Thrilled to see this work published! Dr. Pierre and @angelopilotti.bsky.social developed an accessible flow-cytometry approach that simultaneously profiles all three branches of the Unfolded Protein Response (UPR) in nuclear suspensions at single-cell resolution. Congrats to all authors!

Angelo Pilotti@angelopilotti.bsky.social · 2mo ago

🎉 New paper out! We developed SNUPR, a flow-cytometry approach that profiles all three branches of the Unfolded Protein Response (UPR) at single-cell resolution in nuclear suspensions. 📄 doi.org/10.1038/s443... @embomolmed.org @ciml.bsky.social @cnrs.fr @univ-amu.fr

🎉 New paper out! We developed SNUPR, a flow-cytometry approach that profiles all three branches of the Unfolded Protein Response (UPR) at single-cell resolution in nuclear suspensions. 📄 doi.org/10.1038/s443... @embomolmed.org @ciml.bsky.social @cnrs.fr @univ-amu.fr

Single-nuclei UPR profiling by flow cytometry reveals bortezomib resistance mechanisms in multiple myeloma - EMBO Molecular Medicine

The unfolded protein response (UPR) is a stress-adaptation pathway and therapeutic target in cancer, yet its pro-survival versus pro-death outcome is difficult to predict because the three ER sensors, PERK, IRE1α, and ATF6, are highly interconnected. Transcriptomic analyses identified sensor-specific gene signatures associated with patient survival across malignancies, and indicated that low IRE1α activity (low XBP1 signature or higher expression of RIDD targets) correlates with improved outcome. We developed SNUPR (single nuclei analysis of the unfolded protein response), an accessible flow cytometry approach that profiles all three branches in nuclear suspensions. SNUPR reveals marked heterogeneity of UPR activation across cancer cell lines that cannot be inferred from sensor expression. This heterogeneity is derived from differences in the strength and duration of PERK-mediated translational inhibition, which gates downstream translation-dependent IRE1α and ATF6 transcriptional programs. Finally, in multiple myeloma, we show that bortezomib-tolerant cells depend on IRE1α activity for survival, linking UPR state to proteasome-inhibitor resistance and positioning SNUPR to guide branch-selective targeting.

doi.org

🧪Come with me for a very normal day of work and get to know about my research project! @immerge.bsky.social @ciml.bsky.social @cnrs.fr

🌍 IMMERGE beyond the lab! On Sept 20, @angelopilotti.bsky.social (Pierre's lab) spoke at a conference in Fondi, Italy—organized by local associations to bring science closer to the public. 👏 He talked about autoimmune diseases & shared PhD insights on stress responses and immunity #IMMERGE #MSCA

L'amour c'est beau Et d'ailleurs, nous aussi qu'est-ce qu'on est beaux Amants, amis, garçons ou filles, tout ce qui s'ensuit 🏳️‍🌈 #marseillepride2025

Delighted to visit CIML in Marseille Hosted by Philippe Pierre for the talk and examine @angelopilotti.bsky.social for his PhD first year

🔵New paper alert🔵 ❗️Barros and Ferreira show that induction of the ISR by SubAB drives type-I IFN expression in pDCs via STING 🎉Glad to have contributed to this work. Thanks to Philippe Pierre and @immerge.bsky.social 👉🏻Full article on @pnas.org at www.pnas.org/doi/10.1073/pnas.2421258122

🔬Exciting IMMERGE research in action! 🔬 IMMERGE PhD fellow @angelopilotti.bsky.social presented his work at the PhD workshop at @ciml.bsky.social! 📊✨ Great to see our researchers sharing knowledge & advancing the field! 👏 #IMMERGE #MSCA #IEI #Immunology #ISR #DendriticCells #SLE #SAVI

Angelo Pilotti@angelopilotti.bsky.social · last yr.

🔬 Exploring the role of the Integrated Stress Response in dendritic cells and interferonopathies 🔬 📊Last week, I had the opportunity of presenting my research project during the PhD workshop at @ciml.bsky.social. (1/7)

🔬 Exploring the role of the Integrated Stress Response in dendritic cells and interferonopathies 🔬 📊Last week, I had the opportunity of presenting my research project during the PhD workshop at @ciml.bsky.social. (1/7)

just this morning @angelopilotti.bsky.social presented his poster at the @ciml.bsky.social #PhD workshop! Super interesting project linking stress responses with interferonopathies! very curious to follow up this project!

IMMERGE MSCA DN@immerge.bsky.social · last yr.

🔬 Meet the Researchers Behind IMMERGE! 🔬 This week: @angelopilotti.bsky.social & Dr. Pierre from @cnrs.fr! Angelo will study the functional impact of EIF2AK mutants in dendritic cells & interferonopathies🧬👨‍🔬 Stay tuned for more IMMERGE scientists! 🚀 #IMMERGE #MSCA #IEI #DCs #Interferonopathies

Honored to be working on the Characterization of rare gene variants affecting the Integrated Stress Response in dendritic cells and interferonopathies at @ciml.bsky.social in P. Pierre’s lab. And grateful to be part of the @immerge.bsky.social program! #ISR #IntegratedStressResponse #SAVI #SLE

IMMERGE MSCA DN@immerge.bsky.social · last yr.

🔬 Meet the Researchers Behind IMMERGE! 🔬 This week: @angelopilotti.bsky.social & Dr. Pierre from @cnrs.fr! Angelo will study the functional impact of EIF2AK mutants in dendritic cells & interferonopathies🧬👨‍🔬 Stay tuned for more IMMERGE scientists! 🚀 #IMMERGE #MSCA #IEI #DCs #Interferonopathies

🔬 Meet the Researchers Behind IMMERGE! 🔬 This week: @angelopilotti.bsky.social & Dr. Pierre from @cnrs.fr! Angelo will study the functional impact of EIF2AK mutants in dendritic cells & interferonopathies🧬👨‍🔬 Stay tuned for more IMMERGE scientists! 🚀 #IMMERGE #MSCA #IEI #DCs #Interferonopathies

💼 I participated in the inaugural @immerge.bsky.social Symposium. 🙏 I am especially grateful to Philippe Pierre, Vittoria Spinosa, @eballestar.bsky.social, and José Luis Sardina. #MSCA #DoctoralNetworks #HorizonEurope #MSCActions #EU_Commission www.linkedin.com/posts/angelo...

Angelo Pilotti on LinkedIn: #msca #doctoralnetworks #horizoneurope #integratedstressresponse…

💼 I recently had the privilege of participating in the inaugural IMMERGE Symposium as a doctoral candidate within this network. 🤝 The three-day event was an…

linkedin.com

🙋 Are you studying the Unfolded Protein Response (UPR) and/or the Integrated Stress Response (ISR)? 👇🏻 Let’s all gather here! Thank you @profmarciniak.bsky.social! #UPR #ISR #UnfoldedProteinResponse #IntegratedStressResponse

Stefan Marciniak@profmarciniak.bsky.social · 2y ago

Arise, UPR & ISR scientists ! I will have missed many, so please tag below go.bsky.app/8VzRKLW

Thinking of making an Integrated Stress Response starter pack. Suggested names? @wisemanlab.bsky.social @ericchevet.bsky.social @angelopilotti.bsky.social @joechambers.bsky.social @glennmasson.bsky.social There must be more here