Now our lab is NIH funded. This funding gives us real stability and room to keep building on the questions we care about. I am proud of what my team has accomplished and look forward to what lies ahead. Huge thanks to my mentors and #NIGMS.
Pabi Sahoo
@sahoolab.bsky.social
Assistant Professor at Rutgers University | Molecular Neurobiologist | Neural Repair | Neurodevelopment
Read this exciting work from the @kristywelshhans.bsky.social lab led by Manasi Agrawal. Very happy to have contributed to this story. Restoring DSCAM expression mitigates neuronal morphology and axon guidance deficits in Down syndrome academic.oup.com/brain/articl...
Restoring DSCAM expression mitigates neuronal morphology and axon guidance deficits in Down syndrome
Using human and mouse models of Down syndrome, Agrawal et al. show that overexpression of the chromosome 21 gene DSCAM disrupts neuronal development and ax
academic.oup.com
Finally out in @Cellcellpress! Proteins with long IDRs are prone to misfolding during protein synthesis. This is prevented by mRNA 3′UTRs that act as mRNA-based IDR chaperones. www.cell.com/cell/fulltex...
mRNA 3′ UTRs chaperone intrinsically disordered regions to control protein activity
Highly conserved mRNA 3′ UTRs act as co-translational chaperones for intrinsically disordered regions (IDRs), preventing inter-domain misfolding and enabling biogenesis of fully active proteins.
cell.com
www.nature.com/articles/s41...
Stress granules restrain ferroptosis by sequestering ferritin - Nature Cell Biology
Ge, Wang, Zhao, Guo et al. report a role for stress granules in suppressing ferroptosis by sequestering ferritin upon irradiation and temozolomide treatment in glioblastoma stem cells, which may poten...
nature.com
First major grant for the lab. Thank you, #NJCBIR, for believing in us. Now let's get back to the bench to get that work done :)
🚨 New pre-print! If you are interested in new roles for SARM1, check this out. The lab did cool work showing macrophages need Sarm1 to efficiently respond to immunoogical stimuli and PNS injury. Cell specific sarm1 KO mice give surprising results- check it out! www.biorxiv.org/content/10.6...
biorxiv.org
link.springer.com/article/10.1...
Proteolytic cleavage of G3BP1 by calpain 1 couples NMDAR activation to mTOR-dependent local translation - EMBO Reports
Ribonucleoprotein (RNP) granules are dynamic, membraneless organelles that sequester translationally repressed mRNAs and RNA-binding proteins, playing a pivotal role in the regulation of localized pro...
link.springer.com
🧪 When an mRNA has not one or two, but at least THREE localization signals, suggesting that mTOR mRNA requires robust localization. See our preprint, lead by Nitzan Samra with greatly appreciated collaborations with @sahoolab.bsky.social and the Jeffery L. Twiss lab www.biorxiv.org/content/10.6...
biorxiv.org
How many axon localization elements are in one mRNA? mTor mRNA seems to have at least 3. Read this interesting preprint from the @mfainzilber.bsky.social lab. Multiple mTOR RNA localization signals regulate subcellular protein synthesis and axonal growth www.biorxiv.org/content/10.6...
biorxiv.org
There is a current push towards doing biological research using only hiPSC models, but are we there yet? Manasi nicely discusses it in this review using neural repair as a model system. www.sciencedirect.com/science/arti...
Human induced pluripotent stem cell-based models for studying neural repair
Injuries and degenerative diseases of the human nervous system result in irreversible functional loss, reflecting the limited regenerative capacity of…
sciencedirect.com
So it was an RNA world 🤔 A small polymerase ribozyme that can synthesize itself and its complementary strand | Science www.science.org/doi/10.1126/...
A small polymerase ribozyme that can synthesize itself and its complementary strand
The emergence of a chemical system capable of self-replication and evolution is a critical event in the origin of life. RNA polymerase ribozymes can replicate RNA, but their large size and structural ...
science.org
Very happy to share our new paper in #ScienceSignaling revealing that importin β1 isn’t just important for nuclear transport or neuronal growth. We now show it is key for presynaptic plasticity in the hippocampus, local translation, and memory! www.science.org/doi/10.1126/...
Subcellular depletion of importin β1 impairs presynaptic local translation and spatial memory
Axonal localization of importin β1 is required for presynaptic functions that support spatial memory tasks.
science.org
🧪 An EMBO Meeting on Axon Biology in Okinawa, Japan Iconic science at an amazing venue. Please join us! EMBO | COB Workshop on Axonal degeneration and regeneration meetings.embo.org/event/26-axo...
