Today in @nature.com we introduce INSTALL, which bypasses mammalian DNA immune sensing to enable non-viral DNA integration with recombinases—a step toward safe, and mutation-agnostic genome editing. 🧬 🧵 (1/13) www.nature.com/articles/s41... @harvardmed.bsky.social @mgbresearch.bsky.social

Immune evasive DNA donors and recombinases license kilobase-scale writing - Nature

INSTALL overcomes fundamental challenges for DNA delivery and integration methods by synergizing immune-stealth nucleic acids with recombinases to enable kilobase-scale integration strategies without ...

nature.com

Just got my first @plasmidsaurus.bsky.social RNASeq data back and am genuinely blown away🤯 Incredibly easy process, and high-quality data back within 7 days, even from here in the UK!

Make your gene knockouts more efficient with multiplexed Cas12a sgRNAs. Our new paper is out now, with tools available from @addgene.bsky.social , www.plasmids.eu and @vdrc-flies.bsky.social. www.nature.com/articles/s41... #CRISPR #geneediting #Drosophila 🧪🧬✂️🔬🪰 Summary 🧵 below.

Excited to share that a part of my PhD work is online now on BioRxiv! www.biorxiv.org/content/10.6... We developed a genome editing approach to target the JAK2 V617F mutation and demonstrate its potential to alleviate MPN hallmarks in primary patient cells. Thread 👇

Check out the latest work from the @davieslab.bsky.social led by Hangpeng!

James Davies@jojdavies.bsky.social · 9mo ago

Our latest paper has just been published in Cell! doi.org/10.1016/j.ce... We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.

We are happy to share our enhancer scramble story, a strategy to create hundreds of stochastic deletions, inversions, and duplications within mammalian gene regulatory regions and associate these new architectures with gene expression levels 🧵 www.biorxiv.org/content/10.1...

Our new preprint is out - please share! We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals. Grab a ☕ and let’s dive in. 🧵🧪 1/n #CRISPR #geneediting

Feels like the migration of science twitter is actually working this time? Exciting!

Some quite interesting observations on length bias of different PCR polymerases, from www.nature.com/articles/s41... Maybe I need to reconsider my unconditional love for Q5...