🚨It's finally out!🥂 | A few years after solving the structure of human cis-PT, we now tackle its dynamics in @NatureComms , revealing that a conserved interfacial loop serves as an allosteric hub, communicating with the active site to regulate catalysis. rdcu.be/eShjl

Structural mechanisms of allosteric regulation in the human cis-prenyltransferase complex

Nature Communications - This study shows how the NgBR subunit controls the activity of human cis-prenyltransferase, a key enzyme for protein glycosylation. Combined experimental and computational...

rdcu.be

Proud of this collaboration - our third paper on cisPT! Loved this one the most: a beautiful mix of high-res, low-res, and computational approaches. Grateful for this long-standing, truly fruitful team effort. Congrats to everyone!

HaitinLab@haitinlab.bsky.social · 8mo ago

🚨It's finally out!🥂 | A few years after solving the structure of human cis-PT, we now tackle its dynamics in @NatureComms , revealing that a conserved interfacial loop serves as an allosteric hub, communicating with the active site to regulate catalysis. rdcu.be/eShjl

Day 1 at the Structural MS Workshop in Biocev! Straight into the lab! Hands-on HDX-MS, cross-linking, and native top-down MS. 🧪 ⚡ ❤️ Thanks to @spidocs.bsky.social, #OPAJK #Photomachines, @brukercorporation.bsky.social @ciisbinstructcz.bsky.social for the support! #Biocev @mbuavcr.bsky.social

🎉Congratulations to the SPIDoc's teams and colleagues for the recent publication "X-ray spectroscopy meets native mass spectrometry: probing gas-phase protein complexes" 👉https://doi.org/10.1039/D5CP00604J 🔥Selected as 2025 Physical Chemistry Chemical Physics HOT article🔥 @rsc.org

Great collaboration (as always)! And very funny story how HDX and native MS complement each other and reveal details about ions, substrates and mutations. We enjoyed this ride!

HaitinLab@haitinlab.bsky.social · last yr.

Excited to share our new paper in @febsj.bsky.social! 🎉 We show how a #MYELOMA-linked GGPPS mutation tweaks hexamerization, substrate binding and product inhibition, revealing an unexpected layer of protein prenylation regulation 🔗 doi.org/10.1111/febs... @peterslab1.bsky.social

Free structural MS hands-on workshop @ BIOCEV (CZ) 📅 Sept 15–19, 2025 For PhDs, postdocs & undergrads (CZ/SK) HDX-MS, native MS, cross-linking, high-res MS, data processing Apply by July 31 → bit.ly/3HNyzIg @spidocs.bsky.social @mbuavcr.bsky.social @ciisbinstructcz.bsky.social

Whenever we get a bioRxiv alert, we'are always curious to see what it brings. This time, the timing couldn’t have been better - a very pleasant surprise! 😄 biorxiv.org/content/10.1... www.biorxiv.org/content/10.1...

This was literally cool work. ❄️ We locked ourselves in the cold room to slow down the HDX and catch even the fastest dynamics. Bonus: It turns out you can manually capture a 2-second exchange reproducibly (next time we set up the quench flow!).

HaitinLab@haitinlab.bsky.social · last yr.

💃🕺Happy to share a team effort to uncover the molecular "dance" behind human cis-prenyltransferase regulation!🔬🧬 @peterslab1.bsky.social @AlbGiacomello #Biochemistry #StructuralBiology @TAUMedFaculty @of_sagol biorxiv.org/content/10.1...

Get ready to take the next step in your career journey! 🚀 Join us for the SPIDoc's Career Day, connect with professionals and discover diverse career pathways. When: May 16, 2025. Where: Biocev, CZ or online. For details, see the flyer peterslab.org/downloads/SP...

Utilization of high-resolution mass spectrometry and data-independent analysis to track the monoclonal antibody spatial stability Expert Rev Proteomics. 2025. Prochazkova V, Rosulek M, Karpisek M, Matousek V, Novak P, Kukacka Z. @peterslab1.bsky.social doi.org/10.1080/1478... #MassSpec #Charlesuni

What a lovely day to close three great PhD chapters from our lab. Fingers crossed, guys!

Recommendations for Automating Hydrogen/Deuterium Exchange Mass Spectrometry Measurements using Data-Independent Acquisition Methods https://www.biorxiv.org/content/10.1101/2025.01.18.633743v1

Great collaboration. This time with some very nice HDX-MS and native MS. Thank you guys!

HaitinLab@haitinlab.bsky.social · 2y ago

🚨New preprint alert! Wonder how a cancer-associated mutation in GGPPS impacts hexameric stoichiometry, substrate affinity, and product inhibition? Our results shed light on a novel regulatory mechanism in protein prenylation bit.ly/3ZR7w47 #MultipleMyeloma

10th Informal Proteomic Meeting #neformal2024 Czech Proteomic section of CSBMB Nov 28 - 29, 2024, Olomouc has just started czproteo.cz/nps_aktualni/

Long overlooked but significant! This is the post-Cys specificity in post-proline cleaving enzymes like AnPEP or neprosin. Good work Zuza and the team!🎉🔥 pubs.acs.org/doi/10.1021/... (illustration IG:kuba_draws_science)