Zhixing Chen
@zhixingchen2.bsky.social
Assoc. Prof. at Peking University. Imaging probes, chemical biology, super-resolution imaging. https://zhixingchenlab.mysxl.cn/
The Landoni Lab opens this summer at UNIL! @fbm-unil.bsky.social 🔬 Quantitative #imaging + systems biology to decipher how #metabolic architecture & #mtDNA dynamically shape tissue development, #aging and #mitodisease. Beyond excited to fire up the lasers, open positions soon! 🧪 #mitochondria #newPI
See how PKMito dyes promote mitochondrial research😀 @spirochrome.com
Juan C. Landoni@jclandoni.bsky.social · 4mo agoOur paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]
Beware the SPY488-FastAct rollercoaster! We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct. Find out more about SPY488-FastAct: spirochrome.com/product/spy4...
Proximity labeling users: check out this study by @zhixingchen2.bsky.social and Peng Zou, which establishes how much contact-dependence vs. diffusion is responsible for biotinylation — tl;dr TurboID has a much smaller effective radius! www.nature.com/articles/s41... 1/2
Spatial barcoding reveals reaction radii and contact-dependent mechanism of proximity labeling - Nature Chemical Biology
A DNA nanoruler platform enables precise measurement of the labeling radii of TurboID and APEX2, uncovering their contact-dependent mechanisms. These findings redefine the enzymology of proximity labe...
nature.com
New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...
Congrats and thank you for using PKMito!
Itay Budin@ibudin.bsky.social · 11mo agoVery excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...
🌑Goodbye bleaching, hello brightness! ☀️ We're thrilled to introduce two new Halotag ligands featuring @zhixingchen2.bsky.social fantastic photoresistant BD-dyes! ✅ Highly photoresistant, no photoblueing ✅ Outstanding brightness ✅ Cell permeable ✅ Outperform best in class fluorophores
Check it out at PNAS today. Thank you Alexandre for the collaboration!! PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by Jing Ling, Yitong Liu, and all the collaborators! Available @spirochrome.com
Alexandre Dumoulin@axonsalex.bsky.social · last yr.Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social ! We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience tiny.cc/3qhp001
Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging: www.biorxiv.org/content/10.1... Congratulations to Julian Kompa and the entire team.
Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging
We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...
biorxiv.org
Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
nature.com
Our groundbreaking Dark Sectioning technique is now published in Nature Methods! 🎉 It supercharges existing imaging systems: 🔬 Widefield → Confocal 🔬 Single-photon → Multiphoton 🔬 2D-SIM → 3D-SIM Now integrated in Airy PolarSIM and #FlexSIM! doi.org/10.1038/s415... #Microscopy #Fluorescence
We are looking for a postdoc to study biomolecular condensates and/or mechanosensitive membrane proteins. Please help spread the news 🙏 sites.rutgers.edu/shi-lab/look...
Looking for a postdoc - Shi Lab
The Shi lab at Rutgers-New Brunswick is seeking a postdoctoral researcher to study biomolecular condensates and/or mechanosensitive membrane proteins. To see more information and apply, please go to t...
sites.rutgers.edu
Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!! www.nature.com/articles/s41... @spirochrome.com
A palette of bridged bicycle-strengthened fluorophores - Nature Methods
A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...
nature.com
Excited to share my first post on Bluesky: I’ve recently been appointed as a Professor at Goethe University Frankfurt! 🎉 The Stephan Lab will use advanced bioimaging 🔬🧫 to explore how organelles collaborate to maintain subcellular architecture and metabolism.
A comprehensive review on covalent chemical strategies for protein self-labeling from @kjohnsson.bsky.social's lab: HaloTag, SNAP-tag et al. www.annualreviews.org/content/jour...
Happy to share DeepCristae😀, a CNN for mitochondria restoration, now in @commsbio.bsky.social y.social rdcu.be/ebrAQ S.Papereux, @ludovicleconte.bsky.social, @zhixingchen2.bsky.social, J.Salamero, C.Kervrann, A.Badoual @the.3i.social @institutcurie.bsky.social #inria @france-bioimaging.bsky.social
🔔🧪 I'm deeply saddened by the passing of Michael Sheetz, a true pioneer in #mechanobiology. I'll always cherish our lovely times in NYC, Singapore, Zurich, and Sydney. My heartfelt condolences to his family and friends in this difficult time. So long, Mike 🥺😔
Mitochondria vibes only! Want to measure the inner membrane potential but unsure how or when? This review breaks it down! From protocols to pitfalls, get the tools you need to decode cellular powerhouses. ow.ly/Ejj050UsK7Y
How and when to measure mitochondrial inner membrane potentials
The scientific literature on mitochondria has increased significantly over the years due to findings that these organelles have widespread roles in the onset and progression of pathological conditions such as metabolic disorders, neurodegenerative and cardiovascular diseases, inflammation, and cancer. Researchers have extensively explored how mitochondrial properties and functions are modified in different models, often using fluorescent inner mitochondrial membrane potential (ΔΨm) probes to assess functional mitochondrial aspects such as protonmotive force and oxidative phosphorylation.
ow.ly
I always tell this "discouraging" story to the grad students on my class...
Marc Somssich@somssich.bsky.social · 2y agoDo you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇
First post on Bluesky!✨ (1/7) Far-red dopamine sensor is coming! Excited to introduce our chemigenetic DA sensor, HaloDA1.0! #Dopamine #FarRedSensors, #GRAB www.biorxiv.org/content/10.1...
biorxiv.org
How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling. www.biorxiv.org/content/10.1... Special thanks to @aliceyting.bsky.social for discussions
Starting the week strong! Cheering myself up for the next challenges with 15 min of axonal growth cone dynamic madness. F-actin (cyan) and membrane (magenta) show their dance. One image taken every 10 s.🧠🔬 #MicroscopyMonday #LiveImaging #Neuroscience #Microscopy
I feel the science-to-nonsense ratio is much better on this site: Enjoy these jittery mitochondria sifting through axons of patient-derived motor neurons thanks to the pkMito probe from @spirochrome.com #neuroscience #science #microscopy #bioimaging #mitochondria
