

1 M Tris-HCl pH 7.5 for Tetrahymena mating (Stock solution)

In this protocol we specify the steps needed to create a 250 ml stock solution of 1M Tris-HCl solution used for the preparation of Tetrahymena thermophila cells for mating. The...

protocols.io

I love to help to solve challenges that come up in the lab, and yesterday I was able to link our protocol for veggie NEFF media in a personal communication (www.protocols.io/view/veggie-...) while discussing differences in flow cytometry results. Life is easier with @protocolsio.bsky.social 🤪🧪

🧪⚛️😂🤣

Uncle Duke@uncleduke1969.bsky.social · 2y ago

i'm no stephen hawking but i think what happens is that they cancel each other out

We still have places in our Machine Learning School. Anyone in natural sciences or medical background can take part. You just need to be in Munich between the 9th and 12th of December and have the full day available! You will learn the basic of python and will apply it to create your own ML model 🧪

Two more protocols out in @protocolsio.bsky.social. Little by little we can build upon each one to get everything ready for the big ones. This time we have a buffer solution for Quality Control of the AlamarBlue stain, and a classic: Gram staining www.protocols.io/view/gram-st....

Question to the community 🧪: in the last 4 years, I have tutored PhD students from other labs and institutions. Too many have had a lot of problems because of bad PIs, and I do mean really bad scientist and people. Some are under investigation. How can I warn future students about them?

First-of-its-kind Online marketplace for sharing tissues, organs and other biological materials. @science.org By @arukon0.bsky.social #scisky #news #science www.science.org/content/arti...

Online marketplace for animal samples could cut waste and save lives

First-of-its-kind platform will allow scientists to share tissues, organs, and other biological materials

science.org

These are outstanding resources for anyone engaged in creating scientific knowledge. 🧪🛟🩺

Science News@scinews.bsky.social · 2y ago

Three must read papers for PhD students. #scisky #PhD #science #research #academicsky 1. The importance of stupidity in scientific research Open Access journals.biologists.com/jcs/article/...

www.linkedin.com/posts/eric-p... Really interesting view... how would you measure trustworthiness? 🧪

Eric Partaker on LinkedIn: It's a HUGE mistake to promote a toxic manager. → And it starts by… | 475 comments

It's a HUGE mistake to promote a toxic manager. → And it starts by ignoring poor behavior. As Justin Wright recently share with this post, a toxic manager is… | 475 comments on LinkedIn

linkedin.com

At erc.europa.eu/news-events/..., they link a document with the list of criteria on how to evaluate if a data repository complies with the new Horizon Europe grants. A list of trusted repositories should be in the document, too 🧪. Make you data FAIR!

New guide on trusted repositories: Promoting open science and meeting grant requirements

A new study provides a list of trusted repositories across different fields of research, with an assessment of their readiness to facilitate compliance with the Horizon Europe requirements.

erc.europa.eu

www.linkedin.com/posts/ivan-k...

The plan is to make all our protocols available for the community. Thanks to my team and their hard work, the first one is out! www.protocols.io/view/tb-medi.... Thanks to @protocolsio.bsky.social for such a great platform. 🧪

Do you want to start to see your data from the point of view of Machine Learning? Look into our course description under mlschool.rostlab.org and come if you are interested. We have places open and it is free! Our focus is biological sciences 🧪

Curious about how you can use your data in the 21st Century? Come and learn with us between the 9th and 12th of December about Machine Learning (mlschool.rostlab.org). After you learn the basics of python, we dive into tidy (FAIR) data, and your first model under best practices in the field. Free!!!

The fun starts now! Medical students are coming out, and we are ready for them!

Tod@s podemos ayudar a transformar ciencia a Ciencia Abierta! Publica tus protocolos de laboratorio en detalle en @protocolsio.bsky.social. Una vez publicados se pueden referenciar con el DOI! Así es mas fácil explicar métodos en tus publicaciones y tesis! Incrementa tu visibilidad! 🧪

For everyone in Munich or with an option to stay in the area in December: we have still places in our Winterschool for Toxicologist -Machine learning version (December9th to 12th). Check it out... and it is free. Mlschool.rostlab.org

And yes, we did just add "share on @bsky.app" to @protocolsio.bsky.social just as @altmetric.com recommended bsky.app/profile/altm.... (Thank you for the nudge @exotoxinslab.bsky.social!)

Altmetric@altmetric.com · 2y ago

We strongly suggest that academic publishers and other platforms that host research rapidly implement a Share to Bluesky button for their articles. Here's how: docs.bsky.app/docs/advance... #AcademicSky #HigherEd #Altmetrics

You can do it too! Start promoting Open Science by publishing detailed protocols with the help of @protocolsio.bsky.social. Very happy to help with the visibility of protocols made available by other communities! Veggie NEFF media for Tetrahymena thermophila www.protocols.io/view/veggie-...