Great to be back at CSHL!!! Tomorrow I’ll be giving a talk at the CSHL meeting “Genome Organization & Nuclear Function” #cshlnucleus Can’t wait to share our work and discuss!

Check out our latest work in collaboration with @kazu-maeshima.bsky.social and @masaashimazoe.bsky.social We show that H1 is a dynamic, multivalent “liquid-like” glue of chromatin in cells: it bridges multiple nucleosomes & exchanges partners frequently to enable both compaction and flexibility

Kazuhiro Maeshima@kazu-maeshima.bsky.social · 4mo ago

Our H1 paper is out #ScienceAdvances: www.science.org/doi/10.1126/... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

I am not afraid to say it: this is the most fundamental advance in our understanding of chromatin structure since the zig-zag fiber model. Congratulations!

Kazuhiro Maeshima@kazu-maeshima.bsky.social · 4mo ago

Our linker histone H1 paper is featured on the cover of the April 10 issue of #ScienceAdvances. www.science.org/doi/10.1126/... A molecular dynamics simulation of nucleosomes and linker histone H1, with single-H1 trajectories (background). 🎉 Full cover caption: www.science.org/toc/sciadv/1...

A new model provides insight into chromatin organization and dynamics in living cells. Learn more in this week’s issue of #ScienceAdvances: https://scim.ag/4sYMeyS

Our H1 paper is out #ScienceAdvances: www.science.org/doi/10.1126/... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

My first, first author paper is now on #ScienceAdvances ! Linker histone H1 is a liquid-like "glue" condensing chromatin, which revises textbooks! 📖✨ science.org/doi/10.1126/sc… Huge thanks to @kazu-maeshima.bsky.social , for supervision. Amazing collab with @rcollepardo.bsky.social ’s group! 1/

My student Aoi Otsuka ’s PhD work is now officially published @csf-jscb.bsky.social! 🔬 Single-nucleosome imaging uncovers biphasic local chromatin dynamics in an oncogene-inducible human carcinogenesis model (1–3 d: same, 5–7 d: ↑, back by week 4). Congrats! www.jstage.jst.go.jp/article/csf/...

My PhD students Aoi Otsuka @masaashimazoe.bsky.social et al. published @csf-jscb.bsky.social 🎉 Single-nucleosome imaging in an oncogene-inducible human carcinogenesis model shows biphasic chromatin dynamics (1–3 d same; 5–7 d ↑; back by week 4). Congrats! 👉 doi.org/10.1247/csf....

Excited to share our latest fully in-house paper! 🎉 We show how cohesin integrates loop extrusion with sister tethering to guide the homology search during DNA repair. Huge congratulations to all authors, and to @fedeteloni.bsky.social for leading this work 👏 We’re excited to see his next steps!

Federico Teloni@fedeteloni.bsky.social · 8mo ago

I am happy to share that my postdoctoral work in the @gerlichlab.bsky.social at @imbavienna.bsky.social is finally out 🎉! Our study reveals how cohesin guides focused and accurate homology search. Read more 👉 www.science.org/doi/10.1126/... Follow along for key insights and updates! 🧵

I am happy to share that my postdoctoral work in the @gerlichlab.bsky.social at @imbavienna.bsky.social is finally out 🎉! Our study reveals how cohesin guides focused and accurate homology search. Read more 👉 www.science.org/doi/10.1126/... Follow along for key insights and updates! 🧵

Cohesin guides homology search during DNA repair using loops and sister chromatid linkages

Accurate repair of DNA double-strand breaks (DSBs) is essential for genome stability, and defective repair underlies diseases such as cancer. Homologous recombination uses an intact homologous sequenc...

science.org

Our Science paper is out! Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group News: bit.ly/4avnkAr and bit.ly/3XBGVHS Great perspective by @vram142.bsky.social +K Zhang

Di Jiang@dijiang319.bsky.social · 8mo ago

@science.org 🧬🔬 Multiscale structure of #chromatin condensates explains phase separation and material properties | Science www.science.org/doi/10.1126/... @janhuemar.bsky.social et al.

A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...

Check out our new work, led by talented @janhuemar.bsky.social, where we uncover that pioneer factor Oct4 remodels chromatin for DNA access not by opening it but by exploiting nucleosome breathing and forming clusters 🔥🔥🔥

Jan Huertas@janhuemar.bsky.social · 10mo ago

🚨 🚨 🚨 New preprint alert!!! 🚨 🚨 🚨 In the past, we have learnt that Oct4 can induce nucleosome breathing on the mono-nucleosome level. But what happens when you have a fibre of multiple nucleosomes? www.biorxiv.org/content/10.1... @rcollepardo.bsky.social @juliamaristany.bsky.social

🚨 🚨 🚨 New preprint alert!!! 🚨 🚨 🚨 In the past, we have learnt that Oct4 can induce nucleosome breathing on the mono-nucleosome level. But what happens when you have a fibre of multiple nucleosomes? www.biorxiv.org/content/10.1... @rcollepardo.bsky.social @juliamaristany.bsky.social

