Will Scott
@wsctt.bsky.social
Actin cytoskeleton, protein engineering, synthetic chemistry. Interested in vascular healing. Postdoc 🔬🏳️🌈 He/Him. Where there's a Will there's a way
3-iodotyrosine fluorescent proteins for improved expansion microscopy #micropublication #biology #data #caltechlibrary #Zebrafish #Escherichiacoli #Biochemistry #Methods #NewFinding #Methodology https://micropublication.org/journals/biology/micropub-biology-002234
Recently we were trying to change the colour of superfolder GFP and accidentally engineered a great probe for expansion microscopy! Read about it in our first micropublication - lnkd.in/eCxYcCJZ
@wsctt.bsky.social and Nimo Abdullah from @sporemohan.bsky.social lab demonstrate the potential of 3-iodotyrosine containing FPs for expansion microscopy 🔬🐟🌈 Happy to have played a small role in this exciting project Findings available @micropub7n.bsky.social micropublication.org/journals/bio...
New from Mishima & Balasubramanian (@sporemohan.bsky.social) labs in @warwickmed.bsky.social: “Actin arginylation alters myosin engagement & F-actin patterning despite structural conservation.” Explore the physiology of #arginylated #actin 👉 rupress.org/jcb/article/...
Actin arginylation alters myosin engagement and F-actin patterning despite structural conservation | Journal of Cell Biology | Rockefeller University Press
Actin is vital for cell functions and is regulated by posttranslational modifications like arginylation. The structure of arginylated β-actin (R-β-actin) i
rupress.org
Our new paper is published in the Journal of Chemical Biology! 🏮 We share StayRose, a red version of StayGold 🏮 StayRose has an unnatural amino acid-based chromophore, and has the same immense photobleach-resistance as StayGold 💡
StayRose: A photostable StayGold derivative redshifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality images in microscopy. StayGold, a novel dimeric GFP recently monomerized through sequence engineering, addresses thi...
jbc.org
mStayRose is published in JBC 🥳🎉 Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable: www.jbc.org/article/S002...
StayRose: a photostable StayGold derivative red-shifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality images in microscopy. StayGold, a novel dimeric green fluorescent protein recently monomerised through sequence engi...
jbc.org
⚗️ Take a read of our new preprint presenting a one-step method for conjugating strained alkyne groups onto fluorescent groups 🧪 Drop me a message if you're interested in trying it out! doi.org/10.26434/che...
Simple conjugation of strained alkynes and fluorescent dyes
Click reactions between strained alkyne and azide groups allow bioconjugation of fluorescent dyes onto proteins bearing unnatural amino acids. Strained alkyne dyes for protein imaging are available bu...
doi.org
I was privileged to be interviewed by Biology Open last week about our new paper, what inspired me to become a scientist, and how research is as creative as any artform 🔬 Take a read here: doi.org/10.1242/bio....
🔬 Take a read of our paper out today, presenting improved tools for comparing human tropomyosin isoforms in live cells 🧬 My first publication as lead author 🥳 Please reach out if you'd like to give any of these tools a try 🔬 doi.org/10.1242/bio....
Fluorescent protein tags for human tropomyosin isoform comparison
Summary: Green and red fluorescent fusion proteins with lengthy and flexible polypeptide linkers readily allow improved visualisation and comparison of tropomyosin isoform distribution in live human c...
doi.org
Fluorescent protein tags for human tropomyosin isoform comparison https://www.biorxiv.org/content/10.1101/2025.02.25.640032v1
Fluorescent protein tags for human tropomyosin isoform comparison https://www.biorxiv.org/content/10.1101/2025.02.25.640032v1
Take a read of our brand new preprint 🥼 We built a toolbox of new live cell imaging probes for comparing human isoforms of the surprisingly mysterious cytoskeletal protein tropomyosin 🔬 This started as a side project during my PhD and grew into something quite huge! 🧫
Fluorescent protein tags for human tropomyosin isoform comparison
Tropomyosin is an important actin cytoskeletal protein underpinning processes such as muscle contraction, cell shape and cell division. Defects in tropomyosin function can lead to diseases, including ...
biorxiv.org
StayRose: a photostable StayGold derivative red-shifted by genetic code expansion https://www.biorxiv.org/content/10.1101/2024.12.13.628370v1
Our new preprint on StayRose is out now - we used an unnatural amino acid to produce the first red version of the super photostable fluorescent protein StayGold 🥰 My first as lead author! 🥳
StayRose: a photostable StayGold derivative red-shifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality images in microscopy. StayGold, a novel dimeric green fluorescent protein recently monomerised through sequence engi...
biorxiv.org
StayGold red-shifted by non-natural aminoacid mutagenesis. see our reprint, another fruitful collaboration with @midbody @Allister_Crow. Well done Esther & Will Scott first authors. This tool is being developed further for imaging. Watch this space. www.biorxiv.org/content/10.1...
biorxiv.org
StayRose: a photostable StayGold derivative red-shifted by genetic code expansion https://www.biorxiv.org/content/10.1101/2024.12.13.628370v1
Glad to connect with everyone! I'm a synthetic biologist and chemist imaging the actin cytoskeleton in Mohan Balasubramanian's group (University of Warwick) on an end-of-PhD transition fellowship. Here are my yeast from throughout my PhD (can you see which weeks I was particularly stressed 😂)
