Preparing another Nanopore/Genome assembly workshop for tomorrow to round off #ICOM2026 in style. Drew me back to @protocolsio.bsky.social. We started over 10 years ago with long-reads, with 10+ workshops and lots of smiles. Memory lane made me smile. 1/2

"RSV-A and RSV-B Amplicon Sequencing from Solid-Phase Wastewater Using the NEBNext® RSV Primer Module" is a novel protocol by CIDMATH researchers Said Rachida, Alaa Ahmed, Hannah Cayla D., Abby Paulos, Orlando Sablon, Marlene Wolfe, and Anne Piantadosi.

🧬 We’ve shared an open-access protocol for cost-effective, high-quality DNA extraction from cyanobacteria and microalgae. We hope it will be useful to the research community! Developed with @Raul_MontanezM. doi.org/10.17504/pro... #Cyanobacteria #Microalgae #DNAExtraction #Genomics #ProtocolsIO

Lab protocol: DNA extraction from cyanobacteria and microalgae

Lab protocol: DNA extraction from cyanobacteria and microalgae. Photosynthetic microorganisms are key. Read full protocol, steps, and materials on protocols.io

doi.org

Working with faba bean (Vicia faba)? We've published a rapid, reliable DNA extraction protocol that avoids the rapid blackening of tissues, a common headache during DNA isolation. Protocol: www.protocols.io/view/rapid-d... Feedback and experiences are welcome! #PlantSci #FabaBean #OpenScience

Rapid DNA extraction from faba bean (Vicia faba) tissues

Rapid DNA extraction from faba bean (Vicia faba) tissues. Efficient DNA isolation is essential for hig. Read full protocol, steps, and materials on protocols.io

protocols.io

🧵 New paper! 🚨 Delighted to see our GETCampy-Africa protocol published in BMJ Open. *Protocols for genomic epidemiology and source attribution of enteric bacteria causing diarrhoeal disease across Africa* @bmj.com @bmjmedicine.bsky.social 📄 bmjopen.bmj.com/content/16/6... 👇

🧠🧬🧪🔬🧫 🥼Toronto science friends: I have a highly-motivated research student, Ashlee Lewis, who is looking for research-related opportunities. She recently graduated with a B.S. in Biochemistry, and has excellent wet-lab, computational (R), and communication skills. www.linkedin.com/in/ashleelew...

We’re transforming research protocols from static documents into dynamic, reusable workflows. A key part is making large collections of trusted methods — including Protocol Exchange and Springer Protocols — available in protocols.io format, so researchers can reuse, adapt, and build on them.

Evolving protocols: From static to dynamic with protocols.io

Sharing research protocols is a cornerstone of open science, and protocols.io, part of Springer Nature, continues to evolve as a secure, modern platform for...

protocols.io

With extensive step by step protocols detailed here www.protocols.io/view/collect...

delighted to share our new pre-print developing label-free glycosaminoglycan analysis of diverse complex biological samples, led by @h-davies-s.bsky.social working closely with David Knight and Rebecca Miller alongisde our recent recruit Samie Dalgarno www.biorxiv.org/content/10.6...

www.protocols.io/view/cryo-ex... New protocol alert!! Cryo-expansion microscopy of C. elegans and Tardigrades using the high pressure freezer!

Cryo-Expansion Microscopy of C. elegans and Tardigrades

Cryo-Expansion Microscopy of C. elegans and Tardigrades. Expansion microscopy (ExM) improves imaging r. Read full protocol, steps, and materials on protocols.io

protocols.io

Attending ABRF 2026 (@abrf.bsky.social)? Connect with protocols.io at Booth 710. We're engaging with core facilities about common priorities and challenges, including how to demonstrate impact and receive credit. If this resonates, schedule a short discussion with our team: abrf26.youcanbook.me

Join our webinar to hear from researchers at the University of Rochester and City of Hope on practical strategies for improving documentation, supporting training, and increasing visibility of core facility work. April 9 | 11 AM EST | 5 PM CET Register here: go.springernature.com/enhancing-im...

Last month, we highlighted Protocols.io as a new resource available at @bodleian.ox.ac.uk. Next week, as part OxFOS, you can sign up and join the Springer Nature team for a training session introducing the platform to researchers either in person here at the RSL or online go.glam.ox.ac.uk/proto

Started this project years ago, but now everything is deposited on @addgene.bsky.social and a short @protocolsio.bsky.social is online. Try it out to make your own (cheap) homemade nuclease/Benzonase! dx.doi.org/10.17504/protocols.io.261ged7xov47/v2 Thank you @jcoker10.bsky.social and Michael Lim!

