So excited to see our latest work out!

Science Magazine@science.org · 2w ago

In a new Science study, researchers report the design of synthetic RNA-guided nucleases that match or exceed the activity of natural enzymes, revealing how structure-guided design can generate functional genome-editing proteins with substantially different sequences. https://scim.ag/45fbSFf

Our AI-guided design of RNA-guided nucleases is now out in @science.org 💻🧬 We used structure and evolutionary data to generate minimal, active and divergent gene editors! www.science.org/doi/10.1126/... Congrats to @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social & team!

Structure and evolution-guided design of minimal RNA-guided nucleases

The design of RNA-guided nucleases with properties not limited by evolution can expand programmable genome-editing capabilities. However, generating diverse multidomain proteins with robust enzymatic ...

science.org

🧪 Double pub day! I got to catch up with Nobel Prize-winner Jennifer Doudna about her new study crafting synthetic CRISPR proteins that perform gene editing more efficiently than their naturally-occurring counterparts! www.nature.com/articles/d41...

CRISPR gets a power boost from AI-designed ‘molecular scissors’

Researchers used artificial intelligence to design functional CRISPR enzymes not seen in nature.

nature.com

Thanks to @nature.com and @scattercushion.bsky.social for featuring our latest work published in @science.org!

Nature@nature.com · 3w ago

Scientists have harnessed AI models to create synthetic CRISPR proteins that edit the genome more efficiently than those seen in nature go.nature.com/4f8AN3n

So excited to see our paper out in Science! Beyond grateful to my co-leads @petrskopintsev.bsky.social @evandeturk.bsky.social, all co-authors & collaborators, everyone in Doudna lab, and @innovativegenomics.bsky.social Being EsAIn in the DouDNA lab, it couldn't have been any other way ✨

Doudna Lab@doudna-lab.bsky.social · 3w ago

Our AI-guided design of RNA-guided nucleases is now out in @science.org 💻🧬 We used structure and evolutionary data to generate minimal, active and divergent gene editors! www.science.org/doi/10.1126/... Congrats to @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social & team!

It's out! Check out the D-LabSky thread below 👇 Pleasure working with and learning from @petrskopintsev.bsky.social @isabelesain.bsky.social and the whole team on this oen

Doudna Lab@doudna-lab.bsky.social · 3w ago

Our AI-guided design of RNA-guided nucleases is now out in @science.org 💻🧬 We used structure and evolutionary data to generate minimal, active and divergent gene editors! www.science.org/doi/10.1126/... Congrats to @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social & team!

Proud to present our work, published today in @science.org! My deepest gratitude to @isabelesain.bsky.social, @evandeturk.bsky.social, Jennifer and our collaborators in the Doudna, Cate, Banfield, and Jacobsen labs, and the @innovativegenomics.bsky.social! I hope you enjoy reading it! ✨

Doudna Lab@doudna-lab.bsky.social · 3w ago

Our AI-guided design of RNA-guided nucleases is now out in @science.org 💻🧬 We used structure and evolutionary data to generate minimal, active and divergent gene editors! www.science.org/doi/10.1126/... Congrats to @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social & team!

New pre-print 📣 “Undruggable” cancer mutations remain very hard to target with current modalities. What if we could instead sense mutant transcripts and convert that recognition into selective cell killing? Check out this work led by postdoc Jingkun Zeng: www.biorxiv.org/content/10.6...

Synthetic microbiomics 🙌 Genome-scale editing with bridge recombinases - Congrats to @jayman1466.bsky.social @sophswartz.bsky.social, Agnes & team!

Cress Lab@cresslab.bsky.social · 3mo ago

Our lab is proud to present our latest work harnessing Bridge Recombinase for genome-scale editing in diverse bacteria, microbiome editing, and programmable horizontal gene transfer.

An RNA-guided system that recognizes a noncontiguous DNA code? 🤯 Check out the latest work from the Doudna Lab on the discovery of VIPR. Really exciting biology!

Doudna Lab@doudna-lab.bsky.social · 3mo ago

We present VIPR, a phage-encoded RNA-guided system that recognizes DNA with a noncontiguous code unlike CRISPR. Tiny, programmable, possibly ancestral to CRISPR immunity; VIPR is built around a striking RNA-DNA-DNA triplex.

How do the ancestors of CRISPR-Cas unwind DNA and how can this lead to better genome editing? With our collaboration between @doudna-lab.bsky.social x @jacobsenucla.bsky.social x Zev Bryant's lab x @savagecatsonly.bsky.social we've uncovered the secrets behind TnpB's dynamics!

New preprint 👉Doudna x Bryant x Jacobsen x Savage collaboration! Work led by @zehanzhou.bsky.social, I. Saffarian-Deemyad, @honglue.bsky.social, T. Weiss We dissect how stepwise DNA unwinding gates TnpB genome editing, revealing how unwound DNA states enhance cleavage www.biorxiv.org/content/10.6...

