New #cryo-EM #preprint- 'Structural mechanisms of drebrin-mediated F-actin network modulation'. Weilong impressively classified out two small #actin binding domains competing for the same surface on #F-actin, one of which also exists in two conformations! #drebrin www.biorxiv.org/content/10.6...

SPIN90 and #YanCao strike again! First NPF to only activate a subset of Arp2/3 iso-complexes to regulate lamellipodial actin & protrusion efficiency. Great collaboration with @romet-jegou-lab.bsky.social #ManosMavrakis polarized fluorescence microscopy rocks 🤘💥🎉 www.biorxiv.org/content/10.6...

SPIN90 modulates the architecture of lamellipodial actin in an ARPC5L dependent fashion.

When stimulated by nucleation-promoting factors such as WAVE, the Arp2/3 complex generates branched actin networks. In contrast, when activated by SPIN90, the Arp2/3 complex generates linear actin fil...

biorxiv.org

Now for something completely different - Way lab does immunology - check out our @science.org paper - Branched actin networks mediate macrophage-dependent host-microbiota homeostasis. The work was driven by the 2 first authors @luiz90vasc.bsky.social @shainahuang.bsky.social

Our paper on Arp2/3-mediated bidirectional actin assembly is out @natsmb.nature.com 🚀 After great reviews & community feedback, this version includes new insights beyond the preprint:) Huge thanks to all co-authors!!! @drmichaelway.bsky.social @romet-jegou-lab.bsky.social @carolynmoores1.bsky.social

Michael Way@drmichaelway.bsky.social · 11mo ago

And now for something completely different @tianyang22.bsky.social & #YanCao uncover of how SPIN90 dimers activate Arp2/3 to nucleate bidirectional linear actin filaments. Great collaboration with @romet-jegou-lab.bsky.social & @carolynmoores1.bsky.social #Cryo-EM #Arp2/3 rdcu.be/eGoH4

Another wonderful collaboration with @alanbrownhms.bsky.social lab 👇 Zhang lab (co-first author @qingweiniu.bsky.social) contribute the doublet structure from Crithidia fasciculata, a model organism that infects mosquitoes.

Matt Doran@matthdoran.bsky.social · last yr.

I am super excited to share our latest work on the structures of doublet microtubules from trypanosomatid parasites, the causative agents of leishmaniasis, Chagas disease, and African sleeping sickness. tinyurl.com/48sh3xn5

Excited to announce the 10th "Choanoflagellates & Friends Workshop" over May 20-22 in our own Institut Pasteur in Paris: 2025choanocon.wordpress.com Registrations open til March 15, abstract til March 5. Pre-conference event for students/postdocs on 19/05. Plenty of slots for selected talks/posters!

Working on actin cytoskeleton and Arp2/3 complex? Check out our latest work～https://www.biorxiv.org/content/10.1101/2025.01.31.635869v1 In this work, we found SPIN90 forms a dimer, facilitating bidirectional actin filament nucleation by activating two Arp2/3 complex #Cryo-EM #Actin #Arp2/3

Michael Way@drmichaelway.bsky.social · 2y ago

Arp2/3 nucleates bidirectional linear actin filaments when activated by SPIN90 !!! www.biorxiv.org/content/10.1...