NanoTag Biotechnologies

@nanotag.bsky.social

𝐂𝐮𝐬𝐭𝐨𝐦 𝐒𝐢𝐧𝐠𝐥𝐞-𝐃𝐨𝐦𝐚𝐢𝐧 𝐀𝐧𝐭𝐢𝐛𝐨𝐝𝐲 𝐃𝐢𝐬𝐜𝐨𝐯𝐞𝐫𝐲 & 𝐍𝐚𝐧𝐨𝐛𝐨𝐝𝐲-𝐁𝐚𝐬𝐞𝐝 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐓𝐨𝐨𝐥𝐬

Congratulations @syncellbiolab.bsky.social team on this impressive work! 👏 It's exciting to see IntAct-U-ExM revealing actin architectures with such remarkable detail. Happy to see our #ALFAsystem enabling robust post-expansion labeling & high-resolution visualization of complex actin networks. 🦙🔬

Saravanan Palani@syncellbiolab.bsky.social · 2mo ago

Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by @anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...

📄🔬 Congratulations to all authors on this excellent preprint showcasing #NanoFLex, a FLIM strategy that enables lifetime-based separation of multiple targets in a OneStep-IF using our #SmartSecondaries fused to different #HaloTag variants. 👏 We’re proud that SmartSecondaries® power this workflow☺️

Felipe Opazo@opazo.bsky.social · 5mo ago

New preprint out! In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

Did you know #multiplex IF can be done in just one incubation step? ❌ No sequential staining ❌ No host-species limits ❌ No secondary-induced clustering 🔬Pre-mix your favourite primary antibodies (even same-species) with Smart Secondaries® #nanobody & apply directly to your sample for OneStep IF.🤩

Neuroscientists - looking for a high-affinity gift?💝 Inside the postsynapse, proteins crowd the show PSD95’s the star - but hard to know. IgG antibodies knock, but can’t get through Too bulky to reach the target they pursue. But FluoTag®-X2 anti-PSD95 slips right in Binds PSD95. Precise within.

📄This new Journal of Microscopy 🔬 paper uses rigorous single-molecule assays to benchmark secondary nanobodies and shows NanoTag #SmartSecondaries® deliver high-affinity binding, efficient saturation, and exceptionally low off-target labeling for robust #multiplexed immunoassays 💪

@jofmicroscopy.bsky.social · 6mo ago

🔬 In this #OpenAccess paper, the authors propose a set of experiments spanning total internal reflection fluorescence and confocal microscopies that can be used to validate secondary nanobody binding, specificity, and their propensity for mis-targeted binding in multiplex assays.