Check out our new preprint! We describe conformation-specific nanobodies that allow us to study Guanylate-binding proteins and target different stages of their assembly. We shed some new light on current models and hypotheses!

Arjen Jakobi@cryotud.bsky.social · 4w ago

State-selective nanobodies to probe Guanylate-binding protein coat assembly and function Lennart & Tanja identified and validated conformation-selective nanobodies to probe and interfere w/ GBP coat formation on intracellular bacteria. Led by @lpagani.bsky.social. www.biorxiv.org/content/10.6...

An Asgard archaeon with internal membrane compartments Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors! www.biorxiv.org/content/10.1...

Really cool work Wout and colleagues! @raunser-lab.bsky.social presented this work a couple of weeks ago at the Dutch Structural Biology meeting - I was really impressed.

Wout Oosterheert@woutoosterheert.bsky.social · last yr.

Very excited that my final postdoctoral work is now online in Cell @cellpress.bsky.social. www.sciencedirect.com/science/arti... Using 16 (!) cryo-EM structures, we uncovered how the three proteins coronin, cofilin and AIP1 work together to rapidly disassemble actin filaments.

Thrilled to announce that from tomorrow (October 1st), I'll start as a junior group leader at the Netherlands Cancer Institute (NKI) @nkinl.bsky.social in Amsterdam 😄. www.nki.nl/news-events/... 1/3

We finally wrote up the paper for LocScale-2.0. edu.nl/pvhxv Please try it out. LocScale is evolving & we are actively building in new features and improvements. Your feedback will help us shape the next steps. Code: cryotud.github.io/locscale/ Work by @alok-bharadwaj.bsky.social and Reinier.

Arjen Jakobi@cryotud.bsky.social · last yr.

We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation. Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation. cryotud.github.io/locscale/

Together with colleagues at the Dept. of Microsystems Engineering, we are looking for a PhD student to develop methods for femtoliter-scale sample dispensing in cryoEM sample preparation. edu.nl/h436k If you like interdisciplinary research & fascinating physics at ultrasmall scales, please apply!

Targeted disruption of phage liquid crystalline droplets abolishes antibiotic tolerance of bacterial biofilms led by @abultarafder.bsky.social Great collaboration with @pearce-maths.bsky.social and @geiselbiofilm.bsky.social www.biorxiv.org/cgi/content/...