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Tanmay Bharat

@tbharat-lab.bsky.social

MRC-LMB #cryoET #biofilms #S-layers #antibiotics https://www2.mrc-lmb.cam.ac.uk/groups/bharat/

59 Posts1449 Follower627 Folgt
Matthew Freeman@mjafreeman.bsky.social · 7d ago

Yet another opportunity @dunnschool.bsky.social! We are looking for an Associate Professor If you have exciting research plans, @dunnschool.bsky.social is a great place: excellent science (and lovely people, obvs) www.path.ox.ac.uk/vacancy/asso...

Associate Professorship of Cancer Biology - Sir William Dunn School of Pathology in association with the Queen’s College. - Dunn School

We are seeking to appoint an Associate Professor working in the area of the cellular and/or molecular biology of cancer, to be based in Oxford University’s Sir William Dunn School of Pathology. The ro...

path.ox.ac.uk

Sjors Scheres@sjorsscheres.bsky.social · last wk.

Our adventures into #proteinfolding: GuideFlip designs flexible molecular interactions by discrete flow matching to design sequence & structure together! Grateful for funding by Love Tito's (@titosvodka.bsky.social) and the @ukri.org. 🥳

Kai Yi@kaiyi94.bsky.social · last wk.

1/7 Excited to share GuideFlip! We use guided discrete flow matching to co-design protein sequences and structures for flexible interactions, with experimental validation across three systems: α-synuclein, RBX1 and active-state GPCR nanobodies.

Kai Yi@kaiyi94.bsky.social · last wk.

1/7 Excited to share GuideFlip! We use guided discrete flow matching to co-design protein sequences and structures for flexible interactions, with experimental validation across three systems: α-synuclein, RBX1 and active-state GPCR nanobodies.

Joe Greener@jgreener64.bsky.social · 3w ago

Our Garnet paper is now out in final form: a protein and small molecule force field trained from scratch, with competitive results for binding free energy prediction. pubs.rsc.org/sc/article/d...

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pubs.rsc.org

Joe Greener@jgreener64.bsky.social · 7mo ago

Check out our pre-print, where we train a protein and small molecule force field from scratch with a graph neural network. We show comparable performance to existing, manually-tuned force fields on a range of tasks including binding free energy prediction. (1/4) arxiv.org/abs/2603.16770

Julian Parkhill@julianparkhill.bsky.social · 3w ago

This will be an amazing opportunity for the right person. The Department of Veterinary Medicine in Cambridge is recruiting for an established chair who will also be our Head of Department. Your research can be in any area of veterinary science. Please circulate. www.cam.ac.uk/jobs/profess...

Professorship of Veterinary Science

The Board of Electors to the Professorship of Veterinary Science invite applications for this Professorship from persons whose work is connected with veterinary science (ideally in research aligned wi...

cam.ac.uk

Sjors Scheres@sjorsscheres.bsky.social · 3w ago

Michel Goedert and myself are looking for two postdocs to work on #cryoEM and #masspec of #alphasynuclein filaments from neurodegenerative disease. Come join our bubbly and friendly team at the @mrclmb.ac.uk in lovely Cambridge, UK. www.nature.com/naturecareer...

Postdoctoral Scientist | Neurobiology | Dr Michel Goedert | LMB 2842 - Cambridgeshire job with MRC Laboratory of Molecular Biology | 12864505

Postdoctoral Scientist Salary £42,694 per annum Fixed term, 3 years MRC Laboratory of Molecular Biology, Cambridge, UK To work in the group of Dr M...

nature.com

Tanmay Bharat@tbharat-lab.bsky.social · 4w ago

Exciting work by @florianmayer.bsky.social, @buzzbaum.bsky.social and colleagues. Archaea with more than one membrane! cryo-FIB-SEM volume imaging from Andriko von Kügelgen and @zhexinwang.bsky.social.

