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Carsten Sachse

@sachsegroup.bsky.social

The Sachse group @fz_juelich: #cryoEM #cryoET #CLEM #FIBSEM #autophagy.

5 Posts529 Follower258 Folgt
Carsten Sachse@sachsegroup.bsky.social · 8mo ago

We are looking for a team leader to join the cryo-EM facility work at the new Ernst Ruska-Centre 2.0 at the Forschungszentrum Jülich. We have a series of microscopes used by (@irene-vercellino.bsky.social, Schröder, @sachsegroup.bsky.social) and external users. www.fz-juelich.de/de/karriere/...

Team Leader – Life Science Cryo-Electron Microscopy (Cryo-EM) Facility

Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C) at Forschungszentrum Jülich houses some of the world`s most advanced electron microscopes and tools for nanocharacterisation. U...

fz-juelich.de

Carsten Sachse@sachsegroup.bsky.social · 8mo ago

Really happy to see the work (doi.org/10.1038/s414...) published. Here, we show the full-length helical cryo-EM structure of p62/SQSTM1 and first cryo-ET visual of p62 positive structures inside the cell.

Structural organization of p62 filaments and the cellular ultrastructure of calcium-rich p62-enwrapped lipid droplet cargo - Nature Communications

The selective autophagy receptor p62/SQSTM1 is organized in a structured double helical filament in vitro, the cryo-electron tomographic images in ATG5 knockdown conditions reveal Ca-rich layers of p6...

doi.org

Tanmay Bharat@tbharat-lab.bsky.social · last yr.

Finally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.

PLOS Biology@plosbiology.org · last yr.

The unique #CellEnvelope of #corynebacteria: @tbharat-lab.bsky.social &co map the C. glutamicum cell surface, revealing a patchy S-layer & specific assembly of the PS2 protein, thereby informing our understanding of cell envelopes that contain #MycolicAcids @plosbiology.org 🧪 plos.io/3Y6JNgw

Left: The atomic model of the PS2 lattice is shown in top and side views. PS2 hexamers are repeated in the 2D sheet with a lattice constant of 176 Å. Each PS2 monomer is colored and labeled separately within the hexamer, which repeats throughout the lattice (with pore dimensions highlighted). The side view of the lattice shows the smooth surface facing the extracellular space and rough surface with coiled-coil segments protruding towards the MM. Top right: Interactions at the hexameric and trimeric interfaces are stabilized by both hydrophilic and hydrophobic residues. Bottom right: A cell envelope tomogram was used to overlay the cryo-EM structure of the S-layer onto the in situ S-layer tomographic density. A single slice of the tomogram is shown, with the overlayed PS2 S-layer lattice in green and the tomogram in grayscale. The S-layer (surface layer), MM (mycomembrane), IM (inner membrane), and Cytosol are labeled.
Josh Dickerson@joshdcryoem.bsky.social · last yr.

After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it. 🔗 www.pnas.org/doi/10.1073/...

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS

The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

pnas.org

Carsten Sachse@sachsegroup.bsky.social · last yr.

Philipp's manuscript on our CryoVIA toolkit www.sciencedirect.com/science/arti... is out! For those interested in studying protein lipid interactions and high-throughput analysis of membrane structures (sizes, shapes, curvature etc.) - this could be your tool (github.com/philipp-scho...).

CryoVIA: An image analysis toolkit for the quantification of membrane structures from cryo-EM micrographs

Imaging of lipid structures and associated protein complexes using cryoelectron microscopy (cryo-EM) is a common visualization and structure determina…

sciencedirect.com

Carsten Sachse@sachsegroup.bsky.social · 2y ago

Our first post on blue: grad student Philipp's software: for those interested in looking at membrane reconstitution by cryo-EM. Automated analysis of lipid fine structure and membrane shape classifications: biorxiv.org/cgi/content/...

CryoVIA - An image analysis toolkit for the quantification of membrane structures from cryo-EM micrographs

Imaging of lipid structures and associated protein complexes using electron cryo-microscopy (cryo-EM) is a common visualization and structure determination technique. The quantitative analysis of the ...

biorxiv.org

Irene Vercellino@irene-vercellino.bsky.social · 2y ago

Excited to share that I have been elected as member of the "Junge Kolleg" of the Nordrhein-Westfalian Academy of Sciences and Arts. I am honoured to have this opportunity to serve the community! Here's the link to the announcement, unfortunately only in German www.awk.nrw/news/nachwuc...

Nachwuchsförderung: Nordrhein-Westfälische Akademie nimmt neun neue Stipendiatinnen und Stipendiaten in ihr Junges Kolleg auf

Zum 1. Januar 2025 begrüßt die Nordrhein-Westfälische Akademie der Wissenschaften und der Künste neun weitere herausragende Talente in ihrem Jungen Kolleg. Sie haben die Jury in einem mehrstufigen Aus...

awk.nrw