We present: Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution www.biorxiv.org/content/10.6...
Akaash Kumar
@akaashkumar.bsky.social
Interdisciplinary scientist interested in technology development, microscopy and biological sciences. Multispectral live cell imaging of membrane trafficking. MRC LMB, Cambridge, UK.
We've updated our cleared-tissue direct-view oblique plane microscopy preprint to include our latest resolution-enhancing results. Now the Nyquist sampling of our camera is no longer a limitation and so we obtain a diffraction-limited 2 μm lateral resolution! www.biorxiv.org/content/10.6...
James Manton@jamesdmanton.bsky.social · 3mo agoWe present: Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution www.biorxiv.org/content/10.6...
Great work from Lucas, the @simonbullock11.bsky.social lab and collaborators uncovering the relocalisation of organelles by dynein when cells experience stress! Check out the pre-print 👇🏽
Simon Bullock@simonbullock11.bsky.social · 6mo agoDelighted to announce the posting of our latest preprint, which links stress-induced relocalisation of multiple organelles by dynein to adaptive changes in gene expression in the nucleus (1/n) 🧵 www.biorxiv.org/content/10.6...
Myself and @immunoah.bsky.social are looking to hire a postdoctoral scientist to build a next-generation high-throughput high-resolution light sheet microscope for imaging entire cleared organs/tissues and to further develop live & fixed tissue clearing methods. www.nature.com/naturecareer...
MRC Laboratory of Molecular Biology@mrclmb.ac.uk · 6mo agoInterested in instrument development & optical microscopy? Join @immunoah.bsky.social & @jamesdmanton.bsky.social developing high-throughput light sheet microscopy to image entire tissues & progressing tissue clearing methods www.nature.com/naturecareer... Closes 07 APR #PostdocJobs #neuroimmunology
🚨Publication alert🚨 My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
Multispectral live-cell imaging with uncompromised spatiotemporal resolution - Nature Photonics
A tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with...
nature.com
We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:
bioRxiv Biophysics@biorxiv-biophys.bsky.social · 12mo agoMaximising imaging volumes of expanded tissues for inverted fluorescence microscopy https://www.biorxiv.org/content/10.1101/2025.09.12.675899v1
Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!
Thank you very much for the feature and the article!! A nice surprise before the weekend...
Biomedical Picture of the Day BPoD@bpodaily.bsky.social · 2y agoNew approach with an unmixing algorithm & 8-channel image acquisition enables multispectral live-cell imaging 📷: @akaashkumar.bsky.social et al @jamesdmanton.bsky.social @cellbiol-mrclmb.bsky.social @mrclmb.bsky.social in @biorxivpreprint.bsky.social ➡️: bpod.org.uk/archive/2025... with Ant Lewis
🚨 Paper Alert 🚨 Our preprint describing Elongator as a selective microtubule polymerase that rewrites PTMs on microtubules is now published! Check it out: www.embopress.org/doi/full/10....
Elongator is a microtubule polymerase selective for polyglutamylated tubulin | The EMBO Journal
imageimageElongator, an established tRNA-modifying complex, can also stabilize microtubules during asymmetric cell division. This study shows that Elongator subcomplexes achieve this by binding to mic...
embopress.org
Congrats to @juliemiesch.bsky.social who was awarded a Postdoc Mobility grant by @snf-ch.bsky.social to support her research for two years. Her postdoc research will focus on the biophysical regulation of water buffering in the cell.
Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology www.biorxiv.org/content/10.1...
Congrats to @akaashkumar.bsky.social who has won the PhD Award from the Cambridge Centre for Physical Biology for his work developing a novel microscopy system with @deriverylab.bsky.social & @jamesdmanton.bsky.social in @cellbiol-mrclmb.bsky.social. www.physbiol.cam.ac.uk/akaash-kumar #LMBintheNews
Simultaneous multispectral imaging of seven organelles in live cells using our multispectral spinning disk confocal microscope! #FluorescenceFriday You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
Very happy that my work on developing multispectral live cell imaging technology has been recognised by the Cambridge Centre for Physical Biology! Thanks for the award!
Derivery Lab - MRC LMB@deriverylab.bsky.social · 2y agoCongrats to @akaashkumar.bsky.social , a postdoc in the lab, for winning the PhD Award from the Cambridge Centre for Physical Biology. His PhD research worked on the development of a multispectral imaging system to image up to 8 fluorophores similtaneously for tackling complex biological questions!
In June, @akaashkumar.bsky.social and @jamesdmanton.bsky.social had their Multispectral live-cell imaging paper released on to the BioRxiv www.biorxiv.org/content/10.1...
Multispectral live-cell imaging with uncompromised spatiotemporal resolution
Multispectral imaging is an established method to extend the number of colours usable in fluorescence imaging beyond the typical limit of three or four, but standard approaches are poorly suited to li...
biorxiv.org
Then, in September @akaashkumar.bsky.social passed his PhD viva, becoming the latest doctor to emerge from the lab! He then won the RMS Early Career Award 2024 in October.
Later in October, the LMB had their yearly annual symposium with Post-doc Lara Kruger winning the Steitz Prize for her talk and @akaashkumar.bsky.social winning the Perutz Prize for his PhD work!
Some personal news: my wife and I are finally homeowners! We got the keys to our home in Cambridge today, very exciting times!
