In DNA-PAINT, nearby proteins create overlapping localizations. You may see two by eye, but analysis often misses them, wasting precious precision. G5M solves this. Now in @natcomms.nature.com. doi.org/10.1038/s414.... 1/7

Molecular mapping in DNA-PAINT via modified Gaussian Mixture Modeling - Nature Communications

Standard algorithms fail to fully exploit the spatial information in DNA-PAINT. Here, the authors present G5M, an algorithm providing molecular maps by accurately inferring biomolecule positions at di...

doi.org

Honoured to receive the Young Fluorescence Investigator Award at #BPS2026! Huge thanks to the selection committee and to all the mentors and colleagues who supported me. Excited to start new projects and keep using fluorescence to uncover molecular biology!

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells. www.nature.com/articles/s41... rdcu.be/eQVxt

4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods

4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

nature.com

We're excited to announce the poster prize winners of #EESImaging 🔬 A special poster prize committee elected three winners: Alara Kiris, @lumasullo.bsky.social and @wanluzhang.bsky.social. Congratulations! Read on to learn about their research: s.embl.org/ees25-09post...

Now online: October 2025 Issue. - Focus Issue on #biosensing, - DNA moiré superlattices, - Sugars at Ångström-resolution, - Solid-state #nanopores, - Non-aqueous Li #batteries, - - Neuromorphic vision, - Peptide #hydrogels, - Deep learning for #LNPs and more... www.nature.com/nnano/volume...

Our article "Ångström-resolution imaging of cell-surface glycans" made it to the cover of Nature Nanotechnology! 🤩 @natnano.nature.com www.nature.com/nnano/volume...

Thrilled to announce some big news! Excited to share that I’ve been awarded a Wellcome Trust Early Career Award to establish my research as a Group Leader in the Glycosciences Program at the Institute of Biology and Experimental Medicine (IBYME) in Buenos Aires, Argentina, beginning in early 2026.

Within this neuronal atlas we can reveal the three synapse classes, excitatory, inhibitory and the recently discovered mixed synapse. Organelle imaging of Peroxisomes (Pmp70) and the Golgi Apparatus (Golga5) reveals rare contact sides and even fused particles. (5/6)

To show the power of the technique, we acquired a 13-plex 200 x 200 µm2 neuronal atlas in 3D. With this atlas we map the interaction architecture of three neurons, resolving organelles, cytoskeleton, vesicles and synapses at single-protein resolution. (4/6)

We demonstrate speed-optimized left-handed DNA-PAINT by characterizing the sequence binding kinetics and resolving three main microscopy benchmarking targets, mitochondria, microtubules and nuclear pore complexes with <5 nm localization precision. (3/6)

The mirrored design of left-handed oligonucleotides allows the extension of the common 6 speed-sequences R1-R6 with their analogs L1-L6, enabling 12 target multiplexing with a standard secondary label-free DNA-PAINT workflow. (2/6)

Highly efficient 12-color multiplexing with speed-optimized DNA-PAINT. We are excited to share our latest paper in @natcomms.nature.com, using left-handed DNA to extend speed-optimized DNA-PAINT to 12 targets in a simple and straightforward way! 🧬👈🚀https://www.nature.com/articles/s41467-025-64228-x

📣 📅 Invitation to two talks at our institute next Tuesday, October 7: 🤩 Luciano Masullo @lumasullo.bsky.social from @mpibiochem.bsky.social at 10 am, Adam Cohen @harvard.edu at 3pm, also online: us06web.zoom.us/webinar/regi... #maxplanck #mpimr #colloquium #medicalresearch #sciencetalks #science

Happy to share this article with my views on @elislenders.bsky.social and @vicidominilab.bsky.social work and discussing current developments in single-molecule localization combining structured excitation and detection. So many exciting perspectives in the field! www.nature.com/articles/s41...

Localization of single molecules with structured illumination and structured detection - Light: Science & Applications

Light: Science & Applications - Localization of single molecules with structured illumination and structured detection

nature.com

Ångström-resolution imaging of cell-surface glycans Now published in Nature Nanotechnology! 🤩 @natnano.nature.com www.nature.com/articles/s41...

Ångström-resolution imaging of cell-surface glycans - Nature Nanotechnology

By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...

nature.com

Luciano A. Masullo@lumasullo.bsky.social · last yr.

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...

New paper online: Ångström-resolution imaging of cell-surface glycans. The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes. #Glycotime www.nature.com/articles/s41...

