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Dmitry Sutormin

@monochamussutor.bsky.social

Scientist, molecular biologist and bioinformatician with a focus in microbiology

3 Posts28 Follower39 Folgt
Dmitry Sutormin@monochamussutor.bsky.social · 2d ago

🚀 Thrilled to share our first metaSPLiT preprint from @annabiosys.bsky.social's lab in collaboration with @gibbological.bsky.social! Explore hidden bacterial phenotypic heterogeneity in the human gut microbiome and discover the concept of "physiological guilds": www.biorxiv.org/content/10.6...

Phenotypic heterogeneity in the human gut microbiome revealed by subspecies-resolution single-cell transcriptomics

Most of our knowledge about bacterial functional roles in microbiomes comes from bulk measurements. Yet microbial communities are complex ecosystems in which functionally distinct bacterial subpopulat...

biorxiv.org

Anna Kuchina@annabiosys.bsky.social · 5mo ago

Paper out: Profiling of lytic phage infection in individual B. fragilis cells with bacterial scRNA-seq. Congratulations Anika Gupta, Norma Morella, @monochamussutor.bsky.social Dmitry Sutormin et al, in collaboration with Georg Seelig (UW) and Neel Dey (Fred Hutch). www.nature.com/articles/s41...

Dynamics of phage-host interactions in Bacteroides fragilis resolved by single-cell transcriptomics - Nature Communications

Phage-bacteria interactions are typically studied in bulk culture, which obscures cell-cell differences. Here the authors study phage-bacteria interactions using single-cell transcriptomics, identifyi...

nature.com

Anna Kuchina@annabiosys.bsky.social · 8mo ago

Pooled CRISPR screens with transcriptomics readout arrived for bacteria. We introduce mapSPLiT, an approach to profiling transcriptional responses to CRISPR perturbations at scale. Collaborative work with Carothers, Zalatan, and Seelig groups at UW&@isbscience.org www.biorxiv.org/content/10.6... 1/4

Pooled single-cell CRISPRa/i screens for functional genomics in bacteria at scale

Pooled CRISPR screens using single-cell RNA sequencing (scRNA-seq) have emerged as powerful tools to uncover gene function, map regulatory networks, and identify genetic interactions. However, the inh...

biorxiv.org

Anastasiia Rusanova@anastasiiar.bsky.social · 10mo ago

It’s finally out! Our paper on the bacterial symbionts of three White Sea sponges is here. A story that began in 2016 has reached its last chapter… or has it? We hope that by the time you finish our hefty tome — maybe around 2034 — we’ll release the sequel. journals.asm.org/doi/10.1128/...

Taxonomically different symbiotic communities of sympatric Arctic sponge species show functional similarity with specialization at species level | mSystems

Sponges are regarded among the earliest multicellular organisms and the most ancient examples of animal-bacterial symbiosis. The study of host-microbe interactions in sponges has advanced rapidly due ...

journals.asm.org

@artemisaev.bsky.social · last yr.

After many years in the making, this story is finally published! In the updated manuscript on BREX DNA recognition, we reveal that modifying the system's specificity can surprisingly enhance anti-phage defense. www.nature.com/articles/s41...

Molecular basis of foreign DNA recognition by BREX anti-phage immunity system - Nature Communications

BrxX methylase in complex with SAM cofactor mediates foreign DNA recognition by the BREX system. BrxX can be engineered to modify BREX specificity and enhance defense. BREX defense and methylatio...

nature.com

Anastasiia Rusanova@anastasiiar.bsky.social · last yr.

Check it out! Three Arctic sponge species, six years of observation, and seven symbiotic bacteria—six of which belong to novel candidate genera. An odyssey that began in 2016 is now complete! www.biorxiv.org/content/10.1... #Sponges #Microbiome #Symbiosis #BacteriaSymbionts #Arctic

Taxonomically different symbiotic communities of sympatric Arctic sponge species show functional similarity with specialization at species level

Marine sponges harbor diverse communities of associated organisms, including eukaryotes, viruses, and bacteria. Sponge associated microbiomes contribute to the health of the host organisms by defendin...

biorxiv.org

Anna Kuchina@annabiosys.bsky.social · 2y ago

How can single-cell transcriptomics profile phage infection in individual microbial cells? Check out our preprint with Anika Gupta, Norma Morella, Dmitry Sutormin & other authors, in collaboration with Georg Seelig (UW) and Neel Dey (Fred Hutch). www.biorxiv.org/content/10.1...

Combinatorial phenotypic landscape enables bacterial resistance to phage infection

Success of phage therapies is limited by bacterial defenses against phages. While a large variety of anti-phage defense mechanisms has been characterized, how expression of these systems is distribute...

biorxiv.org