Linda Sinclair
@lindavsinclair.bsky.social
Immunologist, working with Cantrell Lab. Interested in LOTS - focused on nutrient and metabolic control of the immune system. (opinions are my own)
I have a 3-year postdoc position available at University of Manchester for a mass spec expert in proteomics. The person will be involved in exciting projects in single cell proteomics, drug discovery and innate immunity - on timsTOF Ultra AIP & HF & Astral Zoom. www.jobs.ac.uk/job/DQJ093/r...
Home - Trost Lab
trostlab.org
****2-NBDG does not measure glucose uptake**** Just your regular reminder of this fact 😁. See the rigorous proof of this fact in @lindavsinclair.bsky.social 2020 paper. This paper was 6 years ago!! Stop using 2NBDG to measure glucose uptake already 🙄 pmc.ncbi.nlm.nih.gov/articles/PMC...
Single Cell Glucose Uptake Assays: A Cautionary Tale
Assays to monitor the metabolic state or nutrient uptake capacity of immune cells at a single cell level are increasingly in demand. One assay, used by many immunologists, employs 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose ...
pmc.ncbi.nlm.nih.gov
2 new efforts: from Megan Teh on metabolism of CD8 Tcells in iron deficiency and why they love aspartate (+ @sarahdimeloe.bsky.social @lindavsinclair.bsky.social); & reviewing the strong influence of iron on immunity @joefrost.bsky.social www.nature.com/articles/s41... www.nature.com/articles/s41...
Iron and the immune system - Nature Reviews Immunology
Iron is crucial for cellular metabolism, but its availability varies greatly within and between individuals and populations. This Review highlights how iron regulates innate and adaptive cellular and ...
nature.com
🚨Last week's #immunometabolism findings @biomednews.bsky.social 🔽🔽🔽 biomed.news/bims-imicid/... Interesting paper: Glucose-1-phosphate promotes compartmentalization of glycogen with the pentose phosphate pathway in CD8+ memory T cells
Iron deficiency causes aspartate-sensitive dysfunction in CD8+ T cells #immunometabolism www.nature.com/articles/s41...
Iron deficiency causes aspartate-sensitive dysfunction in CD8+ T cells - Nature Communications
Iron has been shown to be necessary for the activation and differentiation of CD8+ T cells. Here the authors investigate changes in CD8+ T cell metabolism in iron limiting conditions and find that asp...
nature.com
So cool to start the afternoon session with @plantophagy.bsky.social !! Here in the @autophagyuk and @UK_proteostasis meeting
What if Tregs are not a separate lineage, but part of spontaneously activated T cells? In this work, we integrate this idea with our recent findings: Adult T cell leukemia (ATL) is not 'Treg cancer', challenging the current concept of Treg as a distinct entity.🧵(1/4) academic.oup.com/discovimmuno...
Spectrum of Treg and self-reactive T cells: single cell perspectives from old friend HTLV-1
Summary. Despite extensive regulatory T cell (Treg) research, fundamental questions on in vivo dynamics remain to be answered. The current study aims to di
academic.oup.com
Review @annualreviews.bsky.social @lindavsinclair.bsky.social @cantrell-lab.bsky.social Protein Synthesis and Metabolism in T Cells www.annualreviews.org/content/jour...
Our review on Protein Synthesis and Metabolism in T cells is out @annualreviews.bsky.social @cantrell-lab.bsky.social Protein Synthesis and Metabolism in T Cells #proteomics #immunometabolism #Tcells www.annualreviews.org/content/jour...
Protein Synthesis and Metabolism in T Cells | Annual Reviews
T lymphocytes are essential for immune responses to pathogens and tumors. Their ability to rapidly clonally expand and differentiate to effector cells following infection, and then to curb effector fu...
annualreviews.org
Very excited to be just a weekend away from this UK conference on neutrophil biology that we are hosting at Newcastle. It includes international keynotes but most important the majority of speakers are early career researchers. #immunology #inflammation #neutrophils #humandisease
ICYMI by First release - now in print @science.org Identification of APCs in the gut that promote Treg differentiation and how tolerance to food antigens is influenced by helminth infection. buff.ly/3DjNQ1e By @cicacanesso.bsky.social @danmucida.bsky.social @victora.bsky.social et al
A lovely News and Views on Samantha Pretto/Max Mazzone’s “ #Elov1 and #CD8s and #tumour paper “ - check it out! www.nature.com/articles/s42...
Disrupting cholesterol homeostasis in T cells boosts antitumour responses - Nature Metabolism
A new study identifies a mechanism by which Elovl1 deletion disrupts the canonical feedback inhibition of cholesterol synthesis in T cells. By fundamentally rewiring cholesterol metabolism, Elovl1-dep...
nature.com
📢 A new episode of the podcast is out! We chat with Dr. Luke O’Neill (@lukeanthonyoneill.bsky.social) at Trinity College Dublin (@tcddublin.bsky.social) about the metabolic regulation of immune cell function and the innate #ImmuneSystem. 🎧 Listen now: bit.ly/3QybMBq
Two weeks left to apply for a postdoc position with the Slack group! Please spread the word!
