Mayank Sharma
@mayankdu.bsky.social
Jr. Group Leader/Lecturer @ETH Zürich, Plant Cell Biology #chloroplasts #starchbiosynthesis #membraneremodeling #mitochondria (tweets=my opinion)
New PEC story 🌱 led by PhD cand Susi with amazing collabs Grimm, Hause & Robatzek labs! We patch-clamped isolated plastids to show that PECs mediate rapid cation flux across the IE. PECs relay Ca²⁺ signals into plastids & link Ca²⁺ to JA-mediated defense. #plantscience www.pnas.org/doi/10.1073/...
Congratulations to Susanne, Henning @kunzlab.bsky.social and the team on this exciting new publication in @pnas.org 🎉🌱 Insightful work connecting plastid ion channels, Ca+ signalling, hormone biology, and plant immunity! 👏 doi.org/10.1073/pnas...
PLASTID ENVELOPE ION CHANNELS (PEC1/2) link Ca2+ and jasmonic acid signaling in plant cells | PNAS
Plants constantly encounter adverse environmental interactions. One organelle is particularly specialized in stress signaling and phytohormone synt...
doi.org
Dry seeds are not waiting to build mitochondria—they come pre-packaged with cristae and full respiratory complexes. ⚡️🌱🌱🌱 Check out the updated growth-and-division model in @currentbiology.bsky.social doi.org/10.1016/j.cu...
Our single-cell atlas paper made the cover 🎉 image by @lbmountain.bsky.social, showing the mature Brachy epidermis in all its beauty. #GoBrachy: yellow is lignin, and blue is cell wall autofluorescence.
Michael Raissig@michaelraissig.bsky.social · 3mo ago🌾 Grass leaves are the photosynthetic powerhouses of human civilisation. @lbmountain.bsky.social's transcriptomic atlas of nearly 70,000 cells from shoot apex to mature leaf follows the genetic programs that form a grass leaf 🧬 paper doi.org/10.1093/plce... expression browser shiny.ips.unibe.ch
Very proud to see this paper out. This is an incredible collaboration with the labs of Wes Sundquist and Adam Frost. @uofubiochem.bsky.social @kingslsm.bsky.social www.nature.com/articles/s41...
ESCRT-III assembles around mis-segregated DNA to protect genome stability - Nature Structural & Molecular Biology
The authors show that persistent ultrafine DNA bridges become coated by membranes and the ESCRT-III proteins CHMP1B and IST1, which protect the genome within from damage.
nature.com
1st insights into protein synthesis accuracy of plant organelles: www.pnas.org/doi/full/10.... Thx to terrific colabs @ifink-lab.bsky.social @organelledchaos.bsky.social & many others from @pullman.wsu.edu @hhu.de @uni-muenster.de @unihalle.bsky.social funds by @dfg.de #GoPMF #plantscience #biology
Hi #plantscience peeps, please check out our revised manuscript version under: www.biorxiv.org/content/10.6...
biorxiv.org
KunzLab@LMU@kunzlab.bsky.social · 10mo agoPlz check out our preprint in which we investigate the role of KEA1 in #chlamy, a monoplastid algae. KO mutant vs WT comparison across the green linage reveals conserved but also novel insights in part linked to the mono plastid bottleneck. Thx to many terrific collabs 👇 doi.org/10.64898/202...
New preprint 🚀 ❄️ We developed Autogrid-compatible HPF planchettes that enable direct freezing of clipped EM grids and streamline integration with cryo-FIB workflows ✔️ improved grid stability ✔️ reduced handling ✔️ robust vitrification (🦠 🧫 🧠) A step toward routine HPF for complex samples #teamtomo
New paper out from the Synoxys lab! We asked an important question: what if plants sensed oxygen like animals do? what if we swapped the O2 sensing across? We built the system to find out. 🧵 www.nature.com/articles/s41... #plantscience @syno2xis.bsky.social @beagiuntoli.bsky.social @biology.ox.ac.uk
a poster for indiana jones and the last crusade with a man in a hat
ALT: a poster for indiana jones and the last crusade with a man in a hat
media.tenor.com
A Recent one on plastid intron splicing from B. Pfister. rdcu.be/e9vKl
Maturase K forms a plastidial splicing complex with a neofunctionalized branching enzyme
Nature Communications - Group II intron splicing in plant chloroplasts likely requires Maturase K, a relative of bacterial maturases. Here, the authors demonstrate that, unlike its bacterial...
rdcu.be
At long last, the Chlamy chloroplast ribosome hat 🎩 (now renamed the arm 💪) is here to say hello👋! We first spotted this extended structure in green algae a decade ago, and @florentwaltz.bsky.social did beautiful work bringing it into focus! #CryoEM ❄️ #TeamTomo 🔬 #PlantScience 🌾 #ProtistsOnSky 🧪
Florent Waltz@florentwaltz.bsky.social · 7mo agoLong in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵
Plz read our study by @lmumuenchen.bsky.social PhD cand. Lorenz. He describes chloroplast ionomes for several 🌱species & shows how plastid iron can be massively increased. Terrific help by peers from @ruhr-uni-bochum.de, @mizzouid.bsky.social and @uni-saarland.de academic.oup.com/plcell/artic...
The chloroplast ionome shines light on the dynamics of organellar iron homeostasis
Loss of chloroplast iron (Fe) ferritin storage leads to Fe re-shuttling into the vacuole and reduced leaf tissue Fe content.
academic.oup.com
Plz check out our preprint in which we investigate the role of KEA1 in #chlamy, a monoplastid algae. KO mutant vs WT comparison across the green linage reveals conserved but also novel insights in part linked to the mono plastid bottleneck. Thx to many terrific collabs 👇 doi.org/10.64898/202...
