INSTALLing recombinase-driven genome writing: Engineered single-stranded donors bypass innate immunity to enable large DNA integration

INSTALLing recombinase-driven genome writing: Engineered single-stranded donors bypass innate immunity to enable large DNA integration

In a recent Nature article, Tou et al. introduce INSTALL, a strategy that couples immune-evasive circular single-stranded DNA (cssDNA) technology with recombinase-based genome editing to overcome innate immune barriers and enable the integration of large DNA cargos.

dlvr.it

Read the research highlight from Frank Buchholz in Molecular Cell on INSTALL - a recombinase-driven approach using engineered single-stranded donors that bypass innate immunity. www.sciencedirect.com/science/arti... #Science #Biotech #GeneTherapy #Innovation

INSTALLing recombinase-driven genome writing: Engineered single-stranded donors bypass innate immunity to enable large DNA integration

In a recent Nature article, Tou et al.1 introduce INSTALL, a strategy that couples immune-evasive circular single-stranded DNA (cssDNA) technology wit…

sciencedirect.com

A belated re-post after a brief sci-media hiatus - congratulations to @connorjtou.bsky.social & the team! We sought to overcome a key challenge for inserting large DNAs into the genome: toxicity & innate immune responses. 🧬 🚫 Our paper was published in @nature.com: www.nature.com/articles/s41...

Immune evasive DNA donors and recombinases license kilobase-scale writing - Nature

INSTALL overcomes fundamental challenges for DNA delivery and integration methods by synergizing immune-stealth nucleic acids with recombinases to enable kilobase-scale integration strategies without ...

nature.com

Connor Tou@connorjtou.bsky.social · 5mo ago

Today in @nature.com we introduce INSTALL, which bypasses mammalian DNA immune sensing to enable non-viral DNA integration with recombinases—a step toward safe, and mutation-agnostic genome editing. 🧬 🧵 (1/13) www.nature.com/articles/s41... @harvardmed.bsky.social @mgbresearch.bsky.social

New online! Immune-evasive DNA integration with INSTALL

A small piece in @natrevgenet.nature.com on our INSTALL technology: Immune-evasive DNA + recombinases for non-viral gene insertion. www.nature.com/articles/s41...

Immune-evasive DNA integration with INSTALL - Nature Reviews Genetics

In this Tools of the Trade article, Connor Tou presents INSTALL, a recombinase-driven, kilobase-scale DNA integration method that employs partially duplexed circular single-stranded DNA to evade the c...

nature.com

Research Highlight: Immune-stealth DNA for large cargo integration www.nature.com/articles/s41...

Genome editing that avoids immune detection to integrate large DNA sequences @nature.com www.nature.com/articles/d41... on paper released last week by @bkleinstiver.bsky.social www.nature.com/articles/s41...

Immune-evasive cssDNA donors combined with recombinases facilitate kilobase-scale insertion of custom genetic cargos in human cells and mice. #NBThighlight www.nature.com/articles/s41...

Immune evasive DNA donors and recombinases license kilobase-scale writing - Nature

INSTALL overcomes fundamental challenges for DNA delivery and integration methods by synergizing immune-stealth nucleic acids with recombinases to enable kilobase-scale integration strategies without ...

nature.com

Nature research paper: Immune evasive DNA donors and recombinases license kilobase-scale writing go.nature.com/4b3e0E2

Immune evasive DNA donors and recombinases license kilobase-scale writing - Nature

INSTALL overcomes fundamental challenges for DNA delivery and integration methods by synergizing immune-stealth nucleic acids with recombinases to enable kilobase-scale integration strategies without viral vectors.

go.nature.com

Today in @nature.com we introduce INSTALL, which bypasses mammalian DNA immune sensing to enable non-viral DNA integration with recombinases—a step toward safe, and mutation-agnostic genome editing. 🧬 🧵 (1/13) www.nature.com/articles/s41... @harvardmed.bsky.social @mgbresearch.bsky.social

Immune evasive DNA donors and recombinases license kilobase-scale writing - Nature

INSTALL overcomes fundamental challenges for DNA delivery and integration methods by synergizing immune-stealth nucleic acids with recombinases to enable kilobase-scale integration strategies without ...

nature.com

Evolution navigated billions of challenges to get to us to where we are today. Directed evolution compresses this to a 1D axis. Imagine if you could sample 200 dimensions at once, with data to boot 📈 First @chorylab.bsky.social PACE preprint on our new system to tackle this: bit.ly/turboprance

🔎 Special report: Gene editing’s toolbox problem. Endpoints Senior Science Correspondent @scienceboss.bsky.social spoke with dozens of biotech leaders and scientists about the promise of new tools — and the growing frustration over how few cures have followed.

