Make sure to check our latest collaboration with @kaitezhang.bsky.social and @vram142.bsky.social. An effort to move towards simulating non-ideal, "in vivo" chromatin, and learning lots about chromatin organisation while doing it! @juliamaristany.bsky.social @rcollepardo.bsky.social

Vijay Ramani@vram142.bsky.social · 2mo ago

JIT 4 summer, the latest preprint from our lab, brilliantly led by @kaitezhang.bsky.social w/ support from Collepardo lab (@juliamaristany.bsky.social / @janhuemar.bsky.social). www.biorxiv.org/content/10.6... describes a new single-molecule epigenomic method, & discoveries it enables ... (1/n)

My first, first author paper is now on #ScienceAdvances ! Linker histone H1 is a liquid-like "glue" condensing chromatin, which revises textbooks! 📖✨ science.org/doi/10.1126/sc… Huge thanks to @kazu-maeshima.bsky.social , for supervision. Amazing collab with @rcollepardo.bsky.social ’s group! 1/

Our work on the linker histone is now out in Science Advances! We show that H1 behaves as a liquid-like glue that helps organize chromatin in living cells. Very happy to have worked together with @kazu-maeshima.bsky.social and @masaashimazoe.bsky.social

Kazuhiro Maeshima@kazu-maeshima.bsky.social · 4mo ago

Our H1 paper is out #ScienceAdvances: www.science.org/doi/10.1126/... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

New paper alert from the group!! 🚨: DNA flexibility tips the balance between stability and plasticity in nucleosomes One of the works from my PhD, co-led alongside @nachper.bsky.social, is finally out! Work from @rcollepardo.bsky.social & @janhuemar.bsky.social ⬇️ www.biorxiv.org/content/10.6... ⬆️

How do DNA sequence and histone chemistry tune nucleosome stability and plasticity? Check out our latest work to find out. Now available on bioRxiv! www.biorxiv.org/content/10.6... @juliamaristany.bsky.social @janhuemar.bsky.social @rcollepardo.bsky.social Share with your colleagues!! ⬇️⬇️⬇️

Julia Maristany@juliamaristany.bsky.social · 6mo ago

New paper alert from the group!! 🚨: DNA flexibility tips the balance between stability and plasticity in nucleosomes One of the works from my PhD, co-led alongside @nachper.bsky.social, is finally out! Work from @rcollepardo.bsky.social & @janhuemar.bsky.social ⬇️ www.biorxiv.org/content/10.6... ⬆️

A Goldilocks zone of DNA flexibility defines stable yet plastic nucleosomes, tuned by histone chemistry https://www.biorxiv.org/content/10.64898/2026.02.16.706184v1

New preprint alert! Very proud of this work, lots of hard work that turned into a really cool story! This was my first chance to step into a senior role, but most importantly, it means far more to me than just the science. You should always say yes to new projects. www.biorxiv.org/content/10.6...

Julia Maristany@juliamaristany.bsky.social · 6mo ago

Please visit the preprint! We are very proud of this work - but also, this paper means a lot to me: Jan and I met and started dating while working on it. Later, we worked on many more projects together, but this was the first. Some projects shape your science - this one shaped my life, too ❤️

Congratulations to @janhuemar.bsky.social (JRF at Catz since 2023) who is part of an international team of scientists responsible for a long-awaited visualisation of liquid droplets forming inside a cell known as condensates, key for processes such as DNA repair: caths.cam.ac.uk/cond... @cam.ac.uk

Visualising the creation of biomolecular condensates

A Junior Research Fellow at St Catharine's is part of an international team of scientists responsible for a long-awaited visualisation of liquid droplets forming inside a cell nucleus known as condensates, which help regulate essential processes such as gene expression, DNA repair and stress responses.

caths.cam.ac.uk

Super excited to share that our paper is now out in @science.org. Lots of work, but also lots of fun getting this out. Be sure to check it out! www.science.org/doi/10.1126/... Also, follow: @huabin-zhou.bsky.social @juliamaristany.bsky.social @kieran-russell.bsky.social @rcollepardo.bsky.social

Multiscale structure of chromatin condensates explains phase separation and material properties

The structure and interaction networks of molecules within biomolecular condensates are poorly understood. Using cryo–electron tomography and molecular dynamics simulations, we elucidated the structur...

science.org

Jan Huertas@janhuemar.bsky.social · 2y ago

[2/6] How’d we do it? High-resolution cryo-ET captured chromatin condensates at nanometer detail, while simulations brought the histone tails and nucleosome dynamics to life. Together, these tools showed how molecular interactions drive mesoscale chromatin behavior.

Very excited to present OpenCGChromatin🔥🔥🔥 A new coarse-grained model that probes full chromatin condensates at near-atomistic resolution to reveal the molecular regulation of chromatin structure and phase separation Brilliantly led by @kieran-russell.bsky.social, with the Rosen and Orozco groups

bioRxiv Biophysics@biorxiv-biophys.bsky.social · 9mo ago

Near-atomistic simulations reveal the molecular principles that control chromatin structure and phase separation https://www.biorxiv.org/content/10.1101/2025.11.17.688899v1

So exciting to see this amazing paper out! Many thanks to @jojdavies.bsky.social for inviting us to contribute with our models to this fascinating story! @rcollepardo.bsky.social @juliamaristany.bsky.social

James Davies@jojdavies.bsky.social · 10mo ago

Our latest paper has just been published in Cell! doi.org/10.1016/j.ce... We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.