Now online in its final version. Plz surf to doi.org/10.1111/tpj....
Catch & Release—rapid cost‐effective protein purification from plants using a DIY GFP‐Trap‐protease approach
‘Catch & Release’ represents a cost-effective, rapid, and reliable one-step protein purification workflow that accelerates plant research. It offers a functionality-confirmed and versatile binary vec...
doi.org
KunzLab@LMU@kunzlab.bsky.social · last yr.Preprint from @dfg.de #GoPMF. All 🌱 scientists love to get their hands on pure protein, quickly produced in 🪴. However, purification can take too long, involve many steps or requires pricey reagents. @lmumuenchen.bsky.social , we established Catch & Release. www.biorxiv.org/content/10.1...
1/ Preprint alert: 🌾 The developing leaf of the wild grass Brachypodium distachyon at single-cell resolution 👉 doi.org/10.1101/2025... A 70k-cell single-cell RNA-seq atlas of the developing grass leaf—from the shoot meristem to mature leaf tissues. @cerealcell.bsky.social @lbmountain.bsky.social
Hi all @plantsciencedbg.bsky.social members and other 🌽community folks, #MBP2026 🌱 is no open for registration. Please 🏄♀️ to: www.pflanzen-molekularbiologie.de/en/conferenc...
Conference
Conference "Molecular Biology of Plants", annual meeting of the Section Plant Physiology and Molecular Biology in the DBG
pflanzen-molekularbiologie.de
Hey #TeamTomo …would you like to try some 🍦ICECREAM!? Our @unibas.ch colleagues over at the @ivandokmanic.bsky.social lab have developed a stunningly effective tool to de-noise and de-wedge your tomograms. The contrast and details will give you brain freeze! 🍨❄️🤯 Please try, we need feedback 🧪🧶🧬🔬
Ricardo D. Righetto@lifeonthewedge.bsky.social · 11mo agoOur colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Concluded the teaching for this semester. 🧑🏫 The greatest feeling is to transfer the knowledge. 💡
What else I am offering • My services for transforming PhD theses into Thesis Glossies with Thesis & Zo • And not to forget my blogs about recent plant science articles under Plant & Zo
Mayank Sharma@mayankdu.bsky.social · 11mo agoHot off the press, first PI paper 🎉. Reporting a novel protein (VIA1) involved in thylakoid membrane biogenesis. VIA1 (similar to ESCRT-II) and bind to chloroplast ESCRT-III (VIPP1). Showing a direct role of VIPP1 in thylakoid biogenesis. @cp-cellreports.bsky.social tinyurl.com/msrepdna
So, we all know that VIPP1 (the Very Important Protein in Plants) is the "photosynthetic ESCRT-III", required for the biogenesis and maintenance of light-harvesting thylakoids. But what about ESCRT-II, you say? ...Meet VIA1! Congrats @mayankdu.bsky.social on your lab's first paper! #PlantScience 🧪 🌾
Mayank Sharma@mayankdu.bsky.social · 11mo agoHot off the press, first PI paper 🎉. Reporting a novel protein (VIA1) involved in thylakoid membrane biogenesis. VIA1 (similar to ESCRT-II) and bind to chloroplast ESCRT-III (VIPP1). Showing a direct role of VIPP1 in thylakoid biogenesis. @cp-cellreports.bsky.social tinyurl.com/msrepdna
Hot off the press, first PI paper 🎉. Reporting a novel protein (VIA1) involved in thylakoid membrane biogenesis. VIA1 (similar to ESCRT-II) and bind to chloroplast ESCRT-III (VIPP1). Showing a direct role of VIPP1 in thylakoid biogenesis. @cp-cellreports.bsky.social tinyurl.com/msrepdna
New work from the Synoxys lab! About how does a plant’s habitat, whether growing in water, drylands, or typical soils, shape the evolution of its oxygen-sensing machinery. Indulge! #plantscience #ISPLORE2025JP
Ximena Chirinos Herrera@ximenachirinos.bsky.social · 12mo agoThrilled to make my first post here 🌱 Right after presenting at #ISPLORE2025JP, where I shared our work on how anatomy & habitat shaped the oxygen-sensing machinery of angiosperms. Please check out our new preprint www.biorxiv.org/content/10.1...
Honoured & excited that ‘Breathing Underground’ has been funded by the ERC! This will let me explore one of the most fundamental questions of plant life: how roots breathe underground? @erc.europa.eu
It is not often that you see shiny flowers but these water-buttercup, Ranunculus lingua, are definitely shiny. They thank this to a unique and rare combination of traits. 🧵1/3
Our new review with Benoit Menand and Jose Crespo on the emerging connections between TOR, chloroplasts and photosynthesis 😎 in plants 🌿and algae 🦠 #PlantScience url: academic.oup.com/jxb/article-...
Starch Enthusiasts can register for Starch Round Table 2025 by 3rd October: Minneapolis USA commerce.cashnet.com/KSUGSI
Transact Campus
commerce.cashnet.com
Webinar : Optimizing Immunoprecipitation of FP-Tagged Proteins in Plant Cell Extracts 📅 Date: August 7th, 2025 🕒 Time: 9AM CDT / 3PM BST / 4PM CEST 🔗 Register here: ptglab.zoom.us/webinar/regi... #PlantBiology #ProteinBiochemistry #Immunoprecipitation #GFPTrap #MolecularBiology
