That's a frigging peptide spectrum match.. Playing with endogenous peptides on mass spec is funny.. 😊😊

Hello #Astral users, Have you ever come across the ion foil adjustment failure error?? We have started seeing it since the tune was upgraded to 2.2 recently during the PM. The error interferes with the system getting ready to run a sample in the step where astral mass accuracy is checked with ICS.

One of the most overlooked factors in maintaining consistent, high-quality spray in nano-scale liquid chromatography is something you handle every time you connect your column: your capillary fitting. Thankfully, a little care & a few simple habits go a long way. Here's what to know: bit.ly/4zbjHd4

Caring for your capillary fitting: small habits for stable spray

One of the most overlooked factors in maintaining consistent, high-quality spray in nano-scale liquid chromatography is something you handle every time you connect your column: your capillary fitting ...

bit.ly

A question to mass spectrometrists in the core facilities around the UK and Europe.. How are you finding the cost of S-traps for proteomics applications?? It looks like incorporation of S-traps in the workflow adds an additional £8 per sample.. It feels extraordinarily expensive..

Our article "Common xenobiotics modulate gut microbial responses to low‑calorie sweeteners in vitro" is out! link.springer.com/article/10.1... With @kiranrpatil.bsky.social, @indraroux.bsky.social, @rbradley.bsky.social, @skamrad.bsky.social, @simonemozzachiodi.bsky.social, @itsbinir.bsky.social.

Common xenobiotics modulate gut microbial responses to low‑calorie sweeteners in vitro - Molecular Systems Biology

The gut microbiota is implicated in adverse effects associated with low-calorie sweeteners. Yet, the direct impact of sweeteners on gut bacteria remains largely uncharacterized. Here, we report intera...

link.springer.com

Hey, #TeamMassSpec! Interested in a career in academia? #ASMS will host 2 free Zoom panel discussions ft. college faculty sharing insights on preparing your academic job application package and what to expect during the interview process. Register now! https://bit.ly/4c1VrR5

Never been a fan of the so-called number game.. However, it is indeed exciting when you get to see 100 precursors identified per second from biological samples, not just from a std digest.. Pretty stoked by having a front raw seat to witness the technology evolution through ages..😊😊 #MassSpecMusings

The EMBL #ARISE2 Fellowship Programme, funded by MSCA, opens for applications 30 June. This unique opportunity trains researchers to lead #ResearchInfrastructures & innovate in #LifeSciences. 🧪 🗓️ Join our webinar on Thursday, 2 July at 09:30 CET: embl-org.zoom.us/webinar/regi... #careers

#TeamMassSpec, may I please check if there is a lab where Thermo #Ardia is seamlessly connected to multiple mass specs and process proteomics data processing and management on the server itself?? If yes, can I please have a brief chat?? I am interested in hearing more about it.. Thanks very much..

What is the magic soup in the PreOmics iST kit, I wonder.. It is getting me 6000 proteins from tiny FFPE samples!! Whereas the conventional workflow is hardly touching 1000 proteins!!

Another UK chemistry department in trouble - this time Sheffield. savesheffieldchemistry.netlify.app

Is there any option to remove the contaminant proteins prior to normalisation in #Spectronaut?? Essentially, I would like to identify them, but don't want to include their abundance values for cross run normalisation.. is there a possibility?? Thanks very much..

Hello #Spectronaut users and manufacturers.. Is there any option to export both the raw and normalized PG.Quantity values?? Is it not possible at all or am I missing something here?? Thanks very much..

A nature-inspired ion trap for parallel manipulation of ions on a massive scale www.science.org/doi/... --- #proteomics #prot-paper

Wow! www.nature.com/articles/s41... Here we report all five canonical nucleobases—purines (adenine and guanine) and pyrimidines (cytosine, thymine and uracil)—in samples returned from the C-type asteroid (162173) Ryugu

A complete set of canonical nucleobases in the carbonaceous asteroid (162173) Ryugu - Nature Astronomy

Samples returned from the asteroid Ryugu contain all five canonical nucleobases (A, G, C, T and U). Their presence in Ryugu and Bennu supports the hypothesis that carbonaceous asteroids contributed to...

nature.com

Setting up a decent DDA method on Astral is so mentally taxing.. It isn't meant to follow any of the data dependency criteria.. Enforcing it to follow the criteria is where it gets taxing.. Wonder how it is working for those MS2-TMT workflows.. 🤔🤔

It is an incredible honour to be featured in the Faces of Mass Spectrometry series of JASMS and share the story of a nerdy mass spectrometrist.. who despite being an introvert, always love to talk about how wonderful mass spectrometry is.. ☺️☺️ Proud to be a part of #TeamMassSpec and #FeMS.. Thanks..

