This story is now published in @natplants.nature.com ! 🎉🎩 Check out the final article here: nature.com/articles/s41477-026-02361-1 Very similar to the original bioRxiv preprint, with the addition of some cool polysome analysis (in the supplementals)! Thanks and congrats to everyone involved! 🙏

Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome - Nature Plants

Using in situ cryo-electron tomography, researchers found a unique ‘arm’ domain on the small subunit of Chlamydomonas chlororibosomes. This discovery suggests that chlororibosome structure is more evo...

nature.com

Florent Waltz@florentwaltz.bsky.social · 6mo ago

Long in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵

New preprint from the lab! Led by graduate student @lainahall.bsky.social we show a new method to thin cells for cryo-EM imaging with minimal damage. www.biorxiv.org/content/10.6...

Optimized cryo-FIB milling strategy to generate thin, minimally damaged biological lamellae

Focused ion beam (FIB)-milling has been adapted to thin frozen cells for visualization of macromolecular structures in situ with cryogenic electron microscopy. However, only a few large and abundant c...

biorxiv.org

Some bacteria do not live as individuals. They build multicellular filaments and connect neighboring cells through tiny cell-cell junctions. Cyanobacteria are among the most beautiful examples 😍 In our new paper, we reveal first molecuar insights on the cyanobacterial septal junction architecture 🧵

Super excited to launch this search for a new endowed professor at the @biozentrum.unibas.ch! Join us to build a research cluster in Climate Biophysics! 🌍🌊🌾🔬🧪 This is a unique opportunity to create a community in Basel🇨🇭working together on this timely topic! ⏳🌡️ Questions? Please reach out! 👩‍🔬👨‍🔬

Biozentrum, University of Basel@biozentrum.unibas.ch · last mo.

New Assistant (tenure-track) or Associate Professor position in Climate #Biophysics! Join our international research community to explore fundamental molecular mechanisms underlying climate-relevant biological processes. Apply by 7 Sept 2026! www.biozentrum.unibas.ch/de/open-posi...

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

Philippe Van der Stappen@phaips.vd.st · last yr.

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

New preprint! In collaboration with @xujwet.bsky.social, @pedrobeltrao.bsky.social, and Akhmanova and Beekman groups, we determined the structure of the transition zone microtubule doublet in mammalian motile cilia, providing potential insights into the regulation of IFT: tinyurl.com/fssnevuc

Structure of the mammalian ciliary transition zone microtubule doublet

The ciliary transition zone gates bidirectional protein trafficking to maintain the specialized ciliary proteome using microtubule doublets as a scaffold. While ciliary axonemal doublets are well-char...

tinyurl.com

In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn

🛠️💉It's time for engineering contractile injection systems! @chipericson.bsky.social & @davidschaumann.bsky.social introduce programmable CIS - PROCIS, combining tail length control, non-native cargo loading, and cell retargeting all in one system! www.biorxiv.org/content/10.6... 🧵 1/5

We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇

Some phages are named after gods or rivers, this one was named by journalist Thilo Mischke @thilomischke.bsky.social after his grandma ("Oma Karin") while filming in our lab. It's a cool phage that should be temperate but became virulent due to a transposon at its lysogeny control! ⛓️‍💥💪🏼

The really striking part: all three Dip proteins oscillate pole-to-pole, and DipB and DipC also build ring-like structures at midcell. This is reminiscent of the bacterial Min system, which uses oscillation to keep FtsZ centered — but Dip is its own thing (more on that below).

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Really important and relevant study in TB from @danbarberphd.bsky.social and colleagues. We need to re-think many assumptions. Nitric oxide production in mice has been a confounding factor. #tuberculosis

Daniel L. Barber, PhD@danbarberphd.bsky.social · 3mo ago

So we blocked IFNγ in Mtb infected macaques and….the animals were fine. Turns out mice overestimate the importance of IFNγ in TB because IFNγ drives iNOS in mice but not macaques or humans. This means we don’t actually know how T cells suppress Mtb. rdcu.be/ff9GB #tuberculosis #immunology

I am happy to share a review I recently wrote on the design of peptide binders. It gives an overview of experimentally validated tools and discusses the challenges of why peptide design is more difficult than the design of classical protein binders. www.chimia.ch/chimia/artic...

If you wanna try our new Autogrid High-Pressure Freezing Planchettes ❄️ - you can use the provided drawings & CAD files to produce them in-house 🛠️ - you can now also contact www.wohlwend-hpf.ch to order yours with a shiny gold-coating ✨ The preprint for instructions ➡️ www.biorxiv.org/content/10.6...

📜The final version of our paper on eCIS firing is out: www.nature.com/articles/s41... Congrats to @xujwet.bsky.social @chipericson.bsky.social #cryoEM #CIS

Pilhofer Lab 🦠❄️🔬@pilhoferlab.bsky.social · 10mo ago

🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social&@chipericson.bsky.social trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬 www.biorxiv.org/content/10.1...

