The #devbio project that encompassed the majority of my postdoc is finally out! Huge thanks to my coauthors and our collaborators without whom this work would not have been possible, and my adviser Eszter Posfai who let me play with a light sheet microscope and some colorful mice :)

Eszter Posfai@eposfai.bsky.social · last yr.

Thrilled to share our work using live imaging to understand how Epiblast (future embryo proper) and Primitive Endoderm (future extraembryonic tissues) cell fates segregate in the preimplantation mouse embryo. Gargantuan effort led by amazing @rpkimyip.bsky.social, David Denberg and Denis Faerberg!

Thrilled to share our work using live imaging to understand how Epiblast (future embryo proper) and Primitive Endoderm (future extraembryonic tissues) cell fates segregate in the preimplantation mouse embryo. Gargantuan effort led by amazing @rpkimyip.bsky.social, David Denberg and Denis Faerberg!

pYtags - our in vivo biosensors for receptor tyrosine kinases - in all their beauty on the cover of Cell Reports! Read more here: www.cell.com/cell-reports...

A duo of preprints on the dynamics of the first cell fate decision in mouse by Madeleine Chalifoux (first grad student in the lab!) and Maria Avdeeva (Flatiron). We use quantitative live imaging of key cell fate determinants to follow the segregation of inner cell mass and trophectoderm lineages.

Have you ever wanted to *see* receptor activity in embryos? If so, our new preprint is for you! In it, we showcase a new live-cell biosensor for visualizing receptor tyrosine kinase activity in living embryos – pYtags! www.biorxiv.org/content/10.1...

Happy Fly Day! November 18th is #Drosophila day. It commemorates the isolation of a white-eyed mutation, which is now used across the world. That mutation is white[1118], with the numbers standing for Nov 18th, the day it was isolated by Bob Levis. 🪰

Looking for a tag to localise fluorescent proteins to the plasma membrane? Unsure which tag might work in your species? We generated a toolkit of 11 membrane-localising tags, which can be screened rapidly by microinjecting mRNA in your species of interest. 1/4 www.biorxiv.org/content/10.1...

A toolkit for testing membrane localisation tags across species

Transgenic markers and tools have revolutionised how we study cells and developing organisms. Some of the elements needed to construct those tools are universally applicable (e.g. fluorescent proteins...

biorxiv.org

For those leaving Twitter: my postdoctoral work uses live imaging to understand early embryos. Check out our recently (!) published paper in Development to see the imaging and quantification we can do #devbio #liveimaging #lightsheet #embryos journals.biologists.com/dev/article/...

Nuclear instance segmentation and tracking for preimplantation mouse embryos

Summary: Image analysis pipeline for 3D nuclear instance segmentation and lineage tracking for preimplantation mouse embryos, including description of new H2B-miRFP720 reporter line and a large ground...

journals.biologists.com

Let's try this and bring back some good old twitter vibes #ICanHazPDF www.nature.com/articles/s43...

Fluorescence lifetime imaging microscopy - Nature Reviews Methods Primers

Fluorescence lifetime imaging microscopy can offer insights into biological processes such as metabolic imaging, protein–protein interactions and live-cell intracellular dynamics. In this Primer, Torr...

nature.com

by way of intro (and since 'tis almost the season) here's my developmental biology holiday sweater I knit during the pandemic. Vertebrates on one side, inverts on the other, all charts designed by me. #devbio #knitting