Why do intrinsically disordered proteins behave so weirdly during SDS-PAGE? This is the question we aim to answer in our recent paper: onlinelibrary.wiley.com/doi/10.1002/...

Sequence determinants of the hypomobility of intrinsically disordered proteins in <fc>SDS</fc>‐<fc>PAGE</fc>

Proteins with intrinsically disordered regions (IDRs) migrate at a higher apparent molecular weight in sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, complicating their analysis and...

onlinelibrary.wiley.com

🚀 New preprint 🥳 Fantastic work by our recent PhD graduate Daan ! How are mRNPs remodeled as they exit the nucleus? We identify PAIP1 as a factor that promotes exchange of the nuclear poly(A)-binding protein PABPN1 for cytoplasmic PABPC1, coupling mRNA export to cytoplasmic mRNP maturation.

bioRxiv Molecular Biology@biorxiv-molbio.bsky.social · 2d ago

PAIP1 couples mRNA export to cytoplasmic mRNP remodeling and poly(A) homeostasis https://www.biorxiv.org/content/10.64898/2026.07.31.742013v1

The artist formerly known as #thatmitoriboguy has now become #thatchlororiboguy Check out some cool tomo from @florentwaltz.bsky.social @phaips.vd.st

Florent Waltz@florentwaltz.bsky.social · last wk.

This story is now published in @natplants.nature.com ! 🎉🎩 Check out the final article here: nature.com/articles/s41477-026-02361-1 Very similar to the original bioRxiv preprint, with the addition of some cool polysome analysis (in the supplementals)! Thanks and congrats to everyone involved! 🙏

And 2 complementary 📜: 1️⃣ Cyanobacterial & vascular plant chlororibos🌿 (by @michaelwebster.bsky.social, with contrib from @sofie-dot-rec.bsky.social, @cellarchlab.com & I): biorxiv.org/content/10.64898/2026.07.03.736309v1 2️⃣ Marchantia chlororibos 🥬: biorxiv.org/content/10.64898/2026.07.14.738477v2

This story is now published in @natplants.nature.com ! 🎉🎩 Check out the final article here: nature.com/articles/s41477-026-02361-1 Very similar to the original bioRxiv preprint, with the addition of some cool polysome analysis (in the supplementals)! Thanks and congrats to everyone involved! 🙏

Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome - Nature Plants

Using in situ cryo-electron tomography, researchers found a unique ‘arm’ domain on the small subunit of Chlamydomonas chlororibosomes. This discovery suggests that chlororibosome structure is more evo...

nature.com

Florent Waltz@florentwaltz.bsky.social · 6mo ago

Long in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵

Regulatory logic of the Chlamydomonas CO2-concentrating mechanism: coupling carbon flux, energy supply, and pyrenoid architecture www.frontiersin.org/journals/pla...

Frontiers | Regulatory logic of the Chlamydomonas CO2-concentrating mechanism: coupling carbon flux, energy supply, and pyrenoid architecture

The algal CO2-concentrating mechanism (CCM) elevates CO2 around Rubisco, the CO2-fixing enzyme, through the coordinated action of inorganic carbon (Ci) uptak...

frontiersin.org

Regulatory logic of the Chlamydomonas CO2-concentrating mechanism: coupling carbon flux, energy supply, and pyrenoid architecture www.frontiersin.org/journals/pla...

Frontiers | Regulatory logic of the Chlamydomonas CO2-concentrating mechanism: coupling carbon flux, energy supply, and pyrenoid architecture

The algal CO2-concentrating mechanism (CCM) elevates CO2 around Rubisco, the CO2-fixing enzyme, through the coordinated action of inorganic carbon (Ci) uptak...

frontiersin.org

Engineering a pyrenoid into crops could boost yields, but testing components in plants takes months. To accelerate engineering, we made Nicotiana benthamiana with hybrid algal Rubisco, enabling the reconstitution of pyrenoid number regulation in a plant! www.biorxiv.org/content/10.6...

Check it out! Deeper insights into the pyrenoid of Chlorella. More evidence Rubisco has convergently evolved as an organising hub, cool membrane architectures and evidence that most of the rest of the CCM is conserved with other green pyrenoids! 💚 Preprint here 🔗: www.biorxiv.org/content/10.6... 🔗

Mihris Naduthodi@mihrisn.bsky.social · 2w ago

(1/16) Nearly two-thirds of ocean carbon fixation flows through a diverse organelle: the pyrenoid. Our new paper maps its 3D architecture in the alga Chlorella and identifies new proteins — expanding the toolkit to engineer this CO2-concentrating machine into crops🧵 #Pyrenoid #CCM #Photosynthesis

(3/16) What is a pyrenoid? It is a droplet-like compartment inside the chloroplast that packs Rubisco (the enzyme that fixes CO2) together with a CO2 delivery system, raising local CO2 around Rubisco and making carbon fixation faster and more efficient.

(1/16) Nearly two-thirds of ocean carbon fixation flows through a diverse organelle: the pyrenoid. Our new paper maps its 3D architecture in the alga Chlorella and identifies new proteins — expanding the toolkit to engineer this CO2-concentrating machine into crops🧵 #Pyrenoid #CCM #Photosynthesis

4/4 Our data supports the convergent evolution of a biomolecular condensate. We show that the main pyrenoid assembly protein, SUPA1, has minimal sequence conservation to characterized unicellular algal Rubisco-condensing proteins, binding to a different surface region of Rubisco.