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

VERY nice video, and a nice invention! Thanks again Miriam Weber who presented this at CryoNET 2025 in Umeå.

Gregor Weiss@weiss-lab.bsky.social · 4mo ago

New preprint 🚀 ❄️ We developed Autogrid-compatible HPF planchettes that enable direct freezing of clipped EM grids and streamline integration with cryo-FIB workflows ✔️ improved grid stability ✔️ reduced handling ✔️ robust vitrification (🦠 🧫 🧠) A step toward routine HPF for complex samples #teamtomo

New preprint 🚀 ❄️ We developed Autogrid-compatible HPF planchettes that enable direct freezing of clipped EM grids and streamline integration with cryo-FIB workflows ✔️ improved grid stability ✔️ reduced handling ✔️ robust vitrification (🦠 🧫 🧠) A step toward routine HPF for complex samples #teamtomo

Out now in @science.org Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape | Science www.science.org/doi/10.1126/...

Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape

Bacteria, like eukaryotes, use conserved cytoskeletal systems for intracellular organization. The plasmid-encoded ParMRC system forms actin-like filaments that segregate low–copy number plasmids. In m...

science.org

Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape | Science www.science.org/eprint/JCFZX...

Super cool story on one of the most beautiful bacteria out there: Cyanobacteria 😍 congrats to @huepfkiesel.bsky.social, @nartimsoole.bsky.social & co-authors!

Martin Loose@nartimsoole.bsky.social · 4mo ago

Now out in its final form! www.science.org/doi/10.1126/...

Now out in its final form! www.science.org/doi/10.1126/...

Martin Loose@nartimsoole.bsky.social · last yr.

One of the most exciting discoveries from our lab so far is now online as a preprint! Read a story on how the ParMR and Min systems started to collaborate to create the CorMR cytoskeleton, which now controls cell shape in multicellular cyanobacteria: www.biorxiv.org/content/10.1...

We're excited to announce the beta-release of #RELION-5.1! 🥳 This release has important updates on our #amyloid processing pipeline, as described in this preprint: doi.org/10.64898/202... There are also some tweaks for #teamtomo. #OpenSoftwareAcceleratesScience

What a pleasure to give my first talk with a lab coat at Institute of Medical Microbiology, University of Zurich (UZH). Feeling like old school professor. 😅😅 Thank you @weiss-lab.bsky.social for the invitation, and the amazing time with an amazing team. 🥳 #UTI #AMR #organoids #UTISky

What if quorum sensing doesn’t promote biofilms - but restrains it? 🤔 New work from @harisantypas.bsky.social shows that the Fsr QS system in Enterococcus faecalis acts as a brake on biofilm formation and pathogenesis during infective endocarditis (IE). www.nature.com/articles/s41...

Loss of Fsr quorum sensing promotes biofilm formation and worsens outcomes in enterococcal infective endocarditis - Nature Communications

Infective endocarditis (IE) is an understudied biofilm-associated infection. Here, analyses of preclinical and clinical data reveal that the Fsr quorum sensing (QS) system of Enterococcus faecalis is ...

nature.com

Our latest preprint: Together with the team of Jan Löwe, @danieltamarit.bsky.social and many others we discovered and characterized several Asgard tubulin genes and propose that microtubule architecture and dynamics evolved in Asgard archaea prior to eukaryogenesis www.biorxiv.org/content/10.6...

Contractile Injection Systems - Hot Springs - cryoET: Read the final version of our paper here: academic.oup.com/ismej/advanc... #microsky #teamtomo

Thermophilic bacteria employ a contractile injection system in hot spring microbial mats

Abstract. Bacterial contractile injection systems (CISs) are multiprotein complexes that facilitate the bacterial response to environmental factors or inte

academic.oup.com

Excited to share this detailed biophysical study of #PhaseSeparation in the Pyrenoid, an algal microcompartment that fixes a third of the Earth's #CO2 🌏. We combine methods across scales, in vitro 🧪 and in vivo 🦠 Thanks for the very fun collaboration! 🤗 #PlantScience 🌾 #TeamTomo 🔬 #LivingPhysics 🧬🧶

James Barrett@james-r-barrett.bsky.social · 6mo ago

How does molecular valency shape condensate assembly and function? We used the CO2-fixing organelle in algae—the pyrenoid—to find out… 🧵 Preprint here!: www.biorxiv.org/content/10.6... @cellarchlab.com @phaips.vd.st @biologyatyork.bsky.social #Rubisco #PhaseSeparation #Condensates #Pyrenoid

The origin of eukaryotic cells continues to be one of the greatest mysteries in biology. EMBL Grenoble is excited to welcome Florian Wollweber, who will use cutting-edge imaging techniques to study Asgard archaea — microbes closely related to our earliest ancestors. 🦠🔬 www.embl.org/news/people-...