After a long review process, I'm excited that our paper is finally in print: www.cell.com/cell/fulltex... TL;DR: We use CRISPR screens in iPSC-derived neurons to find a new tau E3 ligase and a relationship between oxidative stress, the proteasome, and tau proteolytic fragments. More below 👇

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

CRISPR screens in iPSC-derived neurons reveal that the E3 ubiquitin ligase CRL5SOCS4 ubiquitinates tau, that CUL5 expression is correlated with resilience in human Alzheimer’s disease, and that electr...

cell.com

Zara Weinberg passed away earlier this week. She joined my lab earlier this year when her postdoc lab formally closed. She was a brilliant scientist, supportive mentor, dear friend, avid music fan, rabid believer in public transit, open science champion, and above all just an amazing human.

Finally out! I’m thrilled to share our new paper (Wolin et al., Cell 2025). This paper describes SPIDR, a high-throughput method for mapping RBP binding sites. By combining #SPIDR with #cryoEM, we identified the exact binding site of LARP1 within the #mRNA channel of the 40S ribosomal subunit.

Hey that protein looks familiar! 🦋

Alexey Amunts@amunts.bsky.social · last yr.

Speak up to prevent a major loss for the field. CASP, which helped shaping structural biology including AlphaFold is on the verge of shutting down. NIH funding has lapsed, UC Davis support ends and the core team is being let go. Painfully shortsighted.

Speak up to prevent a major loss for the field. CASP, which helped shaping structural biology including AlphaFold is on the verge of shutting down. NIH funding has lapsed, UC Davis support ends and the core team is being let go. Painfully shortsighted.

Exclusive: Famed protein structure competition nears end as NIH grant money runs out

Agency silent on funding renewal for contest that inspired creation of AIs that predicted how proteins would fold

science.org

I'm excited to share our latest story from the @lydiafinley.bsky.social lab, where we show that it's not just whether cells take up nutrients that matters, but how and where those metabolites are used. Out now in @naturemetabolism.bsky.social: rdcu.be/ejFTs 1/

Intracellular metabolic gradients dictate dependence on exogenous pyruvate

Nature Metabolism - Jackson et al. provide insight into how metabolic adaptations that accompany cell state transitions drive reliance on exogenous nutrient availability, focusing on pyruvate as a...

rdcu.be

Happy that my first paper from my Postdoc is out where we perform Phospho-seq, a single-cell ATAC-sequencing with intracellular protein quantification in human brain organoids: www.nature.com/articles/s41...

Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells - Nature Communications

Here, the authors demonstrate Phospho-seq, a single-cell multiomics method capable of quantifying chromatin accessibility alongside intracellular proteins, including post-translationally modified prot...

nature.com

So excited to co-chair this session with @herrflow.bsky.social tomorrow! Come hear cool talks ranging from computational to experimental approaches to understand protein dynamics and allostery!

Steffen Wolf@herrflow.bsky.social · 2y ago

If you are interested in all things concerning protein allostery and are at #BPS2025: come to the Platform: Protein Dynamics and Allostery I chaired by @rosalielawrence.bsky.social and me Monday morning at 10:45 h in room 515B! l.core-apps.com/bpsam2025/ev...

Attending #BPS2025? Want to know more about tangible steps you can take to challenge the attacks on science in the U.S.? Please attend an Emergency Town Hall Meeting on Tuesday at 1:30! @biophysicalsoc.bsky.social @blackinbiophys.bsky.social Please spread the word!

Great news, great opportunity for students and postdocs!!

George Ghanim@automnenine.bsky.social · 2y ago

After many years at @mrclmb.bsky.social, I've moved back home to start my own lab at @princetonmolbio.bsky.social. I'm so thankful to my mentors, especially Kelly Nguyen and Don Rio! We have some exciting results on LINE-1 in the works and are recruiting. Find out more: ghanim.lab.princeton.edu

First post on bluesky gotta be the structure of mTORC1 on membrane! A great collaboration and interesting frontier for SPA cryoEM!

Jim Hurley@jimhurley.bsky.social · 2y ago

Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/ G. Napolitano,A. Esposito, and A. Ballabio.

If you don’t feel stupid doing science, you’re not trying hard enough.

Our lab is hiring a postdoc! We have multiple projects in uterine, placental, and pregnancy biology. Candidates with a background in stem cells, development, reproduction, or bioinformatics are welcome to apply. See the attached ad and visit zhang.faculty.ucdavis.edu for more info.

The Lawrence Lab website (lawrence-lab.org) is live and we are hiring for our launch in Jan 2025! We combine high resolution structure 🔬 and cell biology 🧪🧫 approaches to define mechanisms of stress response signaling in health and disease. Live postings below--Please RT and send to recent grads!

Our updated preprint is live! www.biorxiv.org/content/10.1... @kampmann.bsky.social We add new data: 1. A new CRL5 complex that targets tau for degradation 2. CUL5 as a marker of vulnerability in Alzheimer's Disease 3. Oxidative stress-derived tau fragments change in vitro tau aggregation

CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis

Aggregation of the protein tau defines tauopathies, which include Alzheimer's disease and frontotemporal dementia. Specific neuronal subtypes are selectively vulnerable to tau aggregation and subseque...

biorxiv.org

Thanks @tlnagy.bsky.social ! I'm excited to join the UCLA community 😊

Tamas Nagy@tamasnagy.com · 2y ago

Great talk today by @rosalielawrence.bsky.social on her background and her future lab plans 🎉

Are you at #Cellbio2023? Let's hang out! I'll be speaking in the Cryo-EM and Cryo-ET in the Discovery of Cellular Mechanisms session about the eIF2B Switch-Helix on Monday at 5:30, and have a poster (P2953) on Tuesday at 1:45! bsky.app/profile/rosa...

It’s out! Via hydrogen deuterium exchange, biochemistry, and cryo-EM, we (Peter Walter lab, Susan Marqusee lab et al) discovered the allosteric mechanism controlling activity of the core Integrated Stress Response (ISR) regulator eIF2B. 1/10 www.nature.com/articles/s41...