3/4 We provide a unique insight into Rubisco condensation. We combine multi-discipline approaches to reveal the helical folding of Rubisco Binding Motifs upon binding, combined with steric hindrance that halves the available binding sites from eight to four.

Excited to share our latest preprint on #PhycologyFriday. We map out the carbon fixation pathway for the first time in a multicellular alga, the green seaweed Ulva, and reveal a unique Rubisco-Linker interaction in the pyrenoid, a chloroplast condensate. Below I summarize some key points. #seaweeds

bioRxiv Cell Biology@biorxiv-cellbio.bsky.social · 3w ago

A spatial atlas of the seaweed CO2-fixation machinery reveals a unique Rubisco condensation mechanism https://www.biorxiv.org/content/10.64898/2026.07.16.738900v1

First glimpses into the molecular kaleidoscope of pyrenoid diversity www.sciencedirect.com/science/arti...

First glimpses into the molecular kaleidoscope of pyrenoid diversity

A significant proportion of global photosynthetic carbon fixation relies on the pyrenoid, a biomolecular condensate found in the chloroplast of most u…

sciencedirect.com

AF-CALVADOS is now published doi.org/10.1002/pro.... We combine AlphaFold and CALVADOS to simulate flexible multidomain proteins at scale: — Ensembles of >12000 full-length human proteins — Comparison of IDRs alone and I n context for >1500 TFs @sobuelow.bsky.social @kejohansson.bsky.social

Excited to share my paper! 🥳 Today in #Nature we reveal an 8 MDa Hdr electron bifurcating super-assembly in class I methanogens that couples the first and last steps of hydrogenotrophic methanogenesis. A lineage-specific adaptation to anaerobic niches. www.nature.com/articles/s41...

Tired of AI-generated figures? I asked our talented scientific illustrator Manon Zuurmond to draw some of the instruments and lab equipment we missed in NIH's otherwise fantastic BioArt resource. First batch now available as CC BY. Free for everyone to use in publications: github.com/Center-for-P...

The algal pyrenoid turbocharges global CO2 fixation. Central to its function are specialized membranes that deliver CO2. We discovered that the protein RBMP2 makes these membranes—and found that the mysterious minitubules appear to enhance CO₂ delivery! www.biorxiv.org/content/10.6...

Mature plant chloroplasts form reversible gyroid cubic membranes www.biorxiv.org/content/10.6...

Mature plant chloroplasts form reversible gyroid cubic membranes

Across kingdoms, cells fold their membranes into precise shapes closely linked to their functions. In mature land-plant chloroplasts, the photosynthetic membranes have been viewed as strictly lamellar...

biorxiv.org

New Assistant (tenure-track) or Associate Professor position in Climate #Biophysics! Join our international research community to explore fundamental molecular mechanisms underlying climate-relevant biological processes. Apply by 7 Sept 2026! www.biozentrum.unibas.ch/de/open-posi...

A step forward in discovering Buchnera's key to the aphid's heart, published today! Thanks to Jerry Maeda and other authors--a great team -- and to the helpful reviewers #SymbioSky doi: doi.org/10.1038/s415...

A secreted endosymbiont protein essential for colonizing host cells - Nature

SyeA was present in the Buchnera ancestor, is secreted into the host cytoplasm, is homologous to secreted effectors of bacterial pathogens and is essential for Buchnera transmission.

doi.org

A versatile tool for gene editing in the diatom Thalassiosira pseudonana academic.oup.com/plphys/artic... @plantphys.bsky.social

A versatile tool for gene editing in the diatom Thalassiosira pseudonana

An integrated genetic toolkit enables systematic molecular investigation of ocean diatoms, microscopic algae that contribute approximately one-fifth of glo

academic.oup.com

From v0.2.0 MissAlignment now has an inference mode where you can apply the trained models from one dataset to a new dataset to skip training time. I haven't tested it extensively, so double check if the results look decent 😉 The program calls changed slightly, see here: github.com/warpem/miss-...

I will be hiring at all levels so if you are interested and want to learn more, please get in touch! 📻 ❄️ 🔬 🦠 🧬 #cilia #cryoet #proteomics #exm #protistsonsky

Happy to finally share that I will be starting my independent group at the @fmiscience.bsky.social in Feb 2027 in a vibrant new environment. We will use integrative in situ structural cell biology methods to investigate ciliary structure and dynamics in several model (and non-model 👀) systems!!

Pyrenoid tubules are essential for supercharging CO₂ fixation, but they're hard to resolve by microscopy. So, we physically expanded cells to see where different proteins go (3 examples below). The central tubule region appears specialized for CO₂ delivery! www.biorxiv.org/content/10.6...

New preprint! In collaboration with @xujwet.bsky.social, @pedrobeltrao.bsky.social, and Akhmanova and Beekman groups, we determined the structure of the transition zone microtubule doublet in mammalian motile cilia, providing potential insights into the regulation of IFT: tinyurl.com/fssnevuc

Structure of the mammalian ciliary transition zone microtubule doublet

The ciliary transition zone gates bidirectional protein trafficking to maintain the specialized ciliary proteome using microtubule doublets as a scaffold. While ciliary axonemal doublets are well-char...

tinyurl.com

Here's an excerpt from the most recent video on how Shannon studied the entropy of English, animated by Mitchell Zemil

We have just published a detailed, simple, and effective protocol for the electroporation of the microalga Chlamydomonas. Check it out! bio-protocol.org/en/bpdetail?...