This was a great sample to check out -- who knew 100M year old amber would be so close to Ethyl Cinnamate in RI? Huge shout out to @brukercorporation.bsky.social for the fleet of lightsheets at @lsfm-na.bsky.social last week!

New England Society for Microscopy@nesmicroscopy.bsky.social · last yr.

For #MicroscopyMonday, a 100M year-old 🦟 in amber! Lots of familiar #NESM faces up in Acadia for the #LSFM25 at the MDI @mdibiolab.bsky.social ! 🔬: Bruker @brukercorporation.bsky.social, LCS SPIM 🟠: Courtesy the Sims Collection 🥼&🎥: Jan Frankowski @frankowskijc.bsky.social @lsfm-na.bsky.social

For #MicroscopyMonday, a 100M year-old 🦟 in amber! Lots of familiar #NESM faces up in Acadia for the #LSFM25 at the MDI @mdibiolab.bsky.social ! 🔬: Bruker @brukercorporation.bsky.social, LCS SPIM 🟠: Courtesy the Sims Collection 🥼&🎥: Jan Frankowski @frankowskijc.bsky.social @lsfm-na.bsky.social

And with that, #lsfm25 comes to a close! We want to thank everyone who helped make this possible - the faculty, course directors, TAs, vendors, and sponsors. But most importantly the many wonderful people at MDIBL. See you all next year!

Preparation for the final event of the course: a boat tour of Acadia!

Now that the students have had several days to learn sample preparation and image acquisition, we are wrapping up day five with a crash course called "Now what do you do with your data?" Led by @dpshepherd.bsky.social and Carsten Wolff. #lsfm25 #microscopy #science #education

Spending some time at #lsfm25 cleaning up code. It now has distributed GPU gradient-consensus RL decon (@jamesdmanton.bsky.social @andrewgyork.bsky.social) our fast orthogonal deskew, and stitching/fusion to ome-ngff v0.5. Example AO-OPM data on uncleared mouse brain slice github.com/QI2lab/opm-p...

Up in Acadia at the @mdibiolab.bsky.social for #LSFM25! Here's a cleared whole mouse head on the SmartSPIM, 3.6X objective for #3DThursday. 🔎 @jtakatoh.bsky.social, now at @stonybrooku.bsky.social 🔬 LifeCanvas Technologies, lifecanvastech.com/products/sma... 🥽 syGlass.io @lsfm-na.bsky.social

We are so excited to start day four with a wonderful talk from Kara Cerveny! #lsfm25 #science #microscopy #education @mdibiolab.bsky.social @kara-cerveny.bsky.social

It's day three and we are starting our morning off with an excellent intro to clearing by Doug Richardson. #lsfm25 #microscopy #science #education @mdibiolab.bsky.social

And it begins....

The calm before the storm indeed! In just a half an hour, this board will be bombarded by 25 students rushing to grab a spot on their instrument of choice. 11 systems. Only 9 labs. May the odds be ever in their favor... #lsfm25 #microscopy #science #education @mdibiolab.bsky.social

Light-Sheet Fluorescence Microscopy (LSFM) course@lsfm-na.bsky.social · last yr.

Calm before the storm…

We are back from lunch with a talk from Raghav Chhetri telling us all about using light sheet microscopy for neuroscience imaging! #lsfm25 #microscopy #science #education @mdibiolab.bsky.social @rchhmiscope.bsky.social

It's day two! We're starting off a day filled with science with a talk from Anna-Karin Gustavsson from Rice University. #lsfm25 #microscopy #science #education @mdibiolab.bsky.social @riceuniversity.bsky.social @gustavssonlab.bsky.social

Morning in Bar Harbor doesn’t suck.

Step 8: In @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Well day one done worth a sunset!

Step @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Went to collect some samples... Here is a pink tunic ate!

Reto Fiolka and OPM for the Keynote!

With the end of our first round of vendor talks behind us, we are excited to welcome Reto Fiolka as our keynote speaker this year. #lsfm25 #microscopy #science #education @mdibiolab.bsky.social

Holly Gibbs discussing the logistics of image processing and analysis

While we are on our lunch break, the students are enjoying some beautiful weather here at @mdibiolab.bsky.social. #lsfm25 #microscopy #science #education

We now have Vera Morales from Nikon telling us all about objectives: the why, when, and how! #lsfm25 #microscopy #science #education

Claudette St Croix from CBI kicks off LSFM 2025 at @mdibiolab.bsky.social

@lsfm-na.bsky.social Is this heaven, or am I just currently surrounded by ELEVEN DIFFERENT LIGHT SHEET MICROSCOPES? So thrilled to be out here in Acadia chatting about microscopy!

LSFM 2025 has kicked off with an introduction from the one and only, @glaevsky.bsky.social, our course director. #lsfm25 #microscopy #science #education

Calm before the storm…

Time to sheet!

Emmanuel G. Reynaud@ereynaud.bsky.social · last yr.

Step 5: On my way to @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Arrived, slept and enjoying the Acadia Channel!

Why no swim?!

Emmanuel G. Reynaud@ereynaud.bsky.social · last yr.

On my way to @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Could not swim to it this year so start from Mt St Michel. See you in a bit...

Step 2 : On my way to @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Queueing and sterile halls is next at Charles de Gaulle Airport !

See you soon!

Emmanuel G. Reynaud@ereynaud.bsky.social · last yr.

Step 3 : On my way to @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Made it over the pond. Boston here I am!

Registration prices are going down but quality will be the same!

MDI Biological Laboratory@mdibl.org · last yr.

Have you been thinking of enrolling the MDI Bio Lab's #LightSheet Fluorescence Microscopy course? Registration has been lowered to $1,000 — down from $3,500! This is a great opportunity for researchers/students to enhance their knowledge & skills in cutting-edge #microscopy techniques. 🧪 🤝 🍎 👩‍🔬