Gene editing's search for a 'holy grail' has led to an explosion of tools, but few cures

Former Intellia scientist John Finn reflects on gene editing's evolution and the closure of Tome Biosciences, highlighting the industry's focus on tools over medicines.

endpoints.news

Congrats to IGI alum Connor Tou on being named a 2025 #STATWunderkind! At the IGI, Connor worked on CRISPR EvolvR in John Dueber's lab and now, as a Ph.D. student at MIT, he's working on genome engineering with large insertions.

1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. www.biorxiv.org/content/10.1...

Antiviral reverse transcriptases reveal the evolutionary origin of telomerase

Defense-associated reverse transcriptases (DRTs) employ diverse and distinctive mechanisms of cDNA synthesis to protect bacteria against viral infection. However, much of DRT family diversity remains ...

biorxiv.org

Congrats to @connorjtou.bsky.social on being named a 2025 #STATWunderkind. His enthusiasm in the lab is contagious and he thrives on doing really hard things. Really well deserved recognition for an innovator and emerging leader in our field! 👏 🙌 @mgbresearch.bsky.social @harvardmed.bsky.social

Connor Tou@connorjtou.bsky.social · 10mo ago

Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social. Full list: www.statnews.com/wunderkinds/ @statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social

Taking the stage are three of this year's #STATWunderkinds! Meet the class of 2025: www.statnews.com/wunderkinds/ #STATSummit

At a time when conflict and cynicism seem to be shadowing much of health and medicine, @statnews.com is recognizing some of the brightest young minds in their fields this morning. www.statnews.com/wunderkinds/

Meet the 2025 STAT Wunderkinds

This year, as in past years, we've found inspiring stories and innovative research. All are blazing new trails as they attempt to answer some of the

statnews.com

Honored to be part of the 2025 #STATWunderkinds! Grateful for the many supportive mentors, colleagues, and friends with special shoutout to @bkleinstiver.bsky.social. Full list: www.statnews.com/wunderkinds/ @statnews.com @mitdeptofbe.bsky.social @mgbresearch.bsky.social @harvardmed.bsky.social

How can we understand the earliest events in evolution of eukaryotic immunity? @yao-li.bsky.social reports incredible molecular fossils of complete bacterial-like operons in eukaryotes that illuminate how animal immunity was first acquired from anti-phage defense www.biorxiv.org/content/10.1...

Today in the journal Science: BioEmu from Microsoft Research AI for Science. This generative deep learning method emulates protein equilibrium ensembles – key for understanding protein function at scale. www.science.org/doi/10.1126/...

If you like transposons... If you you love genome editing... Or if you just like random bird animations, we have the paper for you! We (@kedmonds.bsky.social et al) are happy to share our work turning a songbird retrotransposon into a genome editing tool. 🐣 (1/n)

Click editing uses DNA polymerases, HUH endonucleases and oligonucleotide templates for genome modification go.nature.com/3zPEIjp rdcu.be/erfhL

Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases - Nature Biotechnology

Click editing uses DNA polymerases, HUH endonucleases and oligonucleotide templates for genome modification.

go.nature.com

1/10 New pre-print(s) from the Sternberg Lab in collaboration with Leifu Chang's Lab! We uncover the unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs. Full story here: www.biorxiv.org/content/10.1...

1/10 Today in @science.org in collaboration with the Liu group we report the development of a laboratory-evolved CRISPR-associated transposase (evoCAST) that supports therapeutically relevant levels of RNA-programmable gene insertion in human cells. drive.google.com/file/d/1I-Ub...

So excited to share our new paper out today in @nature.com. We repurposed R2 retrotransposons to create STITCHR, a tool that can make virtually any kind of edit in mammalian genomes including the scarless multi-kilobase scale insertions 🧵 www.nature.com/articles/s41...

Reprogramming site-specific retrotransposon activity to new DNA sites - Nature

A study of retrotransposon activity repurposes a retroelement called R2Tocc to create a programmable system called STITCHR that enables diverse genome edits including efficient, scarless large pa...

nature.com

Interested in learning more about genome editing tool dev & their application to treat human diseases? 🧬 🏥 We're hiring graduating seniors who are seeking ~2 years of post-bac research experience. If you're interested in gaining experience in next gen medicines, please reach out and apply! Link 👇