This story is now published in @natplants.nature.com ! 🎉🎩 Check out the final article here: nature.com/articles/s41477-026-02361-1 Very similar to the original bioRxiv preprint, with the addition of some cool polysome analysis (in the supplementals)! Thanks and congrats to everyone involved! 🙏

Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome - Nature Plants

Using in situ cryo-electron tomography, researchers found a unique ‘arm’ domain on the small subunit of Chlamydomonas chlororibosomes. This discovery suggests that chlororibosome structure is more evo...

nature.com

Florent Waltz@florentwaltz.bsky.social · 6mo ago

Long in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵

1/7 New preprint: we found that Chlamydomonas carries a giant and unexpectedly elaborate chloroplast RNA polymerase with 12 previously unknown subunits, overturning the idea that the complexity of this organellar enzyme emerged during plant terrestrialization! www.biorxiv.org/content/10.6...

The structure of a 2-MDa chloroplast RNA polymerase reveals unexpected evolutionary complexity

Transcription in chloroplasts depends on the Plastid-Encoded RNA polymerase (PEP), a bacterial-derived enzyme whose catalytic core remains encoded by the highly reduced genome inherited from the cyano...

biorxiv.org

It’s a pyrenoid party! 🪩🌍 Awesome collab🤝 of @phaips.vd.st & @manondemulder.bsky.social with Mihris and the @mackinderlab.bsky.social crew to reveal the molecular architecture🏗️ of the Chorella pyrenoid. Algae have so many convergently evolved compartments to supercharge #CO2 capture. #TeamTomo 🔬🌾🌊🧪

Mihris Naduthodi@mihrisn.bsky.social · 2w ago

(1/16) Nearly two-thirds of ocean carbon fixation flows through a diverse organelle: the pyrenoid. Our new paper maps its 3D architecture in the alga Chlorella and identifies new proteins — expanding the toolkit to engineer this CO2-concentrating machine into crops🧵 #Pyrenoid #CCM #Photosynthesis

Another day, another cool paper from @mackinderlab.bsky.social @james-r-barrett.bsky.social www.biorxiv.org/content/10.6...

Molecular organization of the Chlorella sorokiniana pyrenoid

To overcome the enzymatic limitations of Rubisco, algae operate CO2-concentrating mechanisms (CCMs) that deliver concentrated CO2 to Rubisco tightly packaged in a specialized microcompartment called a...

biorxiv.org

(1/16) Nearly two-thirds of ocean carbon fixation flows through a diverse organelle: the pyrenoid. Our new paper maps its 3D architecture in the alga Chlorella and identifies new proteins — expanding the toolkit to engineer this CO2-concentrating machine into crops🧵 #Pyrenoid #CCM #Photosynthesis

Another pyrenoid, another example of convergent evolution to condense Rubisco and turbocharge photosynthesis. This time in a multicellular organism. So much fun exploring a seaweed pyrenoid with @jonasblomme.bsky.social, @mjplevin.bsky.social, @jnb-lab.bsky.social, @james-r-barrett.bsky.social.

Jonas Blomme@jonasblomme.bsky.social · 3w ago

Excited to share our latest preprint on #PhycologyFriday. We map out the carbon fixation pathway for the first time in a multicellular alga, the green seaweed Ulva, and reveal a unique Rubisco-Linker interaction in the pyrenoid, a chloroplast condensate. Below I summarize some key points. #seaweeds

Check out this cool thread on the latest preprint by Gaurav Kumar, @james-r-barrett.bsky.social, @phaips.vd.st from our lab and colleagues, dissecting the physics of Rubisco condensates! You're in for some stunning #cryoET of the pyrenoid - in Chlamy, of course 😉 Congrats to all involved!

James Barrett@james-r-barrett.bsky.social · 6mo ago

How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid

Check this new study on carbon concentration mechanisms! See how the pyrenoid changes by modifying Rubisco linkers in our favorite alga, Chlamy 💚! My desk mate @phaips.vd.st spent a lot of time collecting & carefully analyzing these beautiful tomograms 🔬. Congrats to all the authors!

James Barrett@james-r-barrett.bsky.social · 6mo ago

How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid

That is a really beautiful poster!🤩 🧪 Inside the Pyrenoid: How Multivalency Shapes Rubisco Organization and CO2 Fixation www.biorxiv.org/content/10.6...

James Barrett@james-r-barrett.bsky.social · 6mo ago

Lastly, if you prefer to see this as a poster, you can find that here: james-r-barrett.github.io/presentation...

You like #LLPS? #TeamTomo? #Rubisco? Check out our latest preprint! What a great collaboration this has been!

James Barrett@james-r-barrett.bsky.social · 6mo ago

How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid

How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid