The self-driving multiscale microscope from @daetwylerstephan.bsky.social is out. It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution. We used it to study cancer-immune cell interactions. www.nature.com/articles/s41...

Imaging of cellular dynamics from a whole organism to subcellular scale with self-driving, multiscale microscopy

Nature Methods - A self-driving multiresolution light-sheet microscope enables the simultaneous observation and quantification of cellular and subcellular dynamics in the context of intact and...

nature.com

Goodbye Mr Snouty. I had a lot of fun working with you. It has been a blast. Hopefully the next scientist/engineer who works with you will enjoy the process too.🥹

Science bio-imaging question: What platform are people liking for confocal / super-resolution imaging these days? Leica? Zeiss? Olympus? Nikon? Other?

Hello science friends on bluesky! After many years of doing molecular biophysics and optics/microscopy, I just want to share that I'm leaving science and academia next month. I don't have concrete plans for my next step yet so I might just take a break for a while but we shall see how things go.

Also in today's preprint trawling: imaging at depth is difficult, so let's just... squish cells? arxiv.org/abs/2411.12656

search.app?link=https%3...

Probe set selection for targeted spatial transcriptomics - Nature Methods

Spapros is a probe set selection pipeline for targeted spatial transcriptomics that optimizes for both transcriptional and within-cell type variation.

search.app

Does anyone know any good publications/databases to find non-coding variant loci for neurodevelopmental disorders? (anything, but particularly disorders of brain size if possible)

The pSABER paper is now online! pSABER is a new method for amplifying in situ hybridization signals This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social doi.org/10.1038/s415... 🧵 1/12

Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER - Nature Methods

pSABER combines the power of signal amplification by exchange reaction (SABER) with the deposition of fluorescent or colorimetric substrates by horseradish peroxidase to enable enhanced signals for in...

doi.org

Caring for your friends in science means lending them your neutral density filter wheel to see if it will help to alleviate their microscope problems

please don't do this. but i do have objectives that are broken and beyond repair. and i also do have immersion oil in the lab. and i also do have an optical table. hmmm

DrLachie @drlachie.bsky.social · 2y ago

So.....don't do this?

Hello everyone! So many of you have followed me over the past several days. I'm guessing most are interested in my science research work (i.e., fluorescence microscopy, image analysis, spatial transcriptomics). So I decided to wind down my bird app account and slowly post a little more on Bluesky.

Made a starter pack for microscopy and image analysis - please (self-)nominate additions! go.bsky.app/Nwa91pC

I had the opportunity to conduct a hands-on workshop on basic optical alignment today! Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!

The 21st Annual Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications hosted by the CRL Molecular Imaging Center at UC Berkeley on 23-25 Jan 2024. www.eventbrite.com/e/21st-annua...

21st Annual Advanced Imaging Methods Workshop

The Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications.

eventbrite.com

I think these are my favorite forever stamps ever!🖤🤍♥️💚🩵🧡

AI powered microscope to spot cancer? Built by Google and DOD? www.cnbc.com/2023/09/18/g...

Google and the Department of Defense are building an AI-powered microscope to help doctors spot canc...

The AI-powered tool is called an Augmented Reality Microscope, or ARM, and Google and the Department of Defense have been quietly working on it for years.

cnbc.com

Singapore's a blue zone city!?!?! I guess my life expectancy is ~81 finance.yahoo.com/news/longevi...

The longevity secrets of Singapore, the 6th blue zone city where people are living the longest, happ...

The five health and happiness secrets of Singapore, "blue zone 2.0—the next frontier of aging."

finance.yahoo.com

We have started to work on 3D spatial transcriptomics of the whole larval zebrafish brain and I'm excited to share an image that we recently acquired in the lab! Every bright spot or blob represents the RNA molecules that we've labelled and imaged! The image below is 1750*1050 microns.

"By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain." https://www.nature.com/articles/d41586-023-02559-9

The quest to map the mouse brain

By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain. By combining single-cell ...

nature.com

Dr Waters is searching for an advanced microscopy fellow! https://microfellows.hms.harvard.edu/apply/

Yes! I feel like I'm working on a technology of the future now. "we foresee that spatial omics will likely evolve toward three-dimensional spatial omics (operating on whole organs or even organisms)" https://www.science.org/doi/10.1126/science.abq4964

I'm still amazed that my Pixel 7 can see stars with the astrophotography mode amidst all that light pollution in Singapore.

It was a sunny day at #BiopolisSingapore

We finally got a micrometer screw gauge (with a ratchet) to measure the thicknesses of our #1.5 glass coverslips to see how close they are to the specified 0.170 mm thickness with micron precision! The first two were 0.183 and 0.212 mm thick.🤷🏻‍♂️

Soviet scientist Flyorov had found that American/British scientists, who wrote on nuclear developments before mid-1940, had stopped publishing. https://www.washingtonpost.com/archive/politics/1979/07/27/soviets-had-chance-to-develop-first-a-bomb-historian-says/49b94c40-d210-459b-89a0-98c9d2109b8f/

Got a chance to enjoy the hot weather and see some touristy sights in Singapore today!

For newly arrived Bluesky life scientists, I would recommend the Micro-Nano-Bio feed. This still needs optimizing but it pulls in any posts with the following keywords: 🔬 microscopy "super resolution" "single molecule" "localization microscopy" SMLM 🦠 nanotechnology

Made a feed tracking "bioimaging", "microscopy", and "bioimage analysis" (I hope to make something fancier later that can take more than 3 terms, but "perfect" is the enemy of "done" and I'm super grateful track.goodfeeds.co makes it easy to make one). Please share if you like those terms!