Our first mass spectrometry based single cell proteomics dataset is opnely available. Explore protein abundance profiles across all tumour associated neutrophil populations. And thanks to @wellcometrust.bsky.social we have funding to develop new visualisations and modules for SCP. #immunosky

Alejandro Brenes@ajbrenes.com · 8mo ago

Excited to share our work at @natcomms.nature.com We used single cell proteomics to define the functional heterogeneity of human neutrophils in glioblastoma, finding pro and anti-tumorigenic effector states invisible to scRNAseq. SCP will revolutionise immunology, this is just the start

Excited to share our work at @natcomms.nature.com We used single cell proteomics to define the functional heterogeneity of human neutrophils in glioblastoma, finding pro and anti-tumorigenic effector states invisible to scRNAseq. SCP will revolutionise immunology, this is just the start

Alejandro Brenes@ajbrenes.com · last yr.

Happy to share our latest preprint doing low cell number (mini-bulk) and single cell #proteomics on tumour associated neutrophils from human glioblastoma where we find multiple functional states that would be invisible to scRNAseq, some showing pro-tumoural states with potential therapeutic value

I am overjoyed to present our latest paper on Autophagy in CD8 Tcells! www.nature.com/articles/s41... It was, as all ‘sciencing’ is, a huge team effort. I will try to do it some justice in the following thread 😊 1/n @cantrell-lab.bsky.social @immpres.bsky.social @ajmhowden.bsky.social

Autophagy repression by antigen and cytokines shapes mitochondrial, migration and effector machinery in CD8 T cells - Nature Immunology

Sinclair et al. combine quantitative proteomics and an autophagy flux reporter to map autophagy substrates and triggers during CD8 T cell differentiation. Proteins degraded and fueled by autophagy in ...

nature.com

We have new exciting data coming your way soon. New murine T cell and B cell datasets. Keep tuned! This means ImmPRes will have included 23 murine proteomic datasets!

Data is on ImmPRes @immpres.bsky.social and full paper on @elife.bsky.social elifesciences.org/articles/53725 This was an old dataset so it is DDA based LFQ with 8 fractions. Coverage was good but it has the normal issues of label free DDA

Quantitative analysis of how Myc controls T cell proteomes and metabolic pathways during T cell acti...

Myc-dependent induction of amino acid transporter expression in response to T cell receptor activation is essential to enable T cell proteome remodelling upon immune activation.

elifesciences.org

📢 we have a schedule maintenance. ImmPRes will be down on the 27th and expect to be back online by the 29th.

🧪🧪 The Immunological Proteome Resource is now on Bluesky as well. ImmPRes is an open access resource created to provide an in-depth, high quality, quantitative map of the immuno proteome. It integrates immune cell proteomic data generated by quantitative mass-spectrometry. #immunology #proteomics