We've updated our protocols.io page to highlight a couple of the protocols published by Crick authors recently. If you're at the Crick, don't forget you can get your flat pdfs converted to the protocols.io format at no cost! 😀 www.protocols.io/organization...

ONT seq help: Has anyone had success using less than the 10ng of input DNA for the 16S barcoding kit? Trying to use it for low biomass samples.

a woman in a pink dress sits on a green pole on a stage

ALT: a woman in a pink dress sits on a green pole on a stage

media.tenor.com

Given the recent #Nipah outbreak in India, here are two #Nanopore protocols for NiV genomic sequencing in support of rapid outbreak response: IPC: www.protocols.io/view/sequenc... icddr,b/CDC: journals.asm.org/doi/10.1128/...

New @protocolsio.bsky.social post describing our new protocol for generating intestinal organoids for #stickleback: www.protocols.io/view/generat... With work by postdoc Arshad Padhiar, funded by @moorefound.bsky.social

Generation of Stickleback intestinal organoid

Intestinal organoids offer significant advantages over conventional 2D cultures, providing a physiologically relevant 3D architecture that enables more realistic disease modelin...

protocols.io

Join Springer Nature editors and the protocols.io team for an exciting event as part of #LoveMethods26! 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22. Our editor Sabrya Carim will be there! 👉FREE registration & more details: www.protocols.io/blog/join-pr...

Bring structure to your research - protocols.io

A secure platform for developing and sharing reproducible methods.

protocols.io

Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26! 💻Transform your protocols at our Hackathon on Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22 👉FREE registration & more details:

Join protocols.io and Springer Nature editors at Love Methods Week 2026

Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...

protocols.io

You want to take sediment samples from archaeological sites for a variety of analysis?Have a look at our best practise sampling protocol: www.protocols.io/view/samplin...

Sampling protocol for obtaining sediment from archaeological excavations in caves and rockshelters for the ...

The objective of this protocol is to guide systematic collection of sediment samples from archaeological excavations in caves and rockshelters in order to perform biomolecular a...

protocols.io

Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs at Love Methods Week 2026excelscior.uc.pt/love-methods...6/ #LoveMethods26

Some EBP-affiliates are embarking on ambitious R&D to tackle some of the hardest genomes on Earth. 🌍🧬 Great science starts with shared ideas — sometimes over coffee. 💥🤝☕️ Soak up the Sanger Tree of Life sponge protocol 👇 www.protocols.io/view/sanger-... @seagrassliz.bsky.social #Biodiversity

A new Lab Protocol from @sabass-lab.bsky.social and colleagues presents an in vitro rumen culture and workflow that reproducibly screens anti-methanogenic compounds, aiding feed-based methane mitigation studies: plos.io/4oyyc4a Protocol available via @protocolsio.bsky.social at plos.io/3KAOThi

A reliable in vitro rumen culture system and workflow for screening anti-methanogenic compounds

Arguably the biggest man-made challenge of the century is halting climate change. Livestock’s methane (CH4) emissions, a greenhouse gas with a higher global warming potential than carbon dioxide…

plos.io

Exciting news: Premium protocols.io is now available via Research4Life to researchers in low- & middle-income countries—at no or low cost. 🌟 Full Springer Nature press release: group.springernature.com/gp/group/med... #OpenScience #Reproducibility #GlobalCollaboration #Research4Life #protocolsio

It was great to hear from @lteytelman.bsky.social about Protocols.io today for our LIBRE hub channel on open science! We already have a tutorial in Spanish: youtu.be/RuBKWouEyL8?...

Construyendo protocolos científicos reproducibles y abiertos con Protocols.io

YouTube video by LIBRE hub

youtu.be

We have done our first @protocolsio.bsky.social. Are you interested about #genome_annotation quality evaluation? We have developed a tool called #GAQET2 (github.com/victorgcb198...) which runs several tools like #AGAT, #BUSCO, and many other tools. You can find it at www.protocols.io/view/structu...

Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub librehub.github.io) is an amazing initiative from @makertobey.bsky.social and colleagues!

The Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub)

...

librehub.github.io

With the full CRISPR 7-11 protocol here :)

@sofiram.bsky.social · 9mo ago

Link for the full CRISPR Cas7-11 protocol: www.protocols.io/view/crispr-...

Link for the full CRISPR Cas7-11 protocol: www.protocols.io/view/crispr-...

CRISPR/Cas7-11 tool for RNA targeting in Xenopus laevis

CRISPR/Cas systems are defense mechanisms in prokaryotic cells to induce the cleavage and degradation of nucleic acids derived from bacteriophages. Among the different classes,...

protocols.io