Ångström-resolution imaging of cell-surface glycans - Nature Nanotechnology

By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...

nature.com

Congratulations to everyone involved: @ipachmayr.bsky.social, @lumasullo.bsky.social, @susannereinhardt.bsky.social, Jisoo Kwon, Ondřej Skořepa, Maite Llop, Sylvia Herter, Marina Bacac and Christian Klein. (6/6) Read the full story here: www.nature.com/articles/s41...

Resolving the structural basis of therapeutic antibody function in cancer immunotherapy with RESI - Nature Communications

The nanoscale organization of the antigen-antibody complexes influences the therapeutic action of monoclonal antibodies. Here, the authors present a multi-target 3D RESI imaging assay for the nanomete...

nature.com

Can we break past the usual limits of #SuperResolution #Microscopy—achieving deep-imaging, high-res, large FOV imaging without the trade-offs? 🧬🔬 YES: with #DNA_PAINT + Spinning Disk Confocal + Optical Photon Reassignment (#SDC-OPR)! 📄 www.nature.com/articles/s41... @natcomms.nature.com! 🧵👇

Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment - Nature Communications

Zaza and colleagues demonstrate that DNA-PAINT on a spinning disk confocal microscope with optical photon reassignment enables high-resolution imaging across large fields and imaging depths, resolving...

nature.com

A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊 📄 bit.ly/4jTHfes

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications

Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

bit.ly

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩 We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

JungmannLab@jungmannlab.bsky.social · last yr.

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution We are excited to present our latest work published in @natcomms.nature.com www.nature.com/articles/s41...

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution We are excited to present our latest work published in @natcomms.nature.com www.nature.com/articles/s41...

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications

Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

nature.com

To look into: a Python alternative to ZEMAX... github.com/HarrisonKram...

GitHub - HarrisonKramer/optiland: Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch.

Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch. - HarrisonKramer/optiland

github.com

I had a blast visiting the lab of Prof. Peng Xi at Peking University!! Prof. Xi and his team are doing amazing work on various type of SIM and other imaging modalities! Thanks so much for the warm welcome, the stimulating discussions, and the campus tour!!

Amazing visit to the lab of Prof. Wei Ji at the Institute of Biophysics (CAS) in Beijing today! They’re doing top-notch research, including awesome interferometric single-molecule localization methods (ROSE) thanks so much for the invitation, warm welcome and hospitality!! 😍🤩

#FOM2025 in Taiwan starting now!! Looking forward to great talks and also to tell about RESI and our latest work!

IMAGING LIGAND-RECEPTOR INTERACTIONS AT SINGLE-PROTEIN RESOLUTION WITH DNA-PAINT🔬 Ever wonder how cells "talk"? It starts when ligands bind to receptors on cell surfaces. We have cracked the challenge of imaging small ligands on cell surfaces. #DNAPAINT #celltalk 💬 doi.org/10.1002/smtd...

Check-out this powerful application of DNA-PAINT and RESI: imaging ligand-receptor interactions at molecular resolution! Project led by my extremely talented colleagues Monique Honsa, Larissa Heinze and Isabelle Pachmayr Very happy to have contributed to this beautiful work! 🤩

Monique Honsa@moniquehonsa.bsky.social · last yr.

IMAGING LIGAND-RECEPTOR INTERACTIONS AT SINGLE-PROTEIN RESOLUTION WITH DNA-PAINT🔬 Ever wonder how cells "talk"? It starts when ligands bind to receptors on cell surfaces. We have cracked the challenge of imaging small ligands on cell surfaces. #DNAPAINT #celltalk 💬 doi.org/10.1002/smtd...

One month ago today, I published my first paper with the @jungmannlab.bsky.social 🥳 What better way to celebrate #MicroscopyMonday than with this STAR Protocol on SUM-PAINT spatial proteomic imaging: a guide for highly multiplexed DNA-PAINT imaging in neurons. star-protocols.cell.com/protocols/4066

Cell Press: STAR Protocols

STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

star-protocols.cell.com

An interesting preprint from the Horrocks' lab detailing the behavior of secondary nanobodies (such a @nanotag.bsky.social ones) at the single molecule level and optimizing protocols for multiplexed labeling: doi.org/10.1101/2025...

Mike spotted this before the rest of us. 🫡 Publications from 2025 are shared more on Bluesky than on X/Twitter.

@herrison2000.bsky.social · last yr.

About to talk to medical affairs colleagues, with the news that Bluesky is carrying more posts about new journal publications than X #bluesky #altmetrics #x #xparrot