Emma Slack@mucosalimmunology.bsky.social · 2y agoInterested in microbial #metabolism, #mucosalbiology and #UTI? Then check out the open postdoctoral position in the Mucosal Immunology Group at ETH Zurich! NB: All applications must go via the ETH job portal: www.jobs.ethz.ch/job/view/JOP... Closing date: 24th Feburary 🧪🧫🔬
I'm very pleased to have two lab positions open this week, to join our team looking at immune regulation in infection and co-infection. We're especially interested in tissue-based immunity, cytokine regulation and immune metabolism. Join us! Postdoc (3y) and research technician (12mo) posts open.
Very happy to share our new preprint on hallmarks of human #lung #macrophage activation by type 2 cytokines, and metabolic control of this process 🙂. #immunometabolism #ImmunoSky 🧪 @lydiabeckeriii.bsky.social @edinburgh-uni.bsky.social www.biorxiv.org/content/10.1...
Hallmarks and metabolic regulation of type 2 activated human lung macrophages
Although human lung macrophages are heterogenous and play key roles during health and disease, the mechanisms that govern their activation and function are unclear, particularly in type 2 settings. Ou...
biorxiv.org
Okey dokey here goes! First real post - please reshare if appropriate 🙏 🔔We’ve got two postdoc level positions opening in our lab! One wet lab, one computational🔔 We’re a scientifically diverse, friendly bunch working at the interface of mitochondrial biology, metabolism & cancer in Glasgow, UK.
I am overjoyed to present our latest paper on Autophagy in CD8 Tcells! www.nature.com/articles/s41... It was, as all ‘sciencing’ is, a huge team effort. I will try to do it some justice in the following thread 😊 1/n @cantrell-lab.bsky.social @immpres.bsky.social @ajmhowden.bsky.social
Autophagy repression by antigen and cytokines shapes mitochondrial, migration and effector machinery in CD8 T cells - Nature Immunology
Sinclair et al. combine quantitative proteomics and an autophagy flux reporter to map autophagy substrates and triggers during CD8 T cell differentiation. Proteins degraded and fueled by autophagy in ...
nature.com
📢 Still time to be part of SIGNET 2025! We're accepting late-breaking abstracts until Monday. Share your latest discoveries with the immunology community in Scotland on Thursday 27 March 🔬 Don't miss this final chance! Submit here: bit.ly/4euIWLO
Fine-tuning tumor immunogenicity with mitochondrial complex I Our views on a very cool paper from @ppuigserverb.bsky.social we hope you like it! www.nature.com/articles/s43...
nature.com
Delighted to have had the opportunity to write this spotlight with @juanquintana.bsky.social and the lab, on the recent @lydialynch.bsky.social paper, showing rhythmicity of IL-17 expression in adipose! Fascinating paper, which raises loads of cool questions #immunosky www.cell.com/immunity/ful...
Interleukin-17 and fat: Timing is everything
Interleukin-17 plays a major role in controlling adipose tissue homeostasis. In a recent study published in Nature, Douglas et al. demonstrate that time-of-day-dependent expression of interleukin-17 b...
cell.com
Our Communication and Engagement Grant is open to all BSI members who want to take part in #PublicEngagement 🌟 Share your passion, build skills, and make a difference to the world of #immunology 💪 Application deadline: 1 Feb 2025 🗓️ Apply now 👉 bit.ly/3Y9j8gm
Elegant work by Jason Cyster's group "Our study demonstrates that the canonical chemoattractant receptor-ligand systems well-established for lymphocyte homeostatic trafficking to LNs are no longer the major contributors in the inflamed setting." #ImmunoSky
Inflammation switches the chemoattractant requirements for naive lymphocyte entry into lymph nodes
Lymph node swelling is a well-known symptom of infection. A shift in the chemoattractant code controls lymphocyte recruitment from blood into inflamed lymph nodes, allowing lymphocytes to accumulate so that even rare antigen-specific cells can encounter their activating antigen.
cell.com
Thank you Drs. Dan Klionsky and Dimitra Dialynaki for your commentary on our work: www.tandfonline.com/doi/epdf/10....
Identification of the mammalian VPS4A as a selective lipophagy receptor
tandfonline.com
Michael J Fox has had tremendous influence on people with Parkinson's Disease (PD), their carers, families and those working on PD. He is a shining example of what a single person is capable of. Well deserved recognition.
WuTangIsForTheChildren@wutangforchildren.bsky.social · 2y agoMichael J. Fox receiving the Presidential Medal of Freedom from Joe Biden….why is it so dusty in here now….I’m not crying, you’re crying 🥹🙏
Organelles meets dinos! 🦖🔬 Featuring Mitosaur, ERsaur, Golgisaur, Lysosaur, and others, it's such a creative take on the cellular world we know and love. 💚 Which dino-organelle is your favorite? Let us know below! 👇
LPS stimulates dynamic changes in B cell metabolism to promote proliferation https://www.biorxiv.org/content/10.1101/2024.12.16.628649v1
A proteomic map of B cell activation and its shaping by mTORC1, MYC and iron https://www.biorxiv.org/content/10.1101/2024.12.19.629506v1