🚨 🚨 🚨 New preprint alert!!! 🚨 🚨 🚨 In the past, we have learnt that Oct4 can induce nucleosome breathing on the mono-nucleosome level. But what happens when you have a fibre of multiple nucleosomes? www.biorxiv.org/content/10.1... @rcollepardo.bsky.social @juliamaristany.bsky.social

1/ New preprint alert! In collaboration between the Rosen, Redding, Collepardo-Guevara & Gerlich labs, we uncover a surprising principle of chromosome organisation: electrostatic repulsion positions centromeres at the chromosome surface during mitosis. 🔗 doi.org/10.1101/2025...

An electrostatic repulsion model of centromere organisation

During cell division, chromosomes reorganise into compact bodies in which centromeres localise precisely at the chromatin surface to enable kinetochore-microtubule interactions essential for genome se...

doi.org

🚨 New paper out in Nature Comms! From the Rosen, Redding and Collepardo Labs, we uncover how #nucleosome spacing fine-tunes the architecture of #chromatin condensates. 👇 A short thread on how physical #genome organization emerges from molecular interactions: www.nature.com/articles/s41...

Nucleosome spacing can fine-tune higher-order chromatin assembly - Nature Communications

Internucleosomal linker length alters the stability and dynamics of chromatin condensates by shifting the balance between inter- and intramolecular interactions. Further, by changing the linker length...

nature.com

So excited to finally see our CTPR-condensate work published! Well done Chris, Mateo, Julia et al. ❤️ @chemicalscience.rsc.org @phar.cam.ac.uk @rcollepardo.bsky.social @juliamaristany.bsky.social doi.org/10.1039/D5SC...

🌱Join us for the first #FragileNucleosome seminar of this spring! We are excited to host @juliamaristany.bsky.social & @chribue.bsky.social this week! If you have registered before you can join from the same link, if not, don't forget to register! us06web.zoom.us/webinar/regi...

Excited to share our newest collaboration with @kazu-maeshima.bsky.social and @masaashimazoe.bsky.social, where we show that H1 acts as a liquid-like glue in chromatin. Go check the paper on the biorXiv!

Kazuhiro Maeshima@kazu-maeshima.bsky.social · last yr.

Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

We are happy to share our work on local #nucleosome dynamics integrating #temperature inputs into an #epigenetic switching mechanism. ⚠️ Preprint alert!! www.biorxiv.org/content/10.1...

Cold-induced nucleosome dynamics linked to silencing of Arabidopsis FLC

Temperature influences nucleosome dynamics, and thus chromatin, to regulate gene expression. Such mechanisms underlie the epigenetic silencing of Arabidopsis FLOWERING LOCUS C (FLC) by prolonged cold....

biorxiv.org

Proud to have the final version of our paper on mutations + condensates + scaling laws published in @elife.bsky.social! Work led by @juliamaristany.bsky.social w/ @rcollepardo.bsky.social ! Read the digest here: elifesciences.org/digests/9906...

Disrupting droplets

Mutations in certain protein regions have predictable effects on the stability of cellular droplets and the risk of harmful protein clumps forming inside them.

elifesciences.org

Are you in #BPS2025? Come to join us at the MGO Symposium this afternoon!

Rosana Collepardo@rcollepardo.bsky.social · 2y ago

Who's going to #BPS2025? Please join us at the MGO Symposium @mgo-bps.bsky.social on Saturday 1:30 pm Room 511ABC Featuring experiments, simulations, and all the scales by @serenasanulli.bsky.social @melikel.bsky.social @maristizabal.bsky.social @janhuemar.bsky.social and more Please repost! 🚀✨

Who's going to #BPS2025? Please join us at the MGO Symposium @mgo-bps.bsky.social on Saturday 1:30 pm Room 511ABC Featuring experiments, simulations, and all the scales by @serenasanulli.bsky.social @melikel.bsky.social @maristizabal.bsky.social @janhuemar.bsky.social and more Please repost! 🚀✨

Exciting new preprint alert! 🚨 The Collepardo lab, teaming up with Huabin Zhou and the Rosen lab we took a deep, high-resolution look into chromatin condensates! [1/6] #Chromatin #MD @rcollepardo.bsky.social @juliamaristany.bsky.social www.biorxiv.org/content/10.1...

The Multiscale Genome Organisation is now on Bluesky! Our first post is to announce our next webinar. Join us next week on Tuesday, Jan. 28th at 12:00 E.T. for two exciting talks by Pablo Dans and @gfudenberg.bsky.social See you all there! Link: mit.zoom.us/j/91715298970

Happy holidays from the Collepardo group ♥️

Excited to be a part of this story!!! Check out our new manuscript exploring how linker DNA length controls chromatin's ability to phase separate

Rosana Collepardo@rcollepardo.bsky.social · 2y ago

Is chromatin ordered or disordered? It all depends on the linker DNA length. Check our latest work with Mike Rosen and Sy Redding. We explore how changes in linker DNA length (as small as 1 bp) fine-tune chromatin structure, between order and disorder, and the properties of chromatin droplets

Is chromatin ordered or disordered? It all depends on the linker DNA length. Check our latest work with Mike Rosen and Sy Redding. We explore how changes in linker DNA length (as small as 1 bp) fine-tune chromatin structure, between order and disorder, and the properties of chromatin droplets

bioRxiv Biophysics@biorxiv-biophys.bsky.social · 2y ago

Nucleosome Spacing Can Fine-Tune Higher Order Chromatin Assembly https://www.biorxiv.org/content/10.1101/2024.12.23.627571v1