Rosana Collepardo
@rcollepardo.bsky.social
Professor of Computational and Molecular Biophysics at Cambridge University. We work on multiscale modeling of chromatin and biomolecular condensates. From Mexico City 🇲🇽. Mum of two (She/Her)
That’s a wrap on this year’s Chromatin Consortium in Woods Hole! 🧬 The @danielgerlich.bsky.social , @rcollepardo.bsky.social , @vram142.bsky.social, Gibson, and Rosen labs came together to exchange ideas, run experiments, and explore chromatin organisation from different angles. 1/4
Make sure to check our latest collaboration with @kaitezhang.bsky.social and @vram142.bsky.social. An effort to move towards simulating non-ideal, "in vivo" chromatin, and learning lots about chromatin organisation while doing it! @juliamaristany.bsky.social @rcollepardo.bsky.social
Vijay Ramani@vram142.bsky.social · 3mo agoJIT 4 summer, the latest preprint from our lab, brilliantly led by @kaitezhang.bsky.social w/ support from Collepardo lab (@juliamaristany.bsky.social / @janhuemar.bsky.social). www.biorxiv.org/content/10.6... describes a new single-molecule epigenomic method, & discoveries it enables ... (1/n)
JIT 4 summer, the latest preprint from our lab, brilliantly led by @kaitezhang.bsky.social w/ support from Collepardo lab (@juliamaristany.bsky.social / @janhuemar.bsky.social). www.biorxiv.org/content/10.6... describes a new single-molecule epigenomic method, & discoveries it enables ... (1/n)
Single-molecule nucleosome spacing coordinates chromatin fiber interactions
Nucleosome spacing influences higher-order chromatin fiber organization in vitro but how this relates to cellular chromosome structure remains contentious. To address this, we developed Ligation Analy...
biorxiv.org
Check out our latest work in collaboration with @kazu-maeshima.bsky.social and @masaashimazoe.bsky.social We show that H1 is a dynamic, multivalent “liquid-like” glue of chromatin in cells: it bridges multiple nucleosomes & exchanges partners frequently to enable both compaction and flexibility
Kazuhiro Maeshima@kazu-maeshima.bsky.social · 6mo agoOur H1 paper is out #ScienceAdvances: www.science.org/doi/10.1126/... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/
How do DNA sequence and histone composition modulate nucleosome plasticity? We investigated this by comparing the behaviour of 40 chemically different nucleosomes. Check our preprint below.
Julia Maristany@juliamaristany.bsky.social · 7mo agoNew paper alert from the group!! 🚨: DNA flexibility tips the balance between stability and plasticity in nucleosomes One of the works from my PhD, co-led alongside @nachper.bsky.social, is finally out! Work from @rcollepardo.bsky.social & @janhuemar.bsky.social ⬇️ www.biorxiv.org/content/10.6... ⬆️
1/New preprint just dropped! 🔥🔥 We investigate how the genome is destroyed in apoptotic cells in a way that prevents DNA fragments from spreading beyond the dying cell 🧬⚰ Done here at @imbavienna.bsky.social & in the super @rcollepardo.bsky.social & Rosen labs: www.biorxiv.org/content/10.6...
biorxiv.org
Cryogenic electron tomography of condensed chromatin enables multiscale analysis of its structure. Learn more in a new #SciencePerspective: https://scim.ag/3Yfn1T3
A vision of chromosome organization
Cryogenic electron tomography of condensed chromatin enables multiscale analysis of its structure
scim.ag
Want to learn about how we use computational approaches at different scales to study biomolecular condensates? Check out our latest review, out in Advances in Physics X @juliamaristany.bsky.social @alinaemelianova.bsky.social @rcollepardo.bsky.social www.tandfonline.com/doi/epdf/10....
