Spoiler: lots of 😍 SPY555-FastAct live cell movies in there.

Journal of Cell Biology@jcb.org · 2mo ago

Our June issue is here! rupress.org/jcb/issue/22... The cover shows a maximum intensity projection from a confocal image stack showing an angiogenic sprout emerging from an engineered microvessel. From @lakynm.bsky.social et al. of the Matthew Kutys lab: rupress.org/jcb/article/... #Cytoskeleton

Today is the Day, CenSpark650 is available! Time to get those centrioles and cilia shining under the 🔬objective! spirochrome.com/product/cens... video: 20h imaging of primary cilium formation in a single RPE-1 cell stained with CenSpark650. movie credits: @cepourroy.bsky.social

An amazing new tool to explore "non-models" is just about to open a new world of imagining possibilities....

Spirochrome@spirochrome.com · 2mo ago

📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/ #Bioimaging

📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/ #Bioimaging

Casually posting this one for no particular reason! #MayTheFourth

Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆 The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes. evidentscientific.com/en/landing/i...

#FluorescenceFriday material here!

Spirochrome@spirochrome.com · 3mo ago

Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆 The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes. evidentscientific.com/en/landing/i...

Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆 The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes. evidentscientific.com/en/landing/i...

What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...

Mesmerizing light sheet imaging of neutrophils swarming at a yeast target! The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue). This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading! www.biorxiv.org/content/10.6...

What a probe! Congratulations @cepourroy.bsky.social! We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!

Cédric Pourroy@cepourroy.bsky.social · 4mo ago

CenSpark story is now published in @natchembio.nature.com. We engineered a dual-ligand fluorescent probe to selectively "light up" centrioles & cilia. Massive thank to Pierre Gönczy, @lreymond.bsky.social and @ghatzopoulos.bsky.social for the guidance. Check it out 👉: doi.org/10.1038/s415...

Organoids stained with Spirochrome probes for actin and DNA! 😍

Valentin Dunsing-Eichenauer@dunsingvalentin.bsky.social · 4mo ago

Painted 100+ lung organoids for Easter, stained with live-cell Cell Tracker/ @spirochrome.com dyes, imaged at our @leicamicrosystems.bsky.social Viventis Deep light-sheet 🔬 at Charité (soon @Si-M - Der Simulierte Mensch) and visualized using inverted lookup tables designed by @christlet.bsky.social

See how PKMito dyes promote mitochondrial research😀 @spirochrome.com

Juan C. Landoni@jclandoni.bsky.social · 4mo ago

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

Beware the SPY488-FastAct rollercoaster! We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct. Find out more about SPY488-FastAct: spirochrome.com/product/spy4...

📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy! There is now a very detailed #JOVE video that explains it all step by step. Video link: dx.doi.org/10.3791/6940... Peroxi_SPY link: spirochrome.com/products/?_c... 🧪

📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy! There is now a very detailed #JOVE video that explains it all step by step. Video link: dx.doi.org/10.3791/6940... Peroxi_SPY link: spirochrome.com/products/?_c... 🧪

🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.

Omaya Dudin 𓂆 ¦🍉¦🦠¦🔬¦@dudinlab.bsky.social · 6mo ago

At this high resolution, we also caught something new: Using the new #HAK-Actin #UExM probe, we saw that #Actin accumulates at the furrow base before compartments fully seal ➡️ they're still open at the bottom. ➡️ The #multicellular epithelium-like stage is shorter than we previously proposed.

An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™. These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green). Movie from preprint www.biorxiv.org/content/10.6... 🧪

An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™. These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green). Movie from preprint www.biorxiv.org/content/10.6... 🧪

🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁 Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT) Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology

🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁 Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT) Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology

🟢FastAct™ goes green! 🟢🔬 We launch SPY488-FastAct™ a green channel live cell actin probe SPY488-FastAct allows imaging F-actin in live and fixed cells Compatible with SPY555 and SPY650 for easy 3 color imaging. spirochrome.com/product/spy4... available at our distributors in a couple of days. 🧪

🟢FastAct™ goes green! 🟢🔬 We launch SPY488-FastAct™ a green channel live cell actin probe SPY488-FastAct allows imaging F-actin in live and fixed cells Compatible with SPY555 and SPY650 for easy 3 color imaging. spirochrome.com/product/spy4... available at our distributors in a couple of days. 🧪

1/New preprint just dropped! 🔥🔥 We investigate how the genome is destroyed in apoptotic cells in a way that prevents DNA fragments from spreading beyond the dying cell 🧬⚰ Done here at @imbavienna.bsky.social & in the super @rcollepardo.bsky.social & Rosen labs: www.biorxiv.org/content/10.6...

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday Easy to use: just dilute the probe in cell culture medium and image 30 min later! 🔗 spirochrome.com/product/spy6... 🙏 Big thanks to @lazaresaladin.bsky.social for the images. 🧪

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday Easy to use: just dilute the probe in cell culture medium and image 30 min later! 🔗 spirochrome.com/product/spy6... 🙏 Big thanks to @lazaresaladin.bsky.social for the images. 🧪

An amazing preprint by @gerlichlab.bsky.social on the fate of DNA during apoptosis. Definitely to add on your reading list! Our SPY650-DNA probe was heavily used to visualize DNA throughout the manuscript. Congratulations to the team!

Gerlich Lab@gerlichlab.bsky.social · 5mo ago

1/New preprint just dropped! 🔥🔥 We investigate how the genome is destroyed in apoptotic cells in a way that prevents DNA fragments from spreading beyond the dying cell 🧬⚰ Done here at @imbavienna.bsky.social & in the super @rcollepardo.bsky.social & Rosen labs: www.biorxiv.org/content/10.6...

SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!

Another fiery 💥 probes combination by @j-today.bsky.social, this time with SPY555-Tubulin and SPY650-FastAct_X. 🤩

Joey Tidei@j-today.bsky.social · 5mo ago

SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!

ICYMI SPY555-FastAct_X and SiR-tubulin in action here! 🤯 16h imaging of neurons 💥 Thank you @j-today.bsky.social for posting this gem!

Joey Tidei@j-today.bsky.social · 6mo ago

Cell body getting taken for a ride in this early stage neuron. Microtubules🔵 and Actin🟣 labeled and imaged for 16hr on a Zeiss LSM880. Happy #FluorescenceFriday