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Florian Mayer@florianmayer.bsky.social · 4w ago

Excited to share our preprint on Ignicoccus cell divison: only the inner membrane divides, progeny cells accumulate in cell clusters and pop-out! www.biorxiv.org/content/10.6... Great work with the labs: @buzzbaum.bsky.social @curiousdina.bsky.social @anja1.bsky.social @tbharat-lab.bsky.social

Matthew Freeman@mjafreeman.bsky.social · 4w ago

Less than 2 weeks to apply! If you are looking for early career fellowship sponsorship, consider @dunnschool.bsky.social to launch your independent career Great science, great support and, of course, lovely colleagues... www.path.ox.ac.uk/work-with-us...

Group Leader Career Development Fellowships - Dunn School

Are you an early career researcher interested in the cell or molecular mechanisms underlying disease? Do you have an outstanding record and an innovative research plan?

path.ox.ac.uk

The EMBO Journal@embojournal.org · 2mo ago

What’s the function of embryonic ‘cytoplasmic lattices’? Using cryo-ET, @kashishsingh.bsky.social @carter-lab.bsky.social and coworkers reveal them as megadalton-size hubs of ubiquitination proteins/ubiquitin transfer link.springer.com/article/10.1...

In-situ cryo-ET of mouse embryos reveals cytoplasmic lattices contain ubiquitin-charged E2-E3 ligase assemblies - The EMBO Journal

Cytoplasmic lattices (CPLs) are filamentous assemblies essential for mammalian embryonic development. They are known to regulate organelle organization, spindle assembly, and protein homeostasis, but ...

link.springer.com

Rob Fagan@robfagan.bsky.social · 2mo ago

The latest from us as part of a special issue on S-layers organised by @tbharat-lab.bsky.social S-layers make ideal phage receptors but present another barrier that must be overcome to deliver the genome into the cytoplasm. We know very little about phage that can do this!

@perbullough.bsky.social · 2mo ago

www.sciencedirect.com/science/arti... Our latest paper on C. difficile phage/S-layer interactions with thanks to @robfagan.bsky.social @jasonwils.bsky.social for all their hard work on this.

Admirable Women@admirablewomen.bsky.social · 2mo ago

Chemist & X-ray crystallographer Rosalind Franklin's meticulous research was instrumental in uncovering DNA's molecular structure. Most famous for her role in the DNA double helix discovery, her work also revolutionized our understanding of viruses & coal. She was born #OTD in 1920. #WomenInSTEM 🧵

A classic, black-and-white studio portrait of a young Dr. Rosalind Franklin (1920–1958), the pioneering British chemist and X-ray crystallographer whose data was foundational to uncovering the double-helix structure of DNA. Captured from the chest up, Franklin sits in a three-quarter pose, her body angled slightly toward the left while her gaze directly meets the viewer. She possesses a calm, intelligent, and subtly enigmatic expression, with her dark hair neatly parted on the side and styled back away from her face. She wears a dark, long-sleeved button-down blouse with structured shoulders, a sharp collar, and fabric buttons down the front placket. The background is a soft, minimalist studio gradient of neutral grays that creates a gentle vignette effect, casting a soft light on the right side of her face. The composition is clean, formal, and balanced, evoking a mood of quiet determination, dignity, and brilliant intellectual poise characteristic of the early 20th-century scientific vanguard.
Helen Branswell 🇨🇦@helenbranswell.bsky.social · 3mo ago

Have you ever seen this iconic image of the Ebola virus, from the first known outbreak in DRC in 1976? An electron microscopic image, it was captured by #CDC 's Fred Murphy, a virological giant and a very kind interviewee. He died yesterday. He will be missed, but his scientific legacy lives on.