Our Science paper is out! Huge congratulations to @huabin-zhou.bsky.social, Mike Rosen, and the brilliant @janhuemar.bsky.social @juliamaristany.bsky.social and @kieran-russell.bsky.social from our group News: bit.ly/4avnkAr and bit.ly/3XBGVHS Great perspective by @vram142.bsky.social +K Zhang
Di Jiang@dijiang319.bsky.social · 10mo ago@science.org 🧬🔬 Multiscale structure of #chromatin condensates explains phase separation and material properties | Science www.science.org/doi/10.1126/... @janhuemar.bsky.social et al.
Very excited to present OpenCGChromatin🔥🔥🔥 A new coarse-grained model that probes full chromatin condensates at near-atomistic resolution to reveal the molecular regulation of chromatin structure and phase separation Brilliantly led by @kieran-russell.bsky.social, with the Rosen and Orozco groups
bioRxiv Biophysics@biorxiv-biophys.bsky.social · 10mo agoNear-atomistic simulations reveal the molecular principles that control chromatin structure and phase separation https://www.biorxiv.org/content/10.1101/2025.11.17.688899v1
Many thanks to @jojdavies.bsky.social for inviting us to take part in this exciting study, combining MCC ultra with simulations to explore how the biophysical properties of chromatin govern its structure in living cells. Simulations by @janhuemar.bsky.social and @juliamaristany.bsky.social
James Davies@jojdavies.bsky.social · 11mo agoOur latest paper has just been published in Cell! doi.org/10.1016/j.ce... We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
Check out our new work, led by talented @janhuemar.bsky.social, where we uncover that pioneer factor Oct4 remodels chromatin for DNA access not by opening it but by exploiting nucleosome breathing and forming clusters 🔥🔥🔥
Jan Huertas@janhuemar.bsky.social · 11mo ago🚨 🚨 🚨 New preprint alert!!! 🚨 🚨 🚨 In the past, we have learnt that Oct4 can induce nucleosome breathing on the mono-nucleosome level. But what happens when you have a fibre of multiple nucleosomes? www.biorxiv.org/content/10.1... @rcollepardo.bsky.social @juliamaristany.bsky.social
Julia Maristany@juliamaristany.bsky.social · last yr.🚨 New paper out in Nature Comms! From the Rosen, Redding and Collepardo Labs, we uncover how #nucleosome spacing fine-tunes the architecture of #chromatin condensates. 👇 A short thread on how physical #genome organization emerges from molecular interactions: www.nature.com/articles/s41...
We found that charge asymmetry along chromatin drives centromeres to the chromosome surface during cell division 🔥🔥🔥 If you want to know more, check our latest work in collaboration with the labs of Daniel Gerlich, Mike Rosen, and Sy Redding Simulations by superstar @juliamaristany.bsky.social
Gerlich Lab@gerlichlab.bsky.social · last yr.1/ New preprint alert! In collaboration between the Rosen, Redding, Collepardo-Guevara & Gerlich labs, we uncover a surprising principle of chromosome organisation: electrostatic repulsion positions centromeres at the chromosome surface during mitosis. 🔗 doi.org/10.1101/2025...
It was a huge treat for me personally, and the Chemistry Department more generally, to host you. Thank you for examining Pin Yu, and the inspiring talk and discussions ⭐️⭐️⭐️⭐️⭐️
Kresten Lindorff-Larsen@lindorfflarsen.bsky.social · last yr.Wonderful to be back in Cambridge for a PhD viva Thanks @rcollepardo.bsky.social and Aleks Reinhardt for inviting me and for letting me stay at Trinity while my old college St John’s was having their May Ball
So excited to finally see our CTPR-condensate work published! Well done Chris, Mateo, Julia et al. ❤️ @chemicalscience.rsc.org @phar.cam.ac.uk @rcollepardo.bsky.social @juliamaristany.bsky.social doi.org/10.1039/D5SC...