Stepwise DNA unwinding gates TnpB genome-editing activity

TnpB is a compact RNA-guided endonuclease and evolutionary ancestor of CRISPR-Cas12 that offers a promising platform for genome engineering. However, the genome-editing activity of TnpBs remains limit...

biorxiv.org

Excited to share our first work on protein design! Huge thanks to the entire team, and especially to Bel, Evan, to the Doudna, Jacobsen, Cate, Banfield labs, all co-authors, and my D-lab mates! 💫

Doudna Lab@doudna-lab.bsky.social · 8mo ago

✨New preprint! 🧵1/4 Excited to share our work on AI-guided design of minimal RNA-guided nucleases. Amazing work by @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social et al! Multi-lab collaboration @banfieldlab.bsky.social @jhdcate.bsky.social @jacobsenucla.bsky.social🧬 🔗👇

Excited to share our work designing genome editors with machine learning! 🧬💻 Huge thanks to @petrskopintsev.bsky.social @isabelesain.bsky.social @doudna-lab.bsky.social and all co-authors. Check out the thread for a summary of what we found 👇

Doudna Lab@doudna-lab.bsky.social · 8mo ago

✨New preprint! 🧵1/4 Excited to share our work on AI-guided design of minimal RNA-guided nucleases. Amazing work by @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social et al! Multi-lab collaboration @banfieldlab.bsky.social @jhdcate.bsky.social @jacobsenucla.bsky.social🧬 🔗👇

Big congrats to @petrskopintsev.bsky.social and @isabelesain.bsky.social !

Doudna Lab@doudna-lab.bsky.social · 8mo ago

✨New preprint! 🧵1/4 Excited to share our work on AI-guided design of minimal RNA-guided nucleases. Amazing work by @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social et al! Multi-lab collaboration @banfieldlab.bsky.social @jhdcate.bsky.social @jacobsenucla.bsky.social🧬 🔗👇

First co-led work from the Doudna lab 🤜🤛 We designed minimal RNA-guided nucleases with AI, and even did Cryo-EM during an earthquake (!) Huge honor working with @petrskopintsev.bsky.social, @evandeturk.bsky.social, Jennifer Doudna and co-authors, everyone at D-Lab and @innovativegenomics.bsky.social

Doudna Lab@doudna-lab.bsky.social · 8mo ago

✨New preprint! 🧵1/4 Excited to share our work on AI-guided design of minimal RNA-guided nucleases. Amazing work by @petrskopintsev.bsky.social @isabelesain.bsky.social @evandeturk.bsky.social et al! Multi-lab collaboration @banfieldlab.bsky.social @jhdcate.bsky.social @jacobsenucla.bsky.social🧬 🔗👇

Incredible work by @owentuck.bsky.social, Jason Hu et al 👏🏼 Antiviral systems repeatedly acquire nuclease–protease pairs to fight viruses ⚔️

Innovative Genomics Institute@innovativegenomics.bsky.social · 9mo ago

New in Science Magazine from the Doudna Lab and first author Owen Tuck: Recurrent acquisition of nuclease-protease pairs in antiviral immunity. Read here: https://ow.ly/pIWQ50XtEtM

Super cool work by @erinedoherty.bsky.social and @jnoms.bsky.social ! 💪🏼 Viruses hack bacterial immunity by breaking down the nucleotide signals that trigger defence 🦠🧬

Erin Doherty@erinedoherty.bsky.social · 8mo ago

Work I co-led with @jnoms.bsky.social is now online at @cp-cellhostmicrobe.bsky.social ! We revealed a previously unrealized diversity of viral immune-evasion proteins that selectively destroy different cyclic nucleotide signals used in bacterial immunity. www.sciencedirect.com/science/arti...

👩🏻‍🔬La investigadora española @isabelesain.bsky.social ha sido galardonada por su tesis doctoral contra el #cáncer en el @cruk-mi.bsky.social. 🗣️"El genoma humano es como un libro que tiene muchos capítulos". 🎬 En @aragontv.bsky.social. www.cartv.es/aragonnotici...

So honored to receive the Best Thesis Prize❤️ @cambridgeuni.bsky.social @cruk-ci.bsky.social still feels surreal. Thank you to CRUK, my supervisors for believing in my ideas, and my former lab members, friends and family for always supporting me. So grateful to be part of such an inspiring community!

Cancer Research UK Cambridge Institute@cruk-ci.bsky.social · 2y ago

Congratulations to @isabelesain.bsky.social, who is awarded the 2024 CRUK Cambridge Institute PhD Thesis Prize. In her doctoral research, Bel discovered a new molecular mechanism in cancer gene regulation opening up a new therapeutic window for cancer treatments. www.cruk.cam.ac.uk/news/bel-esa...

🚨 The Alker Lab is starting at the University of Rhode Island in early 2025 and we will be hiring! 🚨 If you are interested in marine microbiology, bacterial genetics and/or symbiosis, please consider reaching out! Repost to help spread the word 🪸🧪🧬🌊