@orchardlab.bsky.social

Excited to have this story published @plosbiology.org. We were surprised to find lipids are important for entry of a non-enveloped virus and that TMEM30a is important for enteric infection of murine norovirus!

PLOS Biology@plosbiology.org · last yr.

Norovirus tropism depends partly on entry receptors, but what is the role of cellular #lipids? @orchardlab.bsky.social &co show that TMEM30a-mediated lipid asymmetry is crucial for murine #norovirus infection, influencing membrane fluidity, viral binding & entry @plosbiology.org 🧪 plos.io/4409MtI

Left: Based on electron microscopic (EM) imagery, this three-dimensional (3D) illustration provides a graphical representation of a single norovirus virion, set against a beige background. The different colors represent different regions of the organism’s outer protein shell, or capsid (credit: Unsplash user CDC). Right: BV2 or BV2ΔTMEM30a cells were infected with MNVCR6-HiBiT at an MOI 0.5 in the presence of DMSO, 500 µM GCDCA, or 50 µM C2 ceramide. Sixteen hours post-infection cells were lysed and infection was measured using Nano-Glo HiBiT Lytic Detection System (Promega) post-lysis. Samples are normalized to BV2 cells treated with DMSO.