Visualization of stepwise derepression of TFIIH in global genome nucleotide excision repair pubmed.ncbi.nlm.nih.gov/42490432/ #cryoEM

I am so glad to share that our paper is finally out!!!

Basil Greber@bjgreber.bsky.social · 2mo ago

Happy that our most recent paper on nucleotide excision repair mechanisms has now been published in Science Advances. This project was led by @natalia-mg.bsky.social and @callumhaste.bsky.social and focused on visualising early steps of the NER process by #cryoEM. www.science.org/doi/10.1126/...

Happy that our most recent paper on nucleotide excision repair mechanisms has now been published in Science Advances. This project was led by @natalia-mg.bsky.social and @callumhaste.bsky.social and focused on visualising early steps of the NER process by #cryoEM. www.science.org/doi/10.1126/...

Visualization of stepwise derepression of TFIIH in global genome nucleotide excision repair

Cryogenic electron microscopy captures nucleotide excision repair complexes assembling on damaged DNA templates.

science.org

📢 New research details how an important cellular enzyme is controlled, uncovering new insights into the fundamental biological processes controlling gene expression.

Last week, I had the pleasure of attending the Responses to DNA Damage meeting in Egmond aan Zee. It was truly inspiring and exciting to see the incredible science being carried out in DNA repair, and even more exciting to present my work in one of the plenary sessions.

Sub-2 Å cryo-EM structures of transcribing RNA polymerase II reveal critical roles of water molecules in catalysis #CryoEM www.cell.com/molecular-ce...

Sub-2 Å cryo-EM structures of transcribing RNA polymerase II reveal critical roles of water molecules in catalysis

The roles of water molecules in transcription have long been overlooked due to resolution limitations. Li et al. resolve high-resolution cryo-EM structures of RNA polymerase II and visualize previousl...

cell.com

Have a look at our latest results on #CDK regulation, just posted as a pre-print! This study was spearheaded by PhD student Amy McGeoch. Amy has determined the #cryoEM structure of the CDK11-cyclin L-SAP30B complex, an important regulator of #spliceosome activation. www.biorxiv.org/content/10.6...

The final version of our article on XPF-ERCC1-SLX4 complexes has now been published (the link below still works). I am happy to report that the rendering of our DNA gels has now been corrected. 😉

Basil Greber@bjgreber.bsky.social · 9mo ago

Our latest paper on the molecular mechanisms of XPF-ERCC1 recruitment to SLX4-dependent DNA repair pathways has been published! Congratulations to first author Junjie Feng, and a heartfelt thank you to all our collaborators for their contributions to this study! www.nature.com/articles/s41...

An early Christmas present! The ICR’s new Hydra Bio FIB-SEM has been delivered, which will sit at the heart of a consortium between the @icr.ac.uk, Imperial, QMUL and KCL. Currently being stored at Imperial, it’ll be installed in a brand new lab at the ICR next year. Exciting things coming in 2026!🔬

Our latest paper on the molecular mechanisms of XPF-ERCC1 recruitment to SLX4-dependent DNA repair pathways has been published! Congratulations to first author Junjie Feng, and a heartfelt thank you to all our collaborators for their contributions to this study! www.nature.com/articles/s41...

Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control. www.science.org/doi/10.1126/...

Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase

Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...

science.org

New paper out now in Science! Check it out! www.science.org/doi/10.1126/...

Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase

Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...

science.org

Great effort from everyone!

Basil Greber@bjgreber.bsky.social · last yr.

Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone! www.nature.com/articles/s41...

Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone! www.nature.com/articles/s41...

High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants - Nature Communications

Many protein–protein interactions depend on Short Linear Motifs (SLiMs). In this study, the authors use large-scale binding assays, deep mutational scanning, and structural analysis to map SLiMs recog...

nature.com

Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out: www.sciencedirect.com/science/arti...

Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV

Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report s…

sciencedirect.com

🎉👏 ICR's Dr. Natàlia de Martín Garrido @natalia-mg.bsky.social, a postdoc fellow in Dr. Basil Greber's lab @bjgreber.bsky.social was awarded the 'Short Talk Award' for her research on DNA repair at the “Machines acting on DNA and RNA, a molecular mechanistic perspective” conference held in Madrid.

Very grateful for receiving a Short Talk Award at #CFM_MolMech Thanks to the organisers for putting together such an amazing conference! @oscarllorca.bsky.social @rfleiro.bsky.social @nogaleslab.bsky.social @cniostopcancer.bsky.social

Basil Greber@bjgreber.bsky.social · last yr.

Delighted to hear that post-doc @natalia-mg.bsky.social has received the Short Talk Award for her #cryoEM studies of DNA repair at the conference on "Machines Acting on DNA and RNA, a molecular mechanistic perspective" at CNIO Madrid. Congratulations, Natàlia! Photo courtesy of @amunts.bsky.social.

Delighted to hear that post-doc @natalia-mg.bsky.social has received the Short Talk Award for her #cryoEM studies of DNA repair at the conference on "Machines Acting on DNA and RNA, a molecular mechanistic perspective" at CNIO Madrid. Congratulations, Natàlia! Photo courtesy of @amunts.bsky.social.

For our latest paper, we worked with SYROS Pharmaceuticals, the groups of Dylan Taatjes and Robin Dowell at CU Boulder, and @abhaykot.bsky.social. Junjie Feng in my lab determined the #cryoEM structure of the CDK7 inhibitor SY5609 bound to its target... www.science.org/doi/10.1126/...

TFIIH kinase CDK7 drives cell proliferation through a common core transcription factor network

CDK7 kinase activity is found to control RNAPII transcription through a cohort of TFs that drive cell cycle and proliferation.

science.org

#InnovacióUB | 🔬 El president @salvadorilla.bsky.social i la consellera de Recerca i Universitats, Núria Montserrat, inauguren el nou microscopi electrònic d’última generació ubicat als espais que els @ccit.ub.edu tenen al @www.pcb.ub.edu. L’aparell obre la porta a estudis innovadors.

Illa demana «ambició i lideratge de la recerca catalana» en la inauguració del nou microscopi de la UB

web.ub.edu

New pre-print! To support a CDK-cyclin docking motif discovery and characterisation project led by Norman Davey and colleagues, @natalia-mg.bsky.social in my lab determined the #cryoEM structures of three peptide-bound CDK2-cyclin A complexes. Check it out! www.biorxiv.org/content/10.1...

High-throughput discovery and deep characterization of cyclin-CDK docking motifs

Cyclin-CDKs are master regulators of cell division. In addition to directly activating the CDK, the cyclin subunit regulates CDK specificity by binding short peptide “docking” motifs in CDK substrates...

biorxiv.org

Thrilled to share that I have been selected as one of this year's new EMBO Young Investigators. I look forward to meeting many of the other Young Investigators in the coming years, and I am most grateful to the members of my laboratory at the ICR who made this possible. www.embo.org/press-releas...

Twenty-seven scientists become EMBO Young Investigators – Press releases – EMBO

The group leaders join an international network of nearly 800 life scientists and receive financial support

embo.org