5 more years of funding! Thanks NIGMS! If you are interested in pursuing a postdoc in the Galligan Lab, please reach out!
Galligan Lab
@galliganlab.bsky.social
University of Arizona. Studying all things PTM and metabolism. Galliganlab.com
How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid? Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7) www.nature.com/articles/s41...
Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry
Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...
nature.com
Can a cocktail of glycation-lowering compounds curb #hunger, lower #insulin resistance and extend #lifespan? It does in mice! Buck scientists fight the sugary chemistry of #aging. www.buckinstitute.org/news/glycati... @cp-cellreports.bsky.social, @pkapahi.bsky.social
Glycation-lowering compounds curb hunger, lower insulin resistance and extend lifespan in mice
Buck scientists fight the sugary chemistry of aging The same chemical reaction that makes a piece of freshly toasted bread delicious also happens in our bodies, with far less appetizing consequences. ...
buckinstitute.org
A new paper used reactivity-based metabolomics to show that methylglyoxal and its main metabolite lactoylglutathione react rapidly with cysteines to generate L- and D-lactoylated thioesters suggesting an additional pathway to enable protein lactoylation www.nature.com/articles/s41...
Reactivity-based metabolomics reveal cysteine has glyoxalase 1-like and glyoxalase 2-like activities - Nature Chemical Biology
Using reactivity-based metabolomics, methylglyoxal and its main metabolite lactoylglutathione were found to react rapidly with cysteines to generate l-lactoylated and d-lactoylated thioesters, suggest...
nature.com
Super excited to share our new publication identifying the food preservative, sorbic acid, as a new PTM. Even cooler is that the deacylation and acylation of this PTM is catalyzed by the non-canonical activity of Class I HDACs using free sorbate (not sorbyl-CoA) www.science.org/doi/10.1126/...
Sorbate induces lysine sorbylation through noncanonical activities of class I HDACs to regulate the expression of inflammation genes
Sorbic acid regulates inflammatory gene expression through the mechanism of lysine sorbylation.
science.org
www.nature.com/articles/s41...
Reactivity-based metabolomics reveal cysteine has glyoxalase 1-like and glyoxalase 2-like activities - Nature Chemical Biology
Using reactivity-based metabolomics, methylglyoxal and its main metabolite lactoylglutathione were found to react rapidly with cysteines to generate l-lactoylated and d-lactoylated thioesters, suggest...
nature.com
Excited to share this one! We developed an in vivo model for specific manipulation of transfer RNA acetylation and found it serves as a sentinel modification whose loss causes ribosome stalling and stress signaling. Implications for a genetic disorder and cancer. www.science.org/doi/10.1126/...
Transfer RNA acetylation regulates in vivo mammalian stress signaling
An ancient tRNA modification is used by mammalian cells to coordinate protein translation and adaptive signaling.
science.org
I am extremely proud to see our work about Hepatitis B now published in @cellpress.bsky.social From basic research to identifying the key step of infection & a potential first drug for curing this devastating disease. This has been an incredible journey.🧵 www.cell.com/cell/fulltex...
A nucleosome switch primes hepatitis B virus infection
A reconstituted cccDNA platform facilitated the discovery of an early chromatinization event that promotes transcription of the hepatitis B virus (HBV) regulatory X gene. The use of a small-molecule c...
cell.com
First post! Starting by pinning our review in Nature Metabolism on Coenzyme A biosynthesis and metabolism. Check it out if you didn't catch it when it was published! www.nature.com/articles/s42...
Coenzyme A biosynthesis: mechanisms of regulation, function and disease - Nature Metabolism
This Review summarizes the fundamental aspects related to coenzyme A synthesis and its implications as a central molecule in metabolism.
nature.com
Happy New Year. I’m pleased to report publication of the Ducker lab’s latest cancer metabolism paper led by graduate student Kyle Dunlap in @cp-cellreports.bsky.social www.cell.com/cell-reports.... 1/n
SLC7A5 is required for cancer cell growth under arginine-limited conditions
Metabolic adaptations are key to cancer cell survival in nutrient-limited microenvironments, but strategies for coping with arginine scarcity are poorly understood. Dunlap et al. use CRISPR screening and metabolomics to identify the plasma membrane transporter SLC7A5 as a citrulline transporter essential for cancer cell growth under arginine limitation.
cell.com