My lab at MSKCC in New York is hiring for two positions. Join us at the frontier of functional genomics, studying fibroblast state transitions, combinatorial genetics, and ECM in disease. Please share with anyone who might be a good fit! (Mustache not required.)

New preprint on technologies to scale up CRISPR screens. We use them to map 665,856 pairwise genetic perturbations and outline a path to comprehensive interaction mapping in human cells. We also introduce an approach for cloning lentiviral libraries with billions of elements.

💫PUBLISHED @natgenet.nature.com 📰Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states. By Kaden M. Southard, @normanlab.bsky.social and colleagues! ⬇️ www.nature.com/articles/s41...

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states - Nature Genetics

CRISPR activation of 1,836 human transcription factors recapitulates fibroblast transcriptional states observed in vivo and identifies regulators that can revert inflammatory states.

nature.com

A mouse organoid platform for modeling cerebral cortex development and cis-regulatory evolution in vitro: Developmental Cell www.cell.com/developmenta...

Our paper is now out in final form at Nature Genetics! For those who missed the preprint, we used large-scale Perturb-seq targeting transcription factors to push primary fibroblasts into diverse transcriptional states, including those observed in cell atlas studies.