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Tanmay Bharat@tbharat-lab.bsky.social · 4mo ago

Disrupting phage liquid crystalline droplets restores antibiotic susceptibility in Pseudomonas aeruginosa biofilms out in @plosbiology.org by @abultarafder.bsky.social and team. Exciting collaboration with @geiselbiofilm.bsky.social @pearce-maths.bsky.social and others

PLOS Biology@plosbiology.org · 4mo ago

#Biofilm matrices containing filamentous phages help #Pseudomonas aeruginosa tolerate antibiotics. @abultarafder.bsky.social @tbharat-lab.bsky.social &co show that #nanobody disruption of #phage Pf4 #LiquidCrystalline droplets restores #antibiotic susceptibility @plosbiology.org 🧪 plos.io/4xkd6Mw

Top: Nanobody binders are potent inhibitors of Pf4 liquid crystalline droplet formation and disrupt preformed droplets. Cryo-ET of Pf4 liquid crystalline droplets incubated with Nb43. Tomographic slice of a Pf4 liquid crystalline droplet specimen incubated with (left) 0.1 μM and (right) 1 μM Nb43. Bottom: Schematic representation of nanobody action in abolishing antibiotic tolerance of P. aeruginosa biofilms. In untreated biofilms (left), cells show increased antibiotic tolerance due to Pf4 liquid crystalline droplets formed by depletion attraction in the biofilm EPS matrix, where encapsulated cells are protected by an antibiotic diffusion block. In nanobody treated biofilms, patchy binding of nanobody to Pf4 filaments reduces depletion attraction between the filaments preventing liquid crystalline droplet formation and encapsulation of cells, leading to increased antibiotic susceptibility of bacteria within the biofilm.
Tanmay Bharat@tbharat-lab.bsky.social · 5mo ago

Applications and prospects of cryo-electron tomography in drug discovery and understanding disease Review article by @camilaclemente.bsky.social in our lab www.sciencedirect.com/science/arti...

Applications and prospects of cryo-electron tomography in drug discovery and understanding disease

Cryo-electron tomography (cryo-ET) is emerging as a transformative tool for structural biology. Unlike methods based on purified molecules, cryo-ET en…

sciencedirect.com

Tanmay Bharat@tbharat-lab.bsky.social · 5mo ago

Interaction of Pf4 tactoids with bacteria and synthetic colloidal rods Important biophysical measurements on the Pseudomonas aeruginosa biofilm-related phage Pf4 by Dirk Aarts laboratory with @abultarafder.bsky.social link.springer.com/article/10.1...

Interaction of Pf4 tactoids with bacteria and synthetic colloidal rods - European Biophysics Journal

We investigated the interaction of liquid crystal droplets, formed by a phase separating system of elongated virus particles and polymer with rod-like impurities. The virus particles were Pf4, which w...

link.springer.com

Tanmay Bharat@tbharat-lab.bsky.social · 5mo ago

Oxygen gradients reshape cross-feeding through emergent spatial organization of gut commensal bacteria www.biorxiv.org/content/10.6... Use of isotope labels and cryo-CLEM-FIB-SIMS to study microbial communities by Hannah Ochner. Collaboration with @kiranrpatil.bsky.social @jmghigolab.bsky.social

Oxygen gradients reshape cross-feeding through emergent spatial organization of gut commensal bacteria

Microbial interactions unfold within environments structured by physical transport and chemical gradients. Yet most mechanistic studies rely on well-mixed systems that mask the reciprocal influences of environmental heterogeneity on metabolism and ecology. Here, we investigate how the physical environment modulates the interaction between the gut commensal Bacteroides thetaiotaomicron and Escherichia coli . In anoxic liquid culture, cell-resolved isotope imaging and genetic perturbations reveal exploitative cross-feeding, where E. coli consumes diffusible sugars released by B . thetaiotaomicron during starch degradation. When exposed to intestinal-like oxygen gradients in microfluidics, the interaction is restructured by spatial organization. The species self-organize into complementary niches: E. coli locally depletes sugars and oxygen, thereby expanding the anoxic niche required by B. thetaiotaomicron . A reactive transport model confirms that this organization arises from coupled feedback between physical transport and metabolic reaction rates. Together, our results reveal how physical structure and chemical gradients convert an exploitative cross-feeding interaction into a dynamic niche-construction process that generates emergent spatial organization and stabilizes coexistence. ### Competing Interest Statement The authors have declared no competing interest.

biorxiv.org