This year’s #NobelPrize in Chemistry has gone to the architects of molecular “cages” that could be used for everything from carbon capture to drug delivery. https://scim.ag/4mTE1Z6

This year’s #NobelPrize in Physiology or Medicine has been awarded to three scientists for discovering how the immune system distinguishes friend from foe. https://scim.ag/3Wt5rdi

Medicine Nobel goes to three researchers who identified immune system’s security guards

Mary Brunkow, Fred Ramsdell, and Shimon Sakaguchi discovered regulatory T cells that prevent autoimmune disease

scim.ag

@kazu_maeshima from the Genome Dynamics Laboratory at the National Institute of Genetics (@NIG_idenken) will present groundbreaking research on chromatin organization and behavior in live cells revealed by super-resolution imaging. nyulangone.zoom.us/webinar/regi... #chromatin2025

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing. www.biorxiv.org/cgi/content/...

One more movie! Using the RL algorithm (from pnas.org/doi/10.1073/...), @masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility. Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)

Shiori Iida@shiori-iida.bsky.social · 11mo ago

Our new preprint is out! We combined single-nucleosome imaging and 3D-SIM to reveal: 🔹 Euchromatin forms condensed domains, not open fibers 🔹Cohesin loss increases nucleosome mobility without decompaction 🔹Cohesin prevents neighboring domain mixing Full story & movies👇

Euchromatin is mostly condensed⁉️ With @shiori-iida.bsky.social , We revealed the detailed structure of euchromatin using super-resolution imaging. Cohesin regulates the function of euchromatin via chromatin dynamics! (1/n)

Kazuhiro Maeshima@kazu-maeshima.bsky.social · 11mo ago

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing. www.biorxiv.org/cgi/content/...

Pleased to see this study out, combining experimental work from our lab at the @cbitoulouse.bsky.social, in particular by @nathaliebastie.bsky.social and @beckof.bsky.social, and from Laura Chaptal and @alengronne.bsky.social at IGH Montpellier @cnrs.fr @cnrsbiologie.bsky.social Congrats to all!

RNA Pol II-based regulations of chromosome folding

How transcription and cohesin complexes interact to regulate 3D genome organization remains unclear. Chapard et al. dissect their respective contributions and their interplay in shaping the genome arc...

cell.com

And again, Fantastic meeting! 🔬 Single-molecule studies are advancing biology into new territory—thrilled to be involved. 💡 #EMBLSingleMolecule

I had an amazing time at EMBL Heidelberg! I was absolutely impressed by the facilities—everything operates on a completely different scale here. Definitely hope to work in an environment like this someday.

Honored to receive 1st Place Poster Award 🏆 at the EMBL meeting "Gene Regulation: One Molecule at a Time"! Thank you to all who visited and engaged in exciting discussions. Grateful to the organizers for an excellent event! #EMBLSingleMolecule

🔬 #EMBLSingleMolecule poster session highlights 📜 Big ideas, bold questions, and lots of sticky notes! From molecular mechanics to late-breaking results – the poster session was buzzing with energy and conversation 💬 Loved seeing early-career researchers shine!

Our new preprint 🧬🔗 www.biorxiv.org/content/10.1... BRD4-NUT forms liquid-like condensates that locally constrain nucleosomes via BRD4-mediated crosslinking— physical control of #chromatin by #LLPS transcription condensates. @semeigazin.bsky.social @katsuminami.bsky.social @masaashimazoe.bsky.social

Our new paper is out@ScienceAdvances👇 www.science.org/doi/10.1126/... 🧬Our Repli-Histo labeling marks nucleosomes in euchromatin and heterochromatin in live human cells. 🔍 @katsuminami.bsky.social et al. have developed a chromatin behavior atlas within the nucleus. 1/2

My first, first author paper is now on bioRxiv! 🤩 Linker histone H1 is a liquid-like "glue" condensing chromatin, which revises textbooks! 📖✨ biorxiv.org/content/10.1... Huge thanks to my PI, @kazu-maeshima.bsky.social, for supervision. Amazing collab with @rcollepardo.bsky.social’s group!" (1/n)

My first, first author paper is now on bioRxiv! 🤩 Linker histone H1 is a liquid-like "glue" condensing chromatin, which revises textbooks! 📖✨ biorxiv.org/content/10.1... Huge thanks to my PI, @kazu-maeshima.bsky.social, for supervision. Amazing collab with @rcollepardo.bsky.social’s group!" (1/n)

Excited to share our newest collaboration with @kazu-maeshima.bsky.social and @masaashimazoe.bsky.social, where we show that H1 acts as a liquid-like glue in chromatin. Go check the paper on the biorXiv!

Kazuhiro Maeshima@kazu-maeshima.bsky.social · last yr.

Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/