One of my favorite takeaways from our new paper: Embryonic cardiomyocytes aren't just small adult cardiomyocytes. During a brief developmental window they actively shape the ventricles. We show PKN2 is essential during this period, but largely dispensable afterward. rdcu.be/fx4jG

A narrow developmental window defines PKN2’s essential role in ventricular chamber morphogenesis

Communications Biology - Multi-omic and quantitative morphometric analyses define a narrow early developmental window during which cardiomyocytes require PKN2 for ventricular chamber morphogenesis...

rdcu.be

Quoting Minny from The Help: 'Frying chicken just tend to make you feel better about life.' Well, for me that would be protein purification.

I made Conway–Maxwell–Binomial regression on #TMB + #glmmTMB. A bounded-count family that handles both over- and under-dispersion through one parameter.. Demo on coral fertilization data from @benoitpujol.bsky.social #rstats #STEM #biology jbogomolovas2.github.io/Julius-s-Blo...

Conway–Maxwell–Binomial regression: two-directional dispersion for bounded counts – Julius’s Blog

Data Science, Swimming Analytics, and R Programming

jbogomolovas2.github.io

If you need to compare numbers of proliferating cells, ditch the t-test and go for count regression. You're more likely to get p < 0.05 when a real difference is there. I crunched the numbers: #rstats #statistics #biostats #ScienceSky #AcademicSky #biology jbogomolovas2.github.io/Julius-s-Blo...

Conway–Maxwell–Poisson vs Welch’s t-test for proliferation count data – Julius’s Blog

Data Science, Swimming Analytics, and R Programming

jbogomolovas2.github.io

Hierarchical Bayesian models are easier than they sound. I used one to ask whether some Lithuanian news outlets are more negative than others. The naive answer was wrong; partial pooling fixed it. brms + ordinal regression on 4,832 headlines. jbogomolovas2.github.io/Julius-s-Blo...

“And I’m looking for the funnies”: sentiment in Lithuanian news headlines – Julius’s Blog

Data Science, Swimming Analytics, and R Programming

jbogomolovas2.github.io

Looking for a book that covers both statistics and R programming? Check out my book Modern Statistics with R! Coding, visualisation, regression, ML & more. 📖 Read it for free: www.modernstatisticswithr.com 📚 Get printed copies: tinyurl.com/mswrbook #Rstats #statistics #datascience #machinelearning

Your weight has a weekly cycle and a yearly cycle. 3 years of daily weigh-ins confirm it: Monday heaviest → Thursday lightest January heaviest → September lightest New blog post breaking it all down with GAMs in R: jbogomolovas2.github.io/Julius-s-Blog/posts/weight_fluctations/ #rstats #datascience

Goals for my blog - a mini-series on the microbiome - recap of my experience with NLP - posting my posit:conf experience as a virtual participant (I have.....one post drafted and I think I may have been over ambitious 🥲)

Best way to learn something is to use it on a problem you care about. I've been getting into GAMs, so I built a swim base pace tracker — something #Garmin doesn't offer. #rstats #dataviz #swimming jbogomolovas2.github.io/Julius-s-Blog/posts/swim-css-pipeline/

Our latest paper is out. We show that smaller is sometimes better- by trimming out a large portion of the #ALPK3 kinase we create a functional miniALPK3 that fits into an AAV vector and treats ALPK3 cardiomyopathy. doi.org/10.1161/CIRC...

It was fun distraction to participate International Cherry Blossom Prediction Competition organized by @georgemasonu.bsky.social. Not the winner, but not shabby for a first try. jbogomolovas2.github.io/Julius-s-Blo...

Cherry Bloom Prediction – Julius’s Blog

Data Science, Swimming Analytics, and R Programming

jbogomolovas2.github.io

Our new paper in @commsbio.bsky.social. We show that Tnnt2 intronic RNAscope probe reliably identifies cardiomyocytes —even through mitosis and nuclear envelope breakdown—overcoming the limitations of traditional CM identification methods. www.nature.com/articles/s42...

Intronic RNAscope probes enable precise identification of cardiomyocyte nuclei and cell cycle activity - Communications Biology

Intronic RNA probes precisely identify cardiomyocyte nuclei or chromatin throughout the cell cycle during embryonic cardiomyocyte proliferation and precisely identify cardiomyocyte nuclei in adult hea...

nature.com

Our new paper in @commsbio.bsky.social. We show that Tnnt2 intronic RNAscope probe reliably identifies cardiomyocytes —even through mitosis and nuclear envelope breakdown—overcoming the limitations of traditional CM identification methods. www.nature.com/articles/s42...

Intronic RNAscope probes enable precise identification of cardiomyocyte nuclei and cell cycle activity - Communications Biology

Intronic RNA probes precisely identify cardiomyocyte nuclei or chromatin throughout the cell cycle during embryonic cardiomyocyte proliferation and precisely identify cardiomyocyte nuclei in adult hea...

nature.com

Our paper on #ALPK3 is out in JAHA. We demonstrate that loss of ALPK3 and established factor for genetic cardiomyopathy leads to accumulation of thick filament components, possibly via MuRFs - muscle specific E3 ubiquitin ligases. doi.org/10.1161/JAHA...

α Protein Kinase 3 Is Essential for Neonatal and Adult Cardiac Function | Journal of the American Heart Association

doi.org

If it ain't green and glow in the dark it ain’t #science. Leftovers from #mClover3 fluorescent protein purification

New work on how and when Indo-europeans emerged. Nice to see how Marijas Gimbutas Kurgan hypothesis has been verified using ancient DNA approach and new insights and details obtained.

Iosif Lazaridis @iosiflazaridis.bsky.social · last yr.

Our papers, out today in @nature.com, show how ancient DNA from the Eneolithic and Bronze Age steppe points to a North Pontic origin of the Indo-European language family and a Caucasus-Lower Volga (CLV) origin of Indo-Anatolian (inclusive of the now extinct Anatolian languages). 1/

Very likely successful cloning of HDR template for knock-in (ccdB present in source plasmid, sequencing pending). Still got the cloning touch! 🧬 #KeepCalmAndCarryOn

Hey Bluesky! If you're an Addgene fan, you might want to sign up for our quarterly newsletter. Each edition contains the latest Addgene news, hot plasmids, new viral vectors, and a coupon. Click the link below to browse old editions and join our mailing list! www.addgene.org/news...

Small but mighty .🐦‍🔥

Excited to share our new paper on a PKN biosensor—and how to build more! We only have a handful of kinase biosensors out there; now that ExRai backbone @jinzhanglab “anything you can do I can do better” for excising sensors real bottleneck is finding new substrates. go.nature.com/42rUgpO

Illuminating understudied kinases: a generalizable biosensor development method applied to protein kinase N - Communications Biology

Development of genetically encoded fluorescent biosensor allows real-time visualization of PKN kinase activity, revealing sustained activity at the plasma membrane and demonstrating a new approach for...

go.nature.com