Axonal degeneration and regeneration
Neurons are the largest and most long-lived class of cells, necessitating specialized mechanisms for their survival, growth and maintenance. Axons are the longest extensions of a neuron, reaching len…
meetings.embo.org
ATP - not only a fuel source! Intriguing work from Marco Terenzio and colleagues at the OIST www.science.org/doi/full/10....
Loss of intracellular ATP affects axoplasmic viscosity and pathological protein aggregation in mammalian neurons
The protective role of ATP in neurons reduces protein aggregation and neurodegeneration.
science.org
Excellent story from the Woolf lab! www.nature.com/articles/s41...
Non-muscle myosin II inhibition at the site of axon injury increases axon regeneration - Nature Communications
Therapeutic interventions for motor axon regeneration following peripheral nerve injury are currently unavailable. Here authors show local administration of a non-muscle myosin II inhibitor at the inj...
nature.com
Pretty cool!
Mike Fainzilber@mfainzilber.bsky.social · last yr.“In neurons, RiboExM revealed a functional dichotomy between monosomes and polysomes. Monosomes were enriched in distal neurites at baseline, whereas polysomes formed dynamically in response to external stimuli…” www.science.org/doi/10.1126/...
New preprint from Roz Segal’s group on specialization in local axonal translation www.biorxiv.org/content/10.1...
Profiling local translatomes and RNA binding proteins of somatosensory neurons reveals specializations of individual axons
Individual neurons have one or more axons that often extend long distances and traverse multiple microenvironments. However, it is not known how the composition of individual axons is established or l...
biorxiv.org
Our CNS regeneration work has now been published in @PNAS. It was a huge undertaking in collaboration with @Twiss @Benowitz @WardLab_Emory @KristyWelshhans @Marktuszynski @jenniferDulin @ArthurEnglish @JohnHolule labs. Thank you all, and @MerkinPnnrCtr, for funding. www.pnas.org/doi/10.1073/...
Disruption of G3BP1 granules promotes mammalian CNS and PNS axon regeneration | PNAS
Depletion or inhibition of core stress granule proteins, G3BP1 in mammals and TIAR-2 in Caenorhabditis elegans, increases the growth of spontaneous...
pnas.org
First peer reviewed article from our lab is now out. If you are new to stress granule field, you will find this manuscript helpful. Big shout out to @Meghal Desai @keyagulati @Manasi Agrawal, @Shruti Ghumra Thanks @MerkinPnnrCtr for funding 🙏🏼 journals.lww.com/nrronline/ab...
Stress granules: guardians of cellular health and triggers... : Neural Regeneration Research
play a critical role in minimizing cellular damage during stress. Composed of heterogeneous ribonucleoprotein complexes, stress granules are enriched not only in mRNAs but also in noncoding RNAs and v...
journals.lww.com
www.biorxiv.org/content/10.1...
Context-specific interaction of the lipid regulator DIP-2 with phospholipid synthesis in axon regeneration and maintenance
Neurons maintain their morphology over prolonged periods of adult life with limited regeneration after injury. C. elegans DIP-2 is a conserved regulator of lipid metabolism that affects axon maintenan...
biorxiv.org
New study from the Twiss lab! www.biorxiv.org/content/10.1...
KHSRP-mediated Decay of Axonally Localized Prenyl-Cdc42 mRNA Slows Nerve Regeneration
The small GTPase CDC42 promotes axon growth through actin filament polymerization and this growth is driven by axonal localization of the mRNA encoding the prenylated CDC42 isoform ( Prenyl-Cdc42 ). H...
biorxiv.org
Synthetic biomolecular condensates enhance translation from a target mRNA in living cells www.nature.com/articles/s41...
Synthetic biomolecular condensates enhance translation from a target mRNA in living cells - Nature Chemistry
Formation of biomolecular condensates composed of proteins and RNA facilitates the regulation of gene expression by modulating translation or facilitating RNA processing. Now, synthetic ribonucleoprot...
nature.com
www.science.org/doi/10.1126/...
Protein codes promote selective subcellular compartmentalization
Cells have evolved mechanisms to distribute ~10 billion protein molecules to subcellular compartments where diverse proteins involved in shared functions must assemble. Here, we demonstrate that prote...
science.org
G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration: Cell Reports www.cell.com/cell-reports...
G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration
Boraas et al. identify that mRNAs and the stress granule RNA-binding protein G3BP1 form ribonucleoprotein (RNP) complexes at FAs. These RNPs regulate FA protein mobility and cell migration under norma...
cell.com
Molecular determinants of condensate composition: Molecular Cell www.cell.com/molecular-ce...
Molecular determinants of condensate composition
Biomolecular condensates are non-stochiometric assemblies that organize cellular matter in space and time. Despite much work, many questions remain regarding what determines the recruitment and exclus...
cell.com