Faces of Mass Spectrometry/Bini Ramachandran

pubs.acs.org

Today at 13:50, on the dot, the sequences running on both Ascend and Eclipse came to an abrupt stop.. Just at random.. In the middle of a run.. Neo kept running but no MS acquisition.. Had to restart Xcalibur and reconnect LC to resume the sequence.. Very spooky!!! #MassSpecMusings

Anybody doing proteomics on FACS sorted cells? People are using BSA coated tubes to avoid cell adhesion onto the walls. BSA is all over the protein extract. How to avoid this BSA? Saw Polyvinyl alcohol (PVA) can be used in place of BSA. Any experience with its compatibility for downstream MS? Thanks

"This work provides insight to help future proteomics study designers make more informed decisions on whether physical normalization is necessary for their experiments" Guess I am that old rigid mass spectrometrist now who finds it hard to keep an open mind to new concepts.. 😉😉

PastelBio@pastelbio.bsky.social · 7mo ago

Is Protein Quantification and Physical Normalization Always Necessary in Proteomics? www.biorxiv.org/cont... --- #proteomics #prot-preprint

I have all these glycan specific fragments from a glycopeptide analysis.. Any software suggestions for identifying both the peptide and the attached glycan composition from such spectra?? Thanks very much.. #glycotime #TeamMassSpec

#TeamMassSpec Question for XL-MS practitioners.. Looking for a suitable crosslinker to stabilise a protein complex in vitro.. What are the pros and cons for MS-cleavable vs non-cleavable cross-linkers?? Is PhoX a good choice?? Any recommendations for MS-cleavable cross-linkers?? Thanks very much..

The Spatial Omics Summer School offers a structured, hands-on introduction to spatially resolved targeted transcriptomics, proteomics, and metabolomics. If you want to join, submit your application by 2 February 2026. https://www.vibconferences.be/events/spatial-omics-summer-school

(J Proteom) Two-dimensional electrophoresis-based proteomics reveals soybean seed hypocotyl proteoforms: Publication date: Available online 17 January 2026 Source: Journal of Proteomics Author(s): Jian-Zhong Tan, Hiroyuki Kagawa, Keiko Kizawa, Hisashi Hirano #MassSpecRSS

Two-dimensional electrophoresis-based proteomics reveals soybean seed hypocotyl proteoforms

Publication date: Available online 17 January 2026 Source: Journal of Proteomics Author(s): Jian-Zhong Tan, Hiroyuki Kagawa, Keiko Kizawa, Hisashi Hirano

dlvr.it

Big evaluation of our Orbitrap Astral Zoom mass spectrometer by the Olsen group, now peer reviewed, published and open access. www.sciencedirect.com/science/arti...

Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer

High-throughput proteomics is critical for understanding biological processes, enabling large-scale studies such as biomarker discovery and systems bi…

sciencedirect.com

PastelBio@pastelbio.bsky.social · 8mo ago

Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer www.sciencedirect.co... --- #proteomics #prot-paper

(JASMS) [ASAP] Faces of Mass Spectrometry/Khatereh Motamedchaboki: Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00430 (RSS) #MassSpecRSS #JASMS

[ASAP] Faces of Mass Spectrometry/Khatereh Motamedchaboki

Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00430

dlvr.it

Vanquish Duo as Neo Mono with EasySpray column setup.. Works quite alright.. Guess have to get uPAC to test the dual column setup at nano flow rate..

Bini Ramachandran @itsbinir.bsky.social · 9mo ago

#TeamMassSpec Can someone please help me remember whether the Vanquish Duo launched at ASMS2024 was meant for nanoLC applications?? Somehow, I remember it as the potential high speed solution from Thermo for Astral high SPD applications.. However, the Duo I got hold of here is a microflow version!!

A slide from a presentation I gave in 2013 on mass spectrometry proteomics.. Ironically, still a relevant topic to include this slide as such in a talk I am preparing to give after the new year..

Holidays started.. And the festive decor is on.. 🎉🎉#MassSpecHolidayDecor

Post-doc positions: "Academic freedom is under pressure today. This requires rescue havens of free research. ... [we] invite early career researchers, whose work is restricted due to political pressure in the USA..." uni-freiburg.de/frias/call-f...