I got something I wanna talk about: parasite genomes! 🧬 Our lab at FMRP-USP is recruiting: 🔬 1 Direct PhD fellow 🧫 1 Postdoc fellow @agenciafapesp.bsky.social-funded to study how ubiquitination shapes genome replication & plasticity in Leishmania. Details in the comments👇

We’re pleased to share our new study, led by @jezieldamasceno.bsky.social, that used long-read, single molecule sequencing to reveal the full (?) picture of Leishmania DNA replication. Thanks, Mike Boemo and helpful reviewers @uofgsii.bsky.social www.sciencedirect.com/science/arti...

Leishmania major chromosomes are replicated from a single high-efficiency locus supplemented by thousands of lower efficiency initiation events

DNA replication initiates at genome sites termed origins. Previous origin mapping approaches in the populations of the protozoan parasite Leishmania m…

sciencedirect.com

Pleased 2 share our new article: single-molecule replication mapping in Leishmania using DNAscent. We show widespread stochastic origin firing and insights into genome variability. www.cell.com/cell-reports...

More recent work from the McCulloch lab! Check out Jeziel's @jezieldamasceno.bsky.social recent paper on R-loops, DNA replication and genomic stability in Leishmania major www.nature.com/articles/s41... in @naturecomms.bsky.social @rmc9z.bsky.social @marquescata.bsky.social

R-loops acted on by RNase H1 influence DNA replication timing and genome stability in Leishmania - Nature Communications

All genomes are replicated in a temporal order, though the determinants of such timing are poorly characterized. Here, the authors show that the unusual chromosome size-dependent DNA replication timin...

nature.com

Please enjoy a new paper led by @jezieldamasceno.bsky.social that reveals how R-loops and their homeostatsis through RNase H1 modulate Leishmania DNA replication timing @uofgsii.bsky.social @marquescata.bsky.social @scotomorph.bsky.social www.nature.com/articles/s41...

R-loops acted on by RNase H1 influence DNA replication timing and genome stability in Leishmania - Nature Communications

All genomes are replicated in a temporal order, though the determinants of such timing are poorly characterized. Here, the authors show that the unusual chromosome size-dependent DNA replication timin...

nature.com

Excited to share our latest work on R-loops and RNAse H1, exploring their roles in genome replication timing and stability. It’s now published! www.nature.com/articles/s41... @rmc9z.bsky.social @marquescata.bsky.social @scotomorph.bsky.social

Fancy a sneak peek into Leishmania’s unusual DNA replication programming? At the McCulloch’s group we used D-Nascent to map origins & forks at the single-molecule level, revealing novel, abundant replication initiation events across its plastic genome. See links below👇