Tessera
Folgen

Anna-Karin Gustavsson

@gustavssonlab.bsky.social

Develops single-molecule imaging tools to gain detailed information about cellular nanoscale structures, dynamics, and molecular mechanisms. https://gustavssonlab.rice.edu

41 Posts1173 Follower395 Folgt
Anna-Karin Gustavsson@gustavssonlab.bsky.social · 3mo ago

Excited that our work on versatile and scalable micromirror inserts that enable high-contrast and high-resolution single-objective light sheet microscopy in conventional sample chambers is now published in Nano Letters! doi.org/10.1021/acs.... @pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale

Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy

We present a tunable microfabrication pipeline for creating robust, reflective inserts that adapt conventional commercial imaging chambers for single-objective light sheet (LS) illumination. This system reduces the complexity associated with dual-objective LS setups and specialized LS chambers while retaining the native functionality and biocompatibility of the original chambers. The fabricated insert features a metalized, 3D nanoprinted micromirror with an angled reflective surface, enabling alignment of a thin LS for sectioning and imaging throughout mammalian cells. Using this pipeline, we demonstrate that single-objective LS illumination achieves an over 4X improvement in the signal-to-background ratio compared with conventional widefield epi-illumination in both fixed and live cell samples. Furthermore, we show substantial resolution enhancement for single-molecule localization microscopy compared to epi-illumination for improved imaging at the nanoscale. The versatile and scalable design offers an easily implemented approach to bring the benefits of single-objective LS microscopy to a wide array of biological studies.

doi.org

Anna-Karin Gustavsson@gustavssonlab.bsky.social · 9mo ago

Excited that our work on Single-objective lattice light sheet microscopy with microfluidics for single-molecule super-resolution imaging of mammalian cells is now published in ACS Photonics! doi.org/10.1021/acsp... @pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale #imaging

Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells

Single-molecule localization microscopy (SMLM) has redefined optical imaging by enabling imaging beyond the diffraction limit, allowing for nanoscale investigation into cellular architecture and molec...

doi.org

Anna-Karin Gustavsson@gustavssonlab.bsky.social · 2y ago

Work lead by our collaborators in the Hilton Lab on how biomolecular condensation of human IDRs initiates endogenous transcription via intrachromosomal looping or high-density promoter localization is now published in @narjournal.bsky.social as a Breakthrough Article! doi.org/10.1093/nar/...

Biomolecular condensation of human IDRs initiates endogenous transcription via intrachromosomal looping or high-density promoter localization

Abstract. Protein intrinsically disordered regions (IDRs) are critical gene-regulatory components and aberrant fusions between IDRs and DNA-binding/chromat

doi.org

Nucleic Acids Research@narjournal.bsky.social · 2y ago

New NAR #Breakthrough Article from the Hilton lab "Biomolecular condensation of human IDRs initiates endogenous transcription via intrachromosomal looping or high-density promoter localization" 👉 Read the full article here: https://doi.org/10.1093/nar/gkaf056

Anna-Karin Gustavsson@gustavssonlab.bsky.social · 2y ago

Excited that our work on Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet (soTILT3D) is now published in Nature Communications! doi.org/10.1038/s414... @natureportfolio.bsky.social #microscopy #imaging #nanoscale

Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet - Nature Communications

Super-resolution imaging of whole mammalian cells is often hampered by high fluorescence background and slow acquisition speeds. Here, the authors present soTILT3D, a platform based on a steerable sin...

doi.org

Anna-Karin Gustavsson@gustavssonlab.bsky.social · 2y ago

Excited that our work on scan-free whole-cell 3D single-molecule super-resolution imaging with simplified and improved experimental and analysis workflows using long-axial-range double-helix point spread functions is now published! doi.org/10.1021/acs.... @gustavssonlab.bsky.social, @ShechtmanLab

Long-Axial-Range Double-Helix Point Spread Functions for 3D Volumetric Super-Resolution Imaging

Single-molecule localization microscopy (SMLM) is a powerful tool for observing structures beyond the diffraction limit of light. Combining SMLM with engineered point spread functions (PSFs) enables 3...

doi.org