Tandem-repeat proteins introduce tuneable properties to engineered biomolecular condensates
The cell’s ability to rapidly partition biomolecules into biomolecular condensates is linked to a diverse range of cellular functions. Understanding how the structural attributes of biomolecular conde...
doi.org
In this wonderful collaboration with K Maeshima and M Shimazoe we show that H1 in living cells acts as a liquid-like glue not a driver of stiff zigzag fibers 🔥🔥🔥 Each H1 bridges multiple nucleosomes and exchanges nucleosomes frequently: boosting both compaction and dynamical behaviour of chromatin
Kazuhiro Maeshima@kazu-maeshima.bsky.social · 2y agoOur new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1... @masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/
We are excited to announce a new faculty position here in Cambridge, for researchers in computational and/or theoretical biology, based jointly in Genetics and Mathematics. Come and join us! Happy to answer questions about research, teaching and working here. www.jobs.cam.ac.uk/job/50414/
Assistant/Associate Professor in Computational Biology - Job Opportunities - University of Cambridge
Assistant/Associate Professor in Computational Biology in the Department of Applied Mathematics and Theoretical Physics at the University of Cambridge.
jobs.cam.ac.uk
To close, we have Jan Huertas from Cambridge University @janhuemar.bsky.social presenting our work in collaboration with the Rosen lab on the structure of chromatin inside condensates and the role of histone tails #BPS2025 @mgo-bps.bsky.social
María Aristizabal from Queen’s University in Canada tells us how combining experiments and simulations informs on why histone modifications are involved in cancer #BPS2025 @mgo-bps.bsky.social
Shoji Takada from Kyoto University in Japan describes his impressive multiscale simulations of transcription regulation #BPS2025 @mgo-bps.bsky.social
Serena Sanulli from Stanford University @serenasanulli.bsky.social discusses HP1 condensates and chromatin structure #BPS2025 @mgo-bps.bsky.social
Now is the turn of Paul Meneses from Taekjip Ha lab at Johns Hopkins and Harvard Medical School discussing single molecule measurements on native chromatin #BPS2025 @mgo-bps.bsky.social
Rob McGinty from University of North Carolina discusses structural mechanisms of nucleosome demethylation #BPS2025 @mgo-bps.bsky.social
Now we have Marcus Woodworth from University if Pennsylvania telling us about chromatin states in native chromatin visualised with expansion microscopy #BPS2025 @mgo-bps.bsky.social
First is Michael Poirier from Ohio State, discussing the pathways by which H1 loads into nucleosomes #BPS2025 @mgo-bps.bsky.social
Do you like chromatin, condensates, single-molecule imaging? Then you’ll love our #BPS2025 MGO symposium. TODAY at 1:30 pm
Rosana Collepardo@rcollepardo.bsky.social · 2y agoWho's going to #BPS2025? Please join us at the MGO Symposium @mgo-bps.bsky.social on Saturday 1:30 pm Room 511ABC Featuring experiments, simulations, and all the scales by @serenasanulli.bsky.social @melikel.bsky.social @maristizabal.bsky.social @janhuemar.bsky.social and more Please repost! 🚀✨
Proud to have the final version of our paper on mutations + condensates + scaling laws published in @elife.bsky.social! Work led by @juliamaristany.bsky.social w/ @rcollepardo.bsky.social ! Read the digest here: elifesciences.org/digests/9906...
Disrupting droplets
Mutations in certain protein regions have predictable effects on the stability of cellular droplets and the risk of harmful protein clumps forming inside them.
elifesciences.org
Are you in #BPS2025? Come to join us at the MGO Symposium this afternoon!
Rosana Collepardo@rcollepardo.bsky.social · 2y agoWho's going to #BPS2025? Please join us at the MGO Symposium @mgo-bps.bsky.social on Saturday 1:30 pm Room 511ABC Featuring experiments, simulations, and all the scales by @serenasanulli.bsky.social @melikel.bsky.social @maristizabal.bsky.social @janhuemar.bsky.social and more